NOBOX_MOUSE
ID NOBOX_MOUSE Reviewed; 527 AA.
AC Q8VIH1; P70367;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Homeobox protein NOBOX;
DE AltName: Full=Homeodomain-containing protein OG-2;
DE AltName: Full=Newborn ovary homeobox protein;
DE AltName: Full=Oocyte-specific homeobox protein;
GN Name=Nobox; Synonyms=Og2x;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=129/SvEv;
RX PubMed=11804785; DOI=10.1016/s0925-4773(01)00620-7;
RA Suzumori N., Yan C., Matzuk M.M., Rajkovic A.;
RT "Nobox is a homeobox-encoding gene preferentially expressed in primordial
RT and growing oocytes.";
RL Mech. Dev. 111:137-141(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 128-326, AND DEVELOPMENTAL STAGE.
RX PubMed=8855241; DOI=10.1073/pnas.93.20.10691;
RA Rovescalli A.C., Asoh S., Nirenberg M.W.;
RT "Cloning and characterization of four murine homeobox genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:10691-10696(1996).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15326356; DOI=10.1126/science.1099755;
RA Rajkovic A., Pangas S.A., Ballow D., Suzumori N., Matzuk M.M.;
RT "NOBOX deficiency disrupts early folliculogenesis and oocyte-specific gene
RT expression.";
RL Science 305:1157-1159(2004).
RN [5]
RP FUNCTION, DNA-BINDING, AND MUTAGENESIS OF ASN-186.
RX PubMed=16997917; DOI=10.1074/jbc.m604008200;
RA Choi Y., Rajkovic A.;
RT "Characterization of NOBOX DNA binding specificity and its regulation of
RT Gdf9 and Pou5f1 promoters.";
RL J. Biol. Chem. 281:35747-35756(2006).
RN [6]
RP MUTAGENESIS OF ARG-187 AND ARG-192.
RX PubMed=17701902; DOI=10.1086/519496;
RA Qin Y., Choi Y., Zhao H., Simpson J.L., Chen Z.-J., Rajkovic A.;
RT "NOBOX homeobox mutation causes premature ovarian failure.";
RL Am. J. Hum. Genet. 81:576-581(2007).
CC -!- FUNCTION: Transcription factor which plays an essential role in
CC postnatal follicle development. Binds preferentially to the DNA
CC sequences 5'-TAATTG-3', 5'-TAGTTG-3' and 5'-TAATTA-3'. Directly
CC regulates the transcription of POU5F1 and GDF9 during early
CC folliculogenesis. {ECO:0000269|PubMed:15326356,
CC ECO:0000269|PubMed:16997917}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Specifically expressed in ovaries and testes. In
CC ovaries, expressed in oocytes from primordial through antral follicles
CC but not in granulosa cells, theca cells and corpora lutea.
CC {ECO:0000269|PubMed:11804785}.
CC -!- DEVELOPMENTAL STAGE: Expressed from 15.5 dpc.
CC {ECO:0000269|PubMed:8855241}.
CC -!- DISRUPTION PHENOTYPE: Male mice are fertile and show no obvious
CC abnormality. Female mice lacking Nobox have normal gross anatomy and
CC histology, but are infertile with atrophic ovaries that lack oocytes at
CC 6 weeks of age. {ECO:0000269|PubMed:15326356}.
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DR EMBL; AY061761; AAL29683.1; -; mRNA.
DR EMBL; BC125280; AAI25281.1; -; mRNA.
DR EMBL; BC125282; AAI25283.1; -; mRNA.
DR EMBL; U65067; AAC52828.1; -; Genomic_DNA.
DR CCDS; CCDS20093.1; -.
DR RefSeq; NP_570939.1; NM_130869.3.
DR AlphaFoldDB; Q8VIH1; -.
DR SMR; Q8VIH1; -.
DR STRING; 10090.ENSMUSP00000031749; -.
DR iPTMnet; Q8VIH1; -.
DR PhosphoSitePlus; Q8VIH1; -.
DR PaxDb; Q8VIH1; -.
DR PRIDE; Q8VIH1; -.
DR Antibodypedia; 32735; 143 antibodies from 19 providers.
DR DNASU; 18291; -.
DR Ensembl; ENSMUST00000031749; ENSMUSP00000031749; ENSMUSG00000029736.
DR GeneID; 18291; -.
DR KEGG; mmu:18291; -.
DR UCSC; uc009bsp.1; mouse.
DR CTD; 135935; -.
DR MGI; MGI:108011; Nobox.
DR VEuPathDB; HostDB:ENSMUSG00000029736; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00650000093445; -.
DR InParanoid; Q8VIH1; -.
DR OMA; QEAGDKP; -.
DR OrthoDB; 729946at2759; -.
DR PhylomeDB; Q8VIH1; -.
DR TreeFam; TF337576; -.
DR BioGRID-ORCS; 18291; 0 hits in 71 CRISPR screens.
DR PRO; PR:Q8VIH1; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8VIH1; protein.
DR Bgee; ENSMUSG00000029736; Expressed in primary oocyte and 10 other tissues.
DR ExpressionAtlas; Q8VIH1; baseline and differential.
DR Genevisible; Q8VIH1; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005667; C:transcription regulator complex; IC:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR GO; GO:0001541; P:ovarian follicle development; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR042988; NOBOX.
DR PANTHER; PTHR47060; PTHR47060; 2.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Homeobox; Nucleus;
KW Oogenesis; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..527
FT /note="Homeobox protein NOBOX"
FT /id="PRO_0000268866"
FT DNA_BIND 136..195
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 488..527
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 227..243
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 488..508
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 509..527
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 186
FT /note="N->Q: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:16997917"
FT MUTAGEN 187
FT /note="R->H: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:17701902"
FT MUTAGEN 192
FT /note="R->Q: No effect on DNA-binding."
FT /evidence="ECO:0000269|PubMed:17701902"
FT CONFLICT 323..326
FT /note="SIAS -> RYGT (in Ref. 3; AAC52828)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 527 AA; 57565 MW; 1B6AF1323F785290 CRC64;
MEPTEKLCKK MQGQEAGDKP RTAALETEGP LQDSALPIQD DQDKQSSLPR ASLGKRPLSK
TSEELMDAGT CRVHKAPTAA ACGPQSEEEG CSPPERKAES LKPSISAVPG QATAGSLNSH
EGDLKKESLE VTCQFRKKTR TLYRSDQLEE LERIFQEDHY PDSDKRHEIS QMVGVTPQRI
MVWFQNRRAK WRKVEKLNEK ETKNGPAAPS ADSSQHRSAP ELLDPMPTDL EPGPVPPENI
LDVFPEPPML LTSEQTLTPF QNNEGAERVA VTPPLLSPPP IRRANLPLPL GPVQTPQVLP
PMRDVPGSDS IYKDKAYVSW GTSIASPPTY SNLEDLGSQD YQASSQLGSF QLSQAPHLPL
FPSLQSQFPY LPPFPYPIPS SMPFLPPEDS LFSFPFGFSG DSSQDYCPGP PPGQILLQPP
AENMGTGPWS GHCLPEPPFP RPHYPQALGQ PLGAEGYFPN LLPTPYALTM SKQSSLGLNG
LLEGTRVETG SSLSKMSDEQ TSSSLEQPAL EEVRDKNKNS HAAGAKE
