NOB1_PYRAB
ID NOB1_PYRAB Reviewed; 161 AA.
AC Q9UZ20; G8ZHE2;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Endoribonuclease Nob1;
DE Short=RNase Nob1;
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE AltName: Full=Endonuclease VapC14;
DE AltName: Full=Putative toxin VapC14 {ECO:0000255|HAMAP-Rule:MF_00265};
GN Name=nob1; Synonyms=vapC14; OrderedLocusNames=PYRAB13370; ORFNames=PAB0882;
OS Pyrococcus abyssi (strain GE5 / Orsay).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=272844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic archaeon
RT Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=GE5 / Orsay;
RX PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA Gao J., Wang J.;
RT "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT Pyrococcus furiosus DSM 3638.";
RL Curr. Microbiol. 64:118-129(2012).
RN [3]
RP POSSIBLE FUNCTION.
RC STRAIN=GE5 / Orsay;
RX PubMed=15718296; DOI=10.1093/nar/gki201;
RA Pandey D.P., Gerdes K.;
RT "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT host-associated prokaryotes.";
RL Nucleic Acids Res. 33:966-976(2005).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC Processes pre-16S-rRNA at its 3' end (the D-site) to yield the mature
CC 3' end (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000305};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- DOMAIN: Has 2 structurally independent domains; the N-terminal PINc
CC domain which binds Mn(2+), rRNA substrate and probably has
CC endoribonuclease activity, and the C-terminal zinc ribbon domain which
CC also binds rRNA substrate. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR EMBL; AJ248287; CAB50242.1; -; Genomic_DNA.
DR EMBL; HE613800; CCE70779.1; -; Genomic_DNA.
DR PIR; E75043; E75043.
DR AlphaFoldDB; Q9UZ20; -.
DR SMR; Q9UZ20; -.
DR STRING; 272844.PAB0882; -.
DR EnsemblBacteria; CAB50242; CAB50242; PAB0882.
DR KEGG; pab:PAB0882; -.
DR PATRIC; fig|272844.11.peg.1422; -.
DR eggNOG; arCOG00721; Archaea.
DR HOGENOM; CLU_109674_1_0_2; -.
DR OMA; GCGREFD; -.
DR PhylomeDB; Q9UZ20; -.
DR Proteomes; UP000000810; Chromosome.
DR Proteomes; UP000009139; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR CDD; cd09876; PIN_Nob1-like; 1.
DR HAMAP; MF_00265; VapC_Nob1; 1.
DR InterPro; IPR039907; NOB1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR InterPro; IPR033411; Ribonuclease_PIN.
DR InterPro; IPR022907; VapC_family.
DR PANTHER; PTHR12814; PTHR12814; 2.
DR Pfam; PF17146; PIN_6; 1.
DR SMART; SM00670; PINc; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Manganese; Metal-binding; Nuclease;
KW Ribosome biogenesis; RNA-binding; rRNA-binding; Toxin-antitoxin system;
KW Zinc.
FT CHAIN 1..161
FT /note="Endoribonuclease Nob1"
FT /id="PRO_0000156047"
FT DOMAIN 5..103
FT /note="PINc"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT REGION 118..123
FT /note="Flexible linker"
FT /evidence="ECO:0000250"
FT REGION 124..159
FT /note="Zinc ribbon"
FT /evidence="ECO:0000250"
FT BINDING 10
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255"
FT BINDING 129
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 132
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 145
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 148
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
SQ SEQUENCE 161 AA; 17976 MW; 3D1CB0D38A9903B7 CRC64;
MLLKLKLVLD SSVFIQGLDI EGYTTPKVVD EVKDRESRIL LESLISSGKV KVVEPSKEAL
RAVKNAALKT GEIEELSEAD LEVLALAYEL KAEVFSDDYN VQNVARILGL KFRTLKRGIK
KVIKWQYVCI GCGRKFKEMP PGGICPDCGS PVKLIPRRQR S
