ID   NO25_MEDSA              Reviewed;         246 AA.
AC   P10322;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Nodulin-25;
DE            Short=N-25;
DE   Flags: Precursor;
GN   Name=NMS-25;
OS   Medicago sativa (Alfalfa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Cardinal;
RX   PubMed=2102828; DOI=10.1007/bf00027493;
RA   Kiss G.B., Vincze E., Vegh Z., Toth G., Soos J.;
RT   "Identification and cDNA cloning of a new nodule-specific gene, Nms-25
RT   (nodulin-25) of Medicago sativa.";
RL   Plant Mol. Biol. 14:467-475(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Nagyszenasi;
RX   PubMed=1966488; DOI=10.1007/bf00036915;
RA   Vegh Z., Vincze E., Kadirov R., Toth G., Kiss G.B.;
RT   "The nucleotide sequence of a nodule-specific gene, Nms-25 of Medicago
RT   sativa: its primary evolution via exon-shuffling and retrotransposon-
RT   mediated DNA rearrangements.";
RL   Plant Mol. Biol. 15:295-306(1990).
CC   -!- FUNCTION: Involved in the development and function of nodules. It might
CC       participate in the biological process of symbiotic nitrogen fixation.
CC   -!- SUBCELLULAR LOCATION: Symbiosome, peribacteroid space.
CC   -!- MISCELLANEOUS: The sequence shown is that of cv. Nagyszenasi.
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DR   EMBL; X13289; CAA31646.1; -; mRNA.
DR   EMBL; X13288; CAA31645.1; -; mRNA.
DR   EMBL; X13287; CAA31644.1; -; Genomic_DNA.
DR   PIR; S11873; ZZAACD.
DR   PIR; S14710; ZZAAGY.
DR   AlphaFoldDB; P10322; -.
DR   GO; GO:0043662; C:peribacteroid fluid; IEA:UniProtKB-SubCell.
DR   GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Nitrogen fixation; Nodulation; Signal.
FT   SIGNAL          1..24
FT   CHAIN           25..246
FT                   /note="Nodulin-25"
FT                   /id="PRO_0000019798"
FT   VARIANT         76
FT                   /note="M -> I (in strain: cv. Cardinal; clone 910)"
FT   VARIANT         97
FT                   /note="L -> P (in strain: cv. Cardinal; clone 910)"
FT   VARIANT         146
FT                   /note="R -> G (in strain: cv. Cardinal; clone 910)"
FT   VARIANT         150
FT                   /note="D -> E (in strain: cv. Cardinal; clone 910)"
FT   VARIANT         190
FT                   /note="S -> P (in strain: cv. Cardinal; clone 386)"
FT   VARIANT         195
FT                   /note="S -> P (in strain: cv. Cardinal; clones 386 and
FT                   910)"
FT   VARIANT         223
FT                   /note="P -> L (in strain: cv. Cardinal; clone 386)"
FT   VARIANT         245
FT                   /note="I -> N (in strain: cv. Cardinal; clone 910)"
FT   VARIANT         245
FT                   /note="I -> S (in strain: cv. Cardinal; clone 386)"
SQ   SEQUENCE   246 AA;  27529 MW;  FB5A07F1A630F727 CRC64;
     MVYSNTYMLL GLGVFVLLSS HVLAYNMRAD PSIHDSTLDD QKSNDFVKTA SIAECPPGPD
     TYLELSDSTL DDQKSMDYVK DASTVDHNCP PGPHTYLELS AWLQEEQNSI DKVKKVLRTD
     EAPDNQKGLE YKDARHNDGP IEISTRTEKD NFIPSDGPIE ISTRTENENF IPSDEPIEIS
     TGTEKENFIS SDEPSEISSG AEKENFIPSV SQVDWRTFHA KYPWIKKDGP NTVTGSRKLL
     NDIAIK