NNF1_YEAST
ID NNF1_YEAST Reviewed; 201 AA.
AC P47149; D6VWT1; Q06779;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 3.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Kinetochore-associated protein NNF1;
GN Name=NNF1; OrderedLocusNames=YJR112W; ORFNames=J2011;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9247195; DOI=10.1242/jcs.110.14.1615;
RA Shan X., Xue Z., Euskirchen G., Melese T.;
RT "NNF1 is an essential yeast gene required for proper spindle orientation,
RT nucleolar and nuclear envelope structure and mRNA export.";
RL J. Cell Sci. 110:1615-1624(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=12455957; DOI=10.1128/ec.1.2.229-240.2002;
RA Euskirchen G.M.;
RT "Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a new group of proteins important for
RT chromosome segregation in Saccharomyces cerevisiae.";
RL Eukaryot. Cell 1:229-240(2002).
RN [6]
RP IDENTIFICATION IN THE MIND COMPLEX.
RX PubMed=14602074; DOI=10.1016/s1534-5807(03)00322-8;
RA Pinsky B.A., Tatsutani S.Y., Collins K.A., Biggins S.;
RT "An Mtw1 complex promotes kinetochore biorientation that is monitored by
RT the Ipl1/Aurora protein kinase.";
RL Dev. Cell 5:735-745(2003).
RN [7]
RP IDENTIFICATION IN THE MIND COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=14657030; DOI=10.1093/emboj/cdg636;
RA Scharfenberger M., Ortiz J., Grau N., Janke C., Schiebel E., Lechner J.;
RT "Nsl1p is essential for the establishment of bipolarity and the
RT localization of the Dam-Duo complex.";
RL EMBO J. 22:6584-6597(2003).
RN [8]
RP IDENTIFICATION IN THE MIND COMPLEX, AND FUNCTION OF THE MIND COMPLEX.
RX PubMed=14633972; DOI=10.1101/gad.1144403;
RA De Wulf P., McAinsh A.D., Sorger P.K.;
RT "Hierarchical assembly of the budding yeast kinetochore from multiple
RT subcomplexes.";
RL Genes Dev. 17:2902-2921(2003).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [10]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Acts as essential component of the kinetochore MIND complex,
CC which is required for the spindle checkpoint and kinetochore integrity.
CC MIND plays a role in establishing a bipolar spindle-kinetochore
CC interaction by joining kinetochore subunits contacting DNA to those
CC contacting microtubules. NNF1 is required for a number of nuclear
CC functions. Cells depleted of NNF11 or containing a temperature-
CC sensitive NNF1 mutation have elongated microtubules and become bi- and
CC multinucleate. They also have a fragmented nucleolus and accumulate
CC poly(A)+ RNA inside the nucleus. {ECO:0000269|PubMed:12455957,
CC ECO:0000269|PubMed:14633972}.
CC -!- SUBUNIT: Component of the MIND kinetochore complex, which is composed
CC of at least MTW1, NNF1, NSL1 and DSN1. {ECO:0000269|PubMed:12455957,
CC ECO:0000269|PubMed:14602074, ECO:0000269|PubMed:14633972,
CC ECO:0000269|PubMed:14657030}.
CC -!- INTERACTION:
CC P47149; P39731: MTW1; NbExp=6; IntAct=EBI-12098, EBI-11606;
CC P47149; P39946: PAC1; NbExp=3; IntAct=EBI-12098, EBI-12838;
CC P47149; P32908: SMC1; NbExp=6; IntAct=EBI-12098, EBI-17402;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12455957}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:12455957,
CC ECO:0000269|PubMed:14657030}. Note=Associated with the kinetochore
CC (PubMed:12455957, PubMed:14657030).
CC -!- MISCELLANEOUS: Present with 2070 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X93350; CAA63725.1; -; Genomic_DNA.
DR EMBL; Z49612; CAA89642.1; -; Genomic_DNA.
DR EMBL; AY558256; AAS56582.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08897.1; -; Genomic_DNA.
DR PIR; S57135; S57135.
DR RefSeq; NP_012646.3; NM_001181770.3.
DR AlphaFoldDB; P47149; -.
DR SMR; P47149; -.
DR BioGRID; 33868; 255.
DR ComplexPortal; CPX-1186; Nuclear MIS12/MIND complex.
DR DIP; DIP-3017N; -.
DR IntAct; P47149; 23.
DR MINT; P47149; -.
DR STRING; 4932.YJR112W; -.
DR iPTMnet; P47149; -.
DR MaxQB; P47149; -.
DR PaxDb; P47149; -.
DR PRIDE; P47149; -.
DR EnsemblFungi; YJR112W_mRNA; YJR112W; YJR112W.
DR GeneID; 853576; -.
DR KEGG; sce:YJR112W; -.
DR SGD; S000003873; NNF1.
DR VEuPathDB; FungiDB:YJR112W; -.
DR eggNOG; ENOG502RZTQ; Eukaryota.
DR HOGENOM; CLU_117280_0_0_1; -.
DR InParanoid; P47149; -.
DR OMA; SSCFPEY; -.
DR BioCyc; YEAST:G3O-31735-MON; -.
DR PRO; PR:P47149; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47149; protein.
DR GO; GO:0000776; C:kinetochore; IDA:SGD.
DR GO; GO:0000818; C:nuclear MIS12/MIND complex; IDA:SGD.
DR GO; GO:0000922; C:spindle pole; EXP:ComplexPortal.
DR GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; IC:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IDA:SGD.
DR InterPro; IPR016851; Nnf1.
DR InterPro; IPR007128; PMF1/Nnf1.
DR PANTHER; PTHR15459; PTHR15459; 1.
DR Pfam; PF03980; Nnf1; 1.
DR PIRSF; PIRSF027153; Nnf1p; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Kinetochore; Mitosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..201
FT /note="Kinetochore-associated protein NNF1"
FT /id="PRO_0000096885"
FT CONFLICT 55
FT /note="R -> S (in Ref. 1; CAA63725)"
FT /evidence="ECO:0000305"
FT CONFLICT 89
FT /note="I -> M (in Ref. 1; CAA63725)"
FT /evidence="ECO:0000305"
FT CONFLICT 152
FT /note="L -> F (in Ref. 1; CAA63725)"
FT /evidence="ECO:0000305"
FT CONFLICT 164..165
FT /note="NE -> KQ (in Ref. 1; CAA63725)"
FT /evidence="ECO:0000305"
FT CONFLICT 193
FT /note="M -> K (in Ref. 1; CAA63725)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 201 AA; 23640 MW; 53568A8E6EA95694 CRC64;
MVNSHGIRYI RLKQVFNRAL DQSISKLQSW DKVSSCFPQY VNSKQGAINV ANCQRQLTEF
WTELCQREFK EIMEERNVEQ KLNELDELIL EAKERYTDRD QDEVNKGPAI DELSSKELVE
CHLYSQRMHA IHEIDERLAK VNEMNDQLAQ ELKDLETQVE VEKNEIGKMY DEYLGSHTDQ
PANVLLVQSL NDMVLELKEN Y
