ID   NO12_VICSA              Reviewed;         100 AA.
AC   Q41701;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Early nodulin-12;
DE            Short=N-12;
DE   Flags: Precursor;
GN   Name=ENOD12;
OS   Vicia sativa (Spring vetch) (Tare).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Vicia.
OX   NCBI_TaxID=3908;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nigra; TISSUE=Root nodule;
RX   PubMed=7548828; DOI=10.1007/bf00032671;
RA   Vijn I., Yang W.-C., Pallisgaard N., Oestergaard Jensen E., van Kammen A.,
RA   Bisseling T.;
RT   "VsENOD5, VsENOD12 and VsENOD40 expression during Rhizobium-induced nodule
RT   formation on Vicia sativa roots.";
RL   Plant Mol. Biol. 28:1111-1119(1995).
CC   -!- FUNCTION: Involved in the infection process during the plant-rhizobium
CC       interaction. May also have an additional function in the proliferation
CC       of the bacteria.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during rhizobium-induced nodule
CC       formation. In 4-day old nodules it is found in all the cells of the
CC       center of the nodule primordium and also occurs in the root cortical
CC       cells containing the infection thread. At day 5, expression is seen in
CC       the complete central tissue and at day 20, expressed in the complete
CC       prefixation zone II and is present at a similar level throughout the
CC       zone. In the distal part of the prefixation zone II it is probably
CC       expressed in all cells, whereas in the proximal part, found only in the
CC       infected cells. Expression decreases at the transition of prefixation
CC       zone II into interzone II-III.
CC   -!- SIMILARITY: Belongs to the plant proline-rich protein superfamily.
CC       ENOD12 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA58653.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X83682; CAA58653.1; ALT_INIT; mRNA.
DR   PIR; S60045; S60045.
DR   AlphaFoldDB; Q41701; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Cell wall; Nodulation; Repeat; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..100
FT                   /note="Early nodulin-12"
FT                   /id="PRO_0000019806"
FT   REPEAT          39..43
FT                   /note="1"
FT   REPEAT          44..48
FT                   /note="2"
FT   REPEAT          49..53
FT                   /note="3; approximate"
FT   REPEAT          54..58
FT                   /note="4"
FT   REPEAT          59..63
FT                   /note="5"
FT   REPEAT          64..68
FT                   /note="6; approximate"
FT   REPEAT          69..73
FT                   /note="7"
FT   REPEAT          74..78
FT                   /note="8"
FT   REGION          32..100
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          39..78
FT                   /note="8 X 5 AA approximate tandem repeats of P-P-[VQHR]-
FT                   [NKH]-[EK]"
FT   COMPBIAS        37..58
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        69..86
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   100 AA;  11297 MW;  BBD5498CC5D7D9F1 CRC64;
     MASFLLSTLV FFLAALILVP QGLAQYHLNP VYEAPVNGPP VNKPPQKETP VQKPPQKEPP
     VHKSPRNEPP RHKPPHKKSH LHVTKRSYGK HATEEHSIHF