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NO66_BRAFL
ID   NO66_BRAFL              Reviewed;         607 AA.
AC   C3XRY1;
DT   19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66;
DE            EC=1.14.11.-;
DE            EC=1.14.11.27;
DE   AltName: Full=Histone lysine demethylase NO66;
GN   ORFNames=BRAFLDRAFT_123918;
OS   Branchiostoma floridae (Florida lancelet) (Amphioxus).
OC   Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC   Branchiostomidae; Branchiostoma.
OX   NCBI_TaxID=7739;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S238N-H82; TISSUE=Testis;
RX   PubMed=18563158; DOI=10.1038/nature06967;
RA   Putnam N.H., Butts T., Ferrier D.E.K., Furlong R.F., Hellsten U.,
RA   Kawashima T., Robinson-Rechavi M., Shoguchi E., Terry A., Yu J.-K.,
RA   Benito-Gutierrez E.L., Dubchak I., Garcia-Fernandez J., Gibson-Brown J.J.,
RA   Grigoriev I.V., Horton A.C., de Jong P.J., Jurka J., Kapitonov V.V.,
RA   Kohara Y., Kuroki Y., Lindquist E., Lucas S., Osoegawa K., Pennacchio L.A.,
RA   Salamov A.A., Satou Y., Sauka-Spengler T., Schmutz J., Shin-I T.,
RA   Toyoda A., Bronner-Fraser M., Fujiyama A., Holland L.Z., Holland P.W.H.,
RA   Satoh N., Rokhsar D.S.;
RT   "The amphioxus genome and the evolution of the chordate karyotype.";
RL   Nature 453:1064-1071(2008).
CC   -!- FUNCTION: Oxygenase that can act as both a histone lysine demethylase
CC       and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-
CC       4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a
CC       central role in histone code. Also catalyzes the hydroxylation of 60S
CC       ribosomal protein L8 (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + L-histidyl-[protein] + O2 = (3S)-3-hydroxy-L-
CC         histidyl-[protein] + CO2 + succinate; Xref=Rhea:RHEA:54256,
CC         Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:13840, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:29979,
CC         ChEBI:CHEBI:30031, ChEBI:CHEBI:138021;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 2-oxoglutarate + N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3]
CC         + 2 O2 = 2 CO2 + 2 formaldehyde + L-lysyl(36)-[histone H3] + 2
CC         succinate; Xref=Rhea:RHEA:42032, Rhea:RHEA-COMP:9785, Rhea:RHEA-
CC         COMP:9787, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810,
CC         ChEBI:CHEBI:16842, ChEBI:CHEBI:29969, ChEBI:CHEBI:30031,
CC         ChEBI:CHEBI:61976; EC=1.14.11.27;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ROX family. NO66 subfamily. {ECO:0000305}.
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DR   EMBL; GG666456; EEN69449.1; -; Genomic_DNA.
DR   RefSeq; XP_002613440.1; XM_002613394.1.
DR   AlphaFoldDB; C3XRY1; -.
DR   SMR; C3XRY1; -.
DR   STRING; 7739.XP_002613440.1; -.
DR   eggNOG; KOG3706; Eukaryota.
DR   InParanoid; C3XRY1; -.
DR   OrthoDB; 667941at2759; -.
DR   Proteomes; UP000001554; Partially assembled WGS sequence.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0016706; F:2-oxoglutarate-dependent dioxygenase activity; ISS:UniProtKB.
DR   GO; GO:0140680; F:histone H3-di/monomethyl-lysine-36 demethylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051864; F:histone H3-methyl-lysine-36 demethylase activity; ISS:UniProtKB.
DR   GO; GO:0032453; F:histone H3-methyl-lysine-4 demethylase activity; IBA:GO_Central.
DR   GO; GO:0034647; F:histone H3-tri/di/monomethyl-lysine-4 demethylase activity; ISS:UniProtKB.
DR   GO; GO:0005506; F:iron ion binding; ISS:UniProtKB.
DR   GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR   GO; GO:0070544; P:histone H3-K36 demethylation; ISS:UniProtKB.
DR   GO; GO:0034720; P:histone H3-K4 demethylation; ISS:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   InterPro; IPR003347; JmjC_dom.
DR   InterPro; IPR039994; JmjC_protein.
DR   InterPro; IPR013109; NO66.
DR   PANTHER; PTHR13096; PTHR13096; 1.
DR   PANTHER; PTHR13096:SF4; PTHR13096:SF4; 1.
DR   Pfam; PF08007; Cupin_4; 2.
DR   PROSITE; PS51184; JMJC; 1.
PE   3: Inferred from homology;
KW   Chromatin regulator; Dioxygenase; Iron; Metal-binding; Nucleus;
KW   Oxidoreductase; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..607
FT                   /note="Bifunctional lysine-specific demethylase and
FT                   histidyl-hydroxylase NO66"
FT                   /id="PRO_0000390978"
FT   DOMAIN          188..405
FT                   /note="JmjC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00538"
FT   REGION          23..121
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          139..184
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        23..39
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        40..58
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        98..121
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        139..164
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         328
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00538"
FT   BINDING         330
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00538"
FT   BINDING         371
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00538"
SQ   SEQUENCE   607 AA;  68105 MW;  C57DC29A79D0F834 CRC64;
     MAQNIPTKRQ SAFGVFVKSK VKGPTIQQTG ATKTPKTPSK IRRLSIRKST RKIKHALKGK
     SGSQPVAAHK DMPVTQNTGQ GEEGVKLKGY KMKTSPARVQ EPQRTGSPEE NSIGKGNIKT
     NIKGSQNVQA GQKFQERIAT QHPQKRKPFG IEDSDDKTPV KRVRSDTAKS VQSPAKAVDV
     GEVEDSTEEA EKMFEWLIHP VKKEKFFSEL WEKKPLLVKR HLESYNDGWF STEDLTKILH
     ENDIQFGRNL DVTTYEGGQR ETHNPPGRAN PAVVWDYYQN GCSVRLLNPQ TYSQGVWRLC
     STLQEYFSSM VGANIYLTPP GTQGFAPHYD DIEAFVLQLE GNFSQEEIGE AILDVTLEPG
     DLLYFPRGTI HQASALPDTH SLHITVSTCQ RNTWGDLMEK LVPAALTMAF SEDVEFRQAL
     PRDYLDYMGL ANADLDDPRR KAFLETLQSL LSRLVNYVPV DAGVDQKAVE FMRDCLPPVF
     TKNERACSIY GCRTRLEKGR VVGSVDLKTS TPVKLIRKGA ARLVMEGEQV FLYHVLENAR
     VYHGAELQPI EVPPEAAPAI EYLMHSYPEY VTVDSFPLDH QQDKIDLAML MFEKGIIIAQ
     EPASVDN
 
 
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