NNF2_YEAST
ID NNF2_YEAST Reviewed; 936 AA.
AC P53253; D6VUM1;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Protein NNF2;
GN Name=NNF2; OrderedLocusNames=YGR089W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-10, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=SUB592;
RX PubMed=12872131; DOI=10.1038/nbt849;
RA Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G.,
RA Roelofs J., Finley D., Gygi S.P.;
RT "A proteomics approach to understanding protein ubiquitination.";
RL Nat. Biotechnol. 21:921-926(2003).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- INTERACTION:
CC P53253; P32908: SMC1; NbExp=4; IntAct=EBI-23229, EBI-17402;
CC P53253; P32618: YEL043W; NbExp=3; IntAct=EBI-23229, EBI-22354;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 996 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z72874; CAA97092.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08182.1; -; Genomic_DNA.
DR PIR; S64384; S64384.
DR RefSeq; NP_011603.1; NM_001181218.1.
DR AlphaFoldDB; P53253; -.
DR SMR; P53253; -.
DR BioGRID; 33331; 282.
DR DIP; DIP-2984N; -.
DR IntAct; P53253; 14.
DR MINT; P53253; -.
DR STRING; 4932.YGR089W; -.
DR iPTMnet; P53253; -.
DR MaxQB; P53253; -.
DR PaxDb; P53253; -.
DR PRIDE; P53253; -.
DR EnsemblFungi; YGR089W_mRNA; YGR089W; YGR089W.
DR GeneID; 852980; -.
DR KEGG; sce:YGR089W; -.
DR SGD; S000003321; NNF2.
DR VEuPathDB; FungiDB:YGR089W; -.
DR eggNOG; ENOG502QSPS; Eukaryota.
DR HOGENOM; CLU_013934_0_0_1; -.
DR InParanoid; P53253; -.
DR OMA; CSYDIQF; -.
DR BioCyc; YEAST:G3O-30799-MON; -.
DR PRO; PR:P53253; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53253; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Isopeptide bond; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Ubl conjugation.
FT CHAIN 1..936
FT /note="Protein NNF2"
FT /id="PRO_0000202809"
FT TOPO_DOM 1..41
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..120
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..245
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..936
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 297..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 387..437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 512..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 512..528
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 10
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000269|PubMed:12872131"
SQ SEQUENCE 936 AA; 106466 MW; C1F7D631DE73D4D1 CRC64;
MEEQFTNQKK VSHLQSLMNT KRSEQPTEFA KKHRFKDTLA LFLVFLSFNH FTSLCLLVSF
IVATKCKDFL ANCFIILFLS KKPSRHIGEV AHIDISTSKV TNGSSNRKSN SRFFGNSKNS
FVIPIPVLIC EILFAMLLKI YGGDYFVKPI KNLAISIIAS FLINDPSDCL SYATSCSVLY
AVSTNTFQRV SHFFDIIQLF DMSLRGTGQS IKLFTVFRKY SQFFKKLFSL FLPMPFKMLG
KHSDSMIYYL SFHILFFSFA SSLLHPHRQT AENKPLKKGF NSTKPDVARV QGLQKMRISS
SSSVSADSNT LEDQSPMIPN DPGGLSSSNQ TIHPSQQNNS PVPLSSHSNI LNPAASYPTD
ATSSFPYFTS MVKEYKSYQP SVISAEGSNS QAVTTTTSTT TSPTTFNFSG DNTSLSNEIS
LSDSSNGNSK KDSDFFSPSN DKYTNQLFEL NVDFGNMFSS SKLSSDISVT SNLENFIRLL
FRRKNQHLIA PLWSMVVTLK TTNFEKKYLQ ETSENSLTPT NSNTSYVSNQ EKHDKDLDTI
NTHSVSSRIS FTHAGKFKKS VFNNFEPSNT MALIAKTTSD DYNLLNLVST NENIFNRNDN
DYKVCIIDIS TNSITFHIEN LHDGELIVLV NGVIWSEVSC ALILEHVGEE YVVVNGLVPS
CSYDIQFINR LNHRDDYLVS DLIVRTCGNN NAIAGKFENL DFSFPSYYHR KFLSPLLTLK
HSVLTTNANL SDERTKLKKT KKEFSKKLSL LRQEIDYFKG RISQNATHDE KSTLKVENLK
VALQQSETAV NKLEMQLKTL TEKELELEEE YLKKKDLHLK NQLEFSKLEE SLSKDLKNSE
GRFQKVNQEL VQLGSKLDKL NARNEKLQKE VDQNAEEIEK FSTQFLSKRE KDRFRRKEYR
IREANKFELT IKGLEQDINR LENENENIHS LIGNSY
