ID   NO12B_MEDSA             Reviewed;         113 AA.
AC   Q40339; Q40342;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Early nodulin-12B;
DE            Short=N-12B;
DE   Flags: Precursor;
GN   Name=ENOD12B;
OS   Medicago sativa (Alfalfa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Nagyszenasi;
RX   PubMed=7678770; DOI=10.1007/bf00019952;
RA   Allison L.A., Kiss G.B., Bauer P., Poiret M., Pierre M., Savoure A.,
RA   Kondorosi E., Kondorosi A.;
RT   "Identification of two alfalfa early nodulin genes with homology to members
RT   of the pea Enod12 gene family.";
RL   Plant Mol. Biol. 21:375-380(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 15-113.
RC   STRAIN=cv. Coerula W2;
RX   PubMed=8148645; DOI=10.2307/3869639;
RA   Csanadi G., Szecsi J., Kalo P., Kiss P., Endre G., Kondorosi A.,
RA   Kondorosi E., Kiss G.B.;
RT   "ENOD12, an early nodulin gene, is not required for nodule formation and
RT   efficient nitrogen fixation in alfalfa.";
RL   Plant Cell 6:201-213(1994).
CC   -!- FUNCTION: Involved in the infection process during the plant-rhizobium
CC       interaction. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed only in young nodules.
CC   -!- SIMILARITY: Belongs to the plant proline-rich protein superfamily.
CC       ENOD12 family. {ECO:0000305}.
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DR   EMBL; X67507; CAA47844.1; -; Genomic_DNA.
DR   EMBL; X74355; CAA52397.1; -; Genomic_DNA.
DR   PIR; S31076; S31076.
DR   AlphaFoldDB; Q40339; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Cell wall; Nodulation; Repeat; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..113
FT                   /note="Early nodulin-12B"
FT                   /id="PRO_0000019802"
FT   REPEAT          34..38
FT                   /note="1"
FT   REPEAT          39..43
FT                   /note="2"
FT   REPEAT          44..48
FT                   /note="3; approximate"
FT   REPEAT          49..53
FT                   /note="4"
FT   REPEAT          54..58
FT                   /note="5"
FT   REPEAT          59..63
FT                   /note="6"
FT   REPEAT          64..68
FT                   /note="7; approximate"
FT   REPEAT          69..73
FT                   /note="8"
FT   REPEAT          74..78
FT                   /note="9"
FT   REPEAT          79..83
FT                   /note="10"
FT   REPEAT          84..88
FT                   /note="11"
FT   REPEAT          89..93
FT                   /note="12; approximate"
FT   REPEAT          94..98
FT                   /note="13"
FT   REPEAT          99..103
FT                   /note="14"
FT   REPEAT          105..109
FT                   /note="15"
FT   REGION          29..113
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          34..109
FT                   /note="15 X 5 AA approximate tandem repeats of P-P-
FT                   [QVHRTA]-[NHKE]-[KEDT]"
FT   COMPBIAS        45..113
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         87
FT                   /note="K -> R (in strain: cv. Coerula W2)"
FT   VARIANT         107
FT                   /note="A -> S (in strain: cv. Coerula W2)"
SQ   SEQUENCE   113 AA;  12849 MW;  7888B9C46B4608A5 CRC64;
     MASFSLSILV FFISALVLVP QGFAEYYLNP AYRPPQTKPP VNKPSHKEPP VHKPPHKEPP
     VNKPRHKEPP VHKPPHKDPP VNKPPQKESP VHKPPRKEPP THKHPPAEDN IHF