NOB1_CAEEL
ID NOB1_CAEEL Reviewed; 243 AA.
AC G5EFY5; G5EGL1; Q7K756; Q7KKJ1;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2017, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Homeobox protein nob-1 {ECO:0000305};
GN Name=nob-1 {ECO:0000312|WormBase:Y75B8A.2a};
GN ORFNames=Y75B8A.2 {ECO:0000312|WormBase:Y75B8A.2a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAD48874.1,
RC ECO:0000312|EMBL:AAD48875.1};
RX PubMed=10781051; DOI=10.1073/pnas.97.9.4499;
RA Van Auken K., Weaver D.C., Edgar L.G., Wood W.B.;
RT "Caenorhabditis elegans embryonic axial patterning requires two recently
RT discovered posterior-group Hox genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:4499-4503(2000).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, AND INTERACTION WITH NHR-25.
RX PubMed=15314147; DOI=10.1128/mcb.24.17.7345-7358.2004;
RA Chen Z., Eastburn D.J., Han M.;
RT "The Caenorhabditis elegans nuclear receptor gene nhr-25 regulates
RT epidermal cell development.";
RL Mol. Cell. Biol. 24:7345-7358(2004).
RN [4] {ECO:0000305}
RP INTERACTION WITH GMN-1.
RX PubMed=15811859; DOI=10.1074/jbc.c500070200;
RA Yanagi K., Mizuno T., Tsuyama T., Tada S., Iida Y., Sugimoto A., Eki T.,
RA Enomoto T., Hanaoka F.;
RT "Caenorhabditis elegans geminin homologue participates in cell cycle
RT regulation and germ line development.";
RL J. Biol. Chem. 280:19689-19694(2005).
RN [5] {ECO:0000305}
RP FUNCTION, AND INTERACTION WITH CEH-20.
RX PubMed=16824957; DOI=10.1016/j.devcel.2006.04.020;
RA Arata Y., Kouike H., Zhang Y., Herman M.A., Okano H., Sawa H.;
RT "Wnt signaling and a Hox protein cooperatively regulate psa-3/Meis to
RT determine daughter cell fate after asymmetric cell division in C.
RT elegans.";
RL Dev. Cell 11:105-115(2006).
RN [6] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY MIR-57 (ISOFORM B), AND
RP DISRUPTION PHENOTYPE.
RX PubMed=20824072; DOI=10.1371/journal.pgen.1001089;
RA Zhao Z., Boyle T.J., Liu Z., Murray J.I., Wood W.B., Waterston R.H.;
RT "A negative regulatory loop between microRNA and Hox gene controls
RT posterior identities in Caenorhabditis elegans.";
RL PLoS Genet. 6:e1001089-e1001089(2010).
RN [7] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=21408209; DOI=10.1371/journal.pgen.1002010;
RA Nelson M.D., Zhou E., Kiontke K., Fradin H., Maldonado G., Martin D.,
RA Shah K., Fitch D.H.;
RT "A bow-tie genetic architecture for morphogenesis suggested by a genome-
RT wide RNAi screen in Caenorhabditis elegans.";
RL PLoS Genet. 7:E1002010-E1002010(2011).
RN [8] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=27472063; DOI=10.1038/cdd.2016.77;
RA Malin J.A., Kinet M.J., Abraham M.C., Blum E.S., Shaham S.;
RT "Transcriptional control of non-apoptotic developmental cell death in C.
RT elegans.";
RL Cell Death Differ. 23:1985-1994(2016).
CC -!- FUNCTION: Transcription factor, involved in posterior embryonic
CC patterning, morphogenetic movements of the posterior hypodermis, and
CC cell fate specification (PubMed:21408209, PubMed:10781051,
CC PubMed:16824957, PubMed:20824072). Binds to the 5'-TAGT-3' motif in
CC regulatory elements of genes, including Meis protein psa-3 and microRNA
CC mir-57 (PubMed:16824957, PubMed:20824072). Involved in a negative
CC regulatory loop with mir-57 to specify posterior cell identities
CC (PubMed:20824072). Required for asymmetric division of the T hypodermal
CC cell, acting via the regulation of asymmetric expression of psa-3 in
CC cooperation with ceh-20 and the Wnt-MAPK pathway (PubMed:16824957).
CC Involved in the regulation of the onset of non-apoptotic cell death in
CC the linker cell, acting together with the Wnt signaling pathway
CC (PubMed:27472063). Involved in promoting embryogenesis, in concert with
CC orphan nuclear receptor nhr-25 (PubMed:15314147). May regulate
CC expression of transcription factor dmd-3 (PubMed:21408209).
CC {ECO:0000269|PubMed:10781051, ECO:0000269|PubMed:15314147,
CC ECO:0000269|PubMed:16824957, ECO:0000269|PubMed:20824072,
CC ECO:0000269|PubMed:21408209, ECO:0000269|PubMed:27472063}.
CC -!- SUBUNIT: Interacts with nuclear receptor nhr-25 (PubMed:15314147).
CC Interacts with geminin homolog gmn-1 (PubMed:15811859). Interacts with
CC homeodomain protein ceh-20 (PubMed:16824957).
CC {ECO:0000269|PubMed:15314147, ECO:0000269|PubMed:15811859,
CC ECO:0000269|PubMed:16824957}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|RuleBase:RU000682}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:Y75B8A.2a};
CC IsoId=G5EFY5-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:Y75B8A.2b};
CC IsoId=G5EFY5-2; Sequence=VSP_061102;
CC -!- DEVELOPMENTAL STAGE: Expressed from the 100-cell stage in posterior
CC progeny of ABp(l/r)pp and ABp(l/r)ap (PubMed:20824072). Expressed in
CC the tail tip cells hyp8-11 throughout larval development in both sexes
CC (PubMed:21408209). Expressed in the linker cell from the L3 larval
CC stage onward (PubMed:27472063). {ECO:0000269|PubMed:20824072,
CC ECO:0000269|PubMed:21408209, ECO:0000269|PubMed:27472063}.
CC -!- INDUCTION: [Isoform b]: Repressed directly by microRNA mir-57.
CC {ECO:0000269|PubMed:20824072}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown applied to adults causes
CC posterior morphological abnormalities in their F1 progeny
CC (PubMed:10781051). Knockdown by feeding of dsRNA to stage L1 larvae
CC causes defects in tail tip morphogenesis and reduces expression of dmd-
CC 3; both of these phenotypes are exacerbated on a php-3 mutant
CC background (PubMed:21408209). Knockdown represses expression of
CC microRNA mir-57 in the AB cell sublineages (PubMed:20824072).
CC {ECO:0000269|PubMed:10781051, ECO:0000269|PubMed:20824072,
CC ECO:0000269|PubMed:21408209}.
CC -!- SIMILARITY: Belongs to the abd-b homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD48875.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AF172090; AAD48874.1; -; mRNA.
DR EMBL; AF172091; AAD48875.1; ALT_SEQ; mRNA.
DR EMBL; BX284603; CAA22093.3; -; Genomic_DNA.
DR EMBL; BX284603; CAC70124.2; -; Genomic_DNA.
DR PIR; T27393; T27393.
DR RefSeq; NP_001022941.1; NM_001027770.2.
DR RefSeq; NP_499575.3; NM_067174.4.
DR AlphaFoldDB; G5EFY5; -.
DR SMR; G5EFY5; -.
DR IntAct; G5EFY5; 1.
DR STRING; 6239.Y75B8A.2a; -.
DR PaxDb; G5EFY5; -.
DR EnsemblMetazoa; Y75B8A.2a.1; Y75B8A.2a.1; WBGene00003779. [G5EFY5-1]
DR EnsemblMetazoa; Y75B8A.2b.1; Y75B8A.2b.1; WBGene00003779. [G5EFY5-2]
DR WormBase; Y75B8A.2a; CE51986; WBGene00003779; nob-1.
DR WormBase; Y75B8A.2b; CE52032; WBGene00003779; nob-1.
DR eggNOG; KOG0487; Eukaryota.
DR HOGENOM; CLU_1190784_0_0_1; -.
DR InParanoid; G5EFY5; -.
DR OrthoDB; 976319at2759; -.
DR PhylomeDB; G5EFY5; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00003779; Expressed in embryo and 3 other tissues.
DR ExpressionAtlas; G5EFY5; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0009887; P:animal organ morphogenesis; IBA:GO_Central.
DR GO; GO:0009948; P:anterior/posterior axis specification; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:WormBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0010172; P:embryonic body morphogenesis; IMP:WormBase.
DR GO; GO:0009880; P:embryonic pattern specification; IMP:WormBase.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:WormBase.
DR GO; GO:1903800; P:positive regulation of miRNA maturation; IMP:UniProtKB.
DR GO; GO:0110039; P:positive regulation of nematode male tail tip morphogenesis; IMP:UniProtKB.
DR GO; GO:0012501; P:programmed cell death; IMP:UniProtKB.
DR GO; GO:0009786; P:regulation of asymmetric cell division; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..243
FT /note="Homeobox protein nob-1"
FT /id="PRO_0000453033"
FT DNA_BIND 162..221
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 40..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..151
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_061102"
FT CONFLICT 108..132
FT /note="Missing (in Ref. 1; AAD48874)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 243 AA; 27178 MW; 846C15EA414A0656 CRC64;
MISVMQQMIN NDSPEDSKES ITSVQQTPFF WPSAAAAIPS IQGESRSERE SETGSSPQLA
PSSTGMVMPG TAGMYGFGPS RMPTANEFGM MMNPVYTDFY QNPLASTGWY SYGQPYQFTA
NYSIPSLDGN LSDITIPTTA GSSAATTPNA AMHLPWAISH DGKKKRQPYK KDQISRLEYE
YSVNQYLTNK RRSELSAQLM LDEKQVKVWF QNRRMKDKKL RQRHSGPFPH GAPVTPCIER
LIN
