ID   NOB1_METTH              Reviewed;         171 AA.
AC   O27890;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Endoribonuclease Nob1;
DE            Short=RNase Nob1;
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Endonuclease VapC2;
DE   AltName: Full=Putative toxin VapC2 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=nob1; Synonyms=vapC2; OrderedLocusNames=MTH_1862;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Processes pre-16S-rRNA at its 3' end (the D-site) to yield the mature
CC       3' end (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000305};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- DOMAIN: Has 2 structurally independent domains; the N-terminal PINc
CC       domain which binds Mn(2+), rRNA substrate and probably has
CC       endoribonuclease activity, and the C-terminal zinc ribbon domain which
CC       also binds rRNA substrate. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AE000666; AAB86328.1; -; Genomic_DNA.
DR   PIR; B69116; B69116.
DR   RefSeq; WP_010877464.1; NC_000916.1.
DR   AlphaFoldDB; O27890; -.
DR   SMR; O27890; -.
DR   STRING; 187420.MTH_1862; -.
DR   EnsemblBacteria; AAB86328; AAB86328; MTH_1862.
DR   GeneID; 1470947; -.
DR   KEGG; mth:MTH_1862; -.
DR   PATRIC; fig|187420.15.peg.1816; -.
DR   HOGENOM; CLU_109674_0_0_2; -.
DR   OMA; GCGREFD; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR   CDD; cd09876; PIN_Nob1-like; 1.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR039907; NOB1.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR033411; Ribonuclease_PIN.
DR   InterPro; IPR022907; VapC_family.
DR   PANTHER; PTHR12814; PTHR12814; 2.
DR   Pfam; PF17146; PIN_6; 1.
DR   SMART; SM00670; PINc; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Manganese; Metal-binding; Nuclease;
KW   Reference proteome; Ribosome biogenesis; RNA-binding; rRNA-binding;
KW   Toxin-antitoxin system; Zinc.
FT   CHAIN           1..171
FT                   /note="Endoribonuclease Nob1"
FT                   /id="PRO_0000156046"
FT   DOMAIN          9..114
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   REGION          130..135
FT                   /note="Flexible linker"
FT                   /evidence="ECO:0000250"
FT   REGION          136..171
FT                   /note="Zinc ribbon"
FT                   /evidence="ECO:0000250"
FT   BINDING         14
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255"
FT   BINDING         133
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255"
FT   BINDING         141
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         144
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         157
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         160
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   171 AA;  19633 MW;  079CA4D3F7F15B52 CRC64;
     MHIDNVVFMK KVLDASAFIN GYVPEGRENY TVRSVTEEIR DFRSMMILED ALREGRLKIT
     EPDPESMKVV EDAISESGDI MRLSPTDMEV IGLAVSLRGK DDVTVITDDY TIQNTLKILG
     IGFRSVLTSG IRDTYSWRRV CTGCRRVYPL DYEFEECEIC GSRIVRKRHR N