NMU_RAT
ID NMU_RAT Reviewed; 174 AA.
AC P12760;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Neuromedin-U;
DE Contains:
DE RecName: Full=Neuromedin precursor-related peptide 36 {ECO:0000303|PubMed:28874765};
DE Short=NURP36 {ECO:0000303|PubMed:28874765};
DE Contains:
DE RecName: Full=Neuromedin precursor-related peptide 33 {ECO:0000303|PubMed:28874765};
DE Short=NURP33 {ECO:0000303|PubMed:28874765};
DE Contains:
DE RecName: Full=Neuromedin-U-23;
DE Short=NmU-23;
DE Flags: Precursor;
GN Name=Nmu;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar;
RX PubMed=1448109; DOI=10.1210/mend.6.10.1448109;
RA Lo G., Legon S., Austin C., Wallis S., Wang Z., Bloom S.R.;
RT "Characterization of complementary DNA encoding the rat neuromedin U
RT precursor.";
RL Mol. Endocrinol. 6:1538-1544(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Sprague-Dawley;
RA Sharma S.K., Lo G., Szpirer C., Bloom S.R.;
RT "Molecular cloning, characterization and chromosomal localization of the
RT rat neuromedin U (NmU) gene.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 106-141, FUNCTION (NEUROMEDIN-U-23; NEUROMEDIN
RP PRECURSOR-RELATED PEPTIDE 33 AND NEUROMEDIN PRECURSOR-RELATED PEPTIDE 36),
RP TISSUE SPECIFICITY, PROTEOLYTIC PROCESSING, MASS SPECTROMETRY, AND
RP OXIDATION AT MET-141.
RC TISSUE=Brain {ECO:0000303|PubMed:28874765}, and
RC Small intestine {ECO:0000303|PubMed:28874765};
RX PubMed=28874765; DOI=10.1038/s41598-017-10319-9;
RA Mori K., Ida T., Fudetani M., Mori M., Kaiya H., Hino J., Nakahara K.,
RA Murakami N., Miyazato M., Kangawa K.;
RT "Identification of neuromedin U precursor-related peptide and its possible
RT role in the regulation of prolactin release.";
RL Sci. Rep. 7:10468-10468(2017).
RN [4]
RP PROTEIN SEQUENCE OF 144-166, AND AMIDATION AT ASN-166.
RC TISSUE=Small intestine;
RX PubMed=3178840; DOI=10.1016/s0006-291x(88)80848-9;
RA Minamino N., Kangawa K., Honzawa M., Matsuo H.;
RT "Isolation and structural determination of rat neuromedin U.";
RL Biochem. Biophys. Res. Commun. 156:355-360(1988).
RN [5]
RP PROTEIN SEQUENCE OF 144-166.
RX PubMed=3411332; DOI=10.1111/j.1471-4159.1988.tb01837.x;
RA Conlon J.M., Domin J., Thim L., Dimarzo V., Morris H.R., Bloom S.R.;
RT "Primary structure of neuromedin U from the rat.";
RL J. Neurochem. 51:988-991(1988).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=7916966; DOI=10.1677/jme.0.0120257;
RA Austin C., Oka M., Nandha K.A., Legon S., Khandan-Nia N., Lo G.,
RA Bloom S.R.;
RT "Distribution and developmental pattern of neuromedin U expression in the
RT rat gastrointestinal tract.";
RL J. Mol. Endocrinol. 12:257-263(1994).
RN [7]
RP PROTEOLYTIC PROCESSING.
RX PubMed=15635449; DOI=10.1038/sj.emboj.7600526;
RA Mori K., Miyazato M., Ida T., Murakami N., Serino R., Ueta Y., Kojima M.,
RA Kangawa K.;
RT "Identification of neuromedin S and its possible role in the mammalian
RT circadian oscillator system.";
RL EMBO J. 24:325-335(2005).
CC -!- FUNCTION: [Neuromedin-U-23]: Ligand for receptors NMUR1 and NMUR2
CC (PubMed:28874765). Receptor-binding is very tight if not irreversible
CC and triggers an increase in the cytosolic Ca(2+) concentration
CC (PubMed:28874765). Stimulates muscle contractions of specific regions
CC of the gastrointestinal tract. In rat, NMU stimulates contractions of
CC stomach circular muscle. {ECO:0000269|PubMed:28874765}.
CC -!- FUNCTION: [Neuromedin precursor-related peptide 33]: Does not function
CC as a ligand for either NMUR1 or NMUR2 (PubMed:28874765). Indirectly
CC induces prolactin release although its potency is much lower than that
CC of neuromedin precursor-related peptide 36 (PubMed:28874765).
CC {ECO:0000269|PubMed:28874765}.
CC -!- FUNCTION: [Neuromedin precursor-related peptide 36]: Does not function
CC as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin
CC release from lactotroph cells in the pituitary gland, probably via the
CC hypothalamic dopaminergic system. {ECO:0000269|PubMed:28874765}.
CC -!- SUBCELLULAR LOCATION: [Neuromedin-U-23]: Secreted
CC {ECO:0000305|PubMed:28874765}.
CC -!- TISSUE SPECIFICITY: Expressed throughout the gastrointestinal tract
CC with highest levels in the duodenum and jejunum (PubMed:7916966). Low
CC levels in spinal cord, hypothalamus, and stomach (PubMed:7916966).
CC Neuromedin-U-23: Expressed in the small intestine and the pituitary
CC gland (at protein level) (PubMed:28874765). Neuromedin precursor-
CC related peptides: Expressed in pituitary gland and small intestine (at
CC protein level) (PubMed:28874765). {ECO:0000269|PubMed:28874765,
CC ECO:0000269|PubMed:7916966}.
CC -!- MASS SPECTROMETRY: [Neuromedin precursor-related peptide 33]:
CC Mass=3759.8; Method=MALDI; Note=Neuromedin U precursor-related peptide
CC 33.; Evidence={ECO:0000269|PubMed:28874765};
CC -!- MASS SPECTROMETRY: [Neuromedin precursor-related peptide 36]:
CC Mass=4202.2; Method=MALDI; Note=Neuromedin U precursor-related peptide
CC 36.; Evidence={ECO:0000269|PubMed:28874765};
CC -!- MASS SPECTROMETRY: [Neuromedin precursor-related peptide 36]:
CC Mass=4218.8; Method=MALDI; Note=Neuromedin U precursor-related peptide
CC 36, oxidized.; Evidence={ECO:0000269|PubMed:28874765};
CC -!- SIMILARITY: Belongs to the NmU family. {ECO:0000305}.
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DR EMBL; M94555; AAA41717.1; -; mRNA.
DR EMBL; AF222764; AAF64427.1; -; Genomic_DNA.
DR EMBL; AF222755; AAF64427.1; JOINED; Genomic_DNA.
DR EMBL; AF222756; AAF64427.1; JOINED; Genomic_DNA.
DR EMBL; AF222759; AAF64427.1; JOINED; Genomic_DNA.
DR EMBL; AF222760; AAF64427.1; JOINED; Genomic_DNA.
DR EMBL; AF222761; AAF64427.1; JOINED; Genomic_DNA.
DR EMBL; AF222762; AAF64427.1; JOINED; Genomic_DNA.
DR EMBL; AF222763; AAF64427.1; JOINED; Genomic_DNA.
DR PIR; A45356; A45356.
DR RefSeq; NP_071575.1; NM_022239.2.
DR AlphaFoldDB; P12760; -.
DR BioGRID; 248921; 2.
DR STRING; 10116.ENSRNOP00000002941; -.
DR PaxDb; P12760; -.
DR GeneID; 63887; -.
DR KEGG; rno:63887; -.
DR UCSC; RGD:68388; rat.
DR CTD; 10874; -.
DR RGD; 68388; Nmu.
DR VEuPathDB; HostDB:ENSRNOG00000002164; -.
DR eggNOG; ENOG502S1QB; Eukaryota.
DR HOGENOM; CLU_090356_0_0_1; -.
DR InParanoid; P12760; -.
DR OMA; CFMVMGI; -.
DR OrthoDB; 1515830at2759; -.
DR PhylomeDB; P12760; -.
DR TreeFam; TF338319; -.
DR Reactome; R-RNO-375276; Peptide ligand-binding receptors.
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR PRO; PR:P12760; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000002164; Expressed in duodenum and 10 other tissues.
DR ExpressionAtlas; P12760; baseline and differential.
DR Genevisible; P12760; RN.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0043195; C:terminal bouton; IDA:RGD.
DR GO; GO:0042922; F:neuromedin U receptor binding; IDA:MGI.
DR GO; GO:0031839; F:type 1 neuromedin U receptor binding; IDA:UniProtKB.
DR GO; GO:0031840; F:type 2 neuromedin U receptor binding; IDA:UniProtKB.
DR GO; GO:0042755; P:eating behavior; IDA:RGD.
DR GO; GO:0097009; P:energy homeostasis; IDA:UniProtKB.
DR GO; GO:0001696; P:gastric acid secretion; IMP:RGD.
DR GO; GO:1903999; P:negative regulation of eating behavior; IDA:UniProtKB.
DR GO; GO:2000252; P:negative regulation of feeding behavior; IDA:UniProtKB.
DR GO; GO:0060455; P:negative regulation of gastric acid secretion; IDA:UniProtKB.
DR GO; GO:0120061; P:negative regulation of gastric emptying; IDA:UniProtKB.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IDA:MGI.
DR GO; GO:0009648; P:photoperiodism; IEP:RGD.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:UniProtKB.
DR GO; GO:0010460; P:positive regulation of heart rate; IDA:UniProtKB.
DR GO; GO:0031652; P:positive regulation of heat generation; IDA:UniProtKB.
DR GO; GO:0046887; P:positive regulation of hormone secretion; IMP:RGD.
DR GO; GO:1902722; P:positive regulation of prolactin secretion; IDA:UniProtKB.
DR GO; GO:1904058; P:positive regulation of sensory perception of pain; IDA:UniProtKB.
DR GO; GO:0045987; P:positive regulation of smooth muscle contraction; IDA:UniProtKB.
DR GO; GO:0120069; P:positive regulation of stomach fundus smooth muscle contraction; IDA:UniProtKB.
DR GO; GO:0050806; P:positive regulation of synaptic transmission; IMP:RGD.
DR GO; GO:0003084; P:positive regulation of systemic arterial blood pressure; IDA:UniProtKB.
DR GO; GO:0045187; P:regulation of circadian sleep/wake cycle, sleep; IDA:UniProtKB.
DR GO; GO:0060259; P:regulation of feeding behavior; ISO:RGD.
DR GO; GO:2000821; P:regulation of grooming behavior; IDA:UniProtKB.
DR GO; GO:0019233; P:sensory perception of pain; IMP:RGD.
DR GO; GO:0001659; P:temperature homeostasis; ISO:RGD.
DR InterPro; IPR018070; Neuromedin-U_amidation-site.
DR InterPro; IPR042384; NMU.
DR InterPro; IPR008200; NMU_C.
DR PANTHER; PTHR15390; PTHR15390; 1.
DR Pfam; PF02070; NMU; 1.
DR SMART; SM00084; NMU; 1.
DR PROSITE; PS00967; NMU; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Oxidation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..37
FT /evidence="ECO:0000255"
FT PROPEP 38..105
FT /evidence="ECO:0000305|PubMed:28874765"
FT /id="PRO_0000019780"
FT PEPTIDE 106..141
FT /note="Neuromedin precursor-related peptide 36"
FT /evidence="ECO:0000269|PubMed:28874765"
FT /id="PRO_0000445769"
FT PEPTIDE 106..138
FT /note="Neuromedin precursor-related peptide 33"
FT /evidence="ECO:0000269|PubMed:28874765"
FT /id="PRO_0000445770"
FT PEPTIDE 144..166
FT /note="Neuromedin-U-23"
FT /id="PRO_0000019781"
FT PROPEP 170..174
FT /id="PRO_0000019782"
FT MOD_RES 141
FT /note="Methionine sulfoxide; partial"
FT /evidence="ECO:0000269|PubMed:28874765"
FT MOD_RES 166
FT /note="Asparagine amide"
FT /evidence="ECO:0000269|PubMed:3178840"
SQ SEQUENCE 174 AA; 19403 MW; 674CF5A2846FF3FF CRC64;
MSRAANRRPG LSAGQLAAAT ASPLLSLLLL LACCADACRG TPISPQRLPP EQELQLWNEI
PEACASFLSV DSQPQASVAL RKLCRVLMEI FQKPQEQTEK DNAKRFLFHY SKTQKLGNSN
VVSSVVHPLL QLVPQLHERR MKRYKVNEYQ GPVAPSGGFF LFRPRNGKRS TSFI
