NOB1_METJA
ID NOB1_METJA Reviewed; 197 AA.
AC Q58869;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Endoribonuclease Nob1;
DE Short=RNase Nob1;
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE AltName: Full=Endonuclease VapC5;
DE AltName: Full=Putative toxin VapC5 {ECO:0000255|HAMAP-Rule:MF_00265};
GN Name=nob1; Synonyms=vapC5; OrderedLocusNames=MJ1474;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP POSSIBLE FUNCTION.
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=15718296; DOI=10.1093/nar/gki201;
RA Pandey D.P., Gerdes K.;
RT "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT host-associated prokaryotes.";
RL Nucleic Acids Res. 33:966-976(2005).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC Processes pre-16S-rRNA at its 3' end (the D-site) to yield the mature
CC 3' end (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000305};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- DOMAIN: Has 2 structurally independent domains; the N-terminal PINc
CC domain which binds Mn(2+), rRNA substrate and probably has
CC endoribonuclease activity, and the C-terminal zinc ribbon domain which
CC also binds rRNA substrate. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR EMBL; L77117; AAB99480.1; -; Genomic_DNA.
DR PIR; A64484; A64484.
DR AlphaFoldDB; Q58869; -.
DR SMR; Q58869; -.
DR STRING; 243232.MJ_1474; -.
DR EnsemblBacteria; AAB99480; AAB99480; MJ_1474.
DR KEGG; mja:MJ_1474; -.
DR eggNOG; arCOG00721; Archaea.
DR HOGENOM; CLU_109674_0_0_2; -.
DR InParanoid; Q58869; -.
DR OMA; GCGREFD; -.
DR PhylomeDB; Q58869; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IBA:GO_Central.
DR GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IBA:GO_Central.
DR CDD; cd09876; PIN_Nob1-like; 1.
DR HAMAP; MF_00265; VapC_Nob1; 1.
DR InterPro; IPR039907; NOB1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR InterPro; IPR033411; Ribonuclease_PIN.
DR InterPro; IPR022907; VapC_family.
DR PANTHER; PTHR12814; PTHR12814; 2.
DR Pfam; PF17146; PIN_6; 1.
DR SMART; SM00670; PINc; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Manganese; Metal-binding; Nuclease;
KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA-binding;
KW Toxin-antitoxin system; Zinc.
FT CHAIN 1..197
FT /note="Endoribonuclease Nob1"
FT /id="PRO_0000156045"
FT DOMAIN 35..141
FT /note="PINc"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT REGION 147..152
FT /note="Flexible linker"
FT /evidence="ECO:0000250"
FT REGION 153..188
FT /note="Zinc ribbon"
FT /evidence="ECO:0000250"
FT BINDING 37
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255"
FT BINDING 158
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 161
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 174
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 177
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
SQ SEQUENCE 197 AA; 22781 MW; 70075BB626934F58 CRC64;
MLSLPPPEFY KLWIINKSPY QLFNIYRSGI MKVKVLDASA IIHGYNPIIE EGEHYTTPEV
LEEIESKKII VEQALDFGKL KIMSPNREYI KKVEEVVKKT GDNLSQQDIG VLALALNLNA
ILYTDDYGIQ NVAKKLNIEV RGIAFEPTNK DFIWRKICEG CKKLYPVDYE EDICEICGSP
LKRKMVKSRL KKKRKKK
