NOC4_YEAST
ID NOC4_YEAST Reviewed; 552 AA.
AC Q06512; D6W4E1;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Nucleolar complex protein 4;
DE AltName: Full=U three protein 19;
DE AltName: Full=U3 small nucleolar RNA-associated protein 19;
DE Short=U3 snoRNA-associated protein 19;
GN Name=NOC4; Synonyms=UTP19; OrderedLocusNames=YPR144C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, INTERACTION WITH NOP14, AND SUBCELLULAR LOCATION.
RX PubMed=12446671; DOI=10.1074/jbc.m208898200;
RA Milkereit P., Strauss D., Bassler J., Gadal O., Kuhn H., Schutz S., Gas N.,
RA Lechner J., Hurt E., Tschochner H.;
RT "A Noc complex specifically involved in the formation and nuclear export of
RT ribosomal 40 S subunits.";
RL J. Biol. Chem. 278:4072-4081(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU
RP PROCESSOME, AND SUBCELLULAR LOCATION.
RX PubMed=15590835; DOI=10.1128/ec.3.6.1619-1626.2004;
RA Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S.,
RA Baserga S.J.;
RT "The small-subunit processome is a ribosome assembly intermediate.";
RL Eukaryot. Cell 3:1619-1626(2004).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16159874; DOI=10.1074/jbc.m506916200;
RA Leger-Silvestre I., Caffrey J.M., Dawaliby R., Alvarez-Arias D.A., Gas N.,
RA Bertolone S.J., Gleizes P.E., Ellis S.R.;
RT "Specific role for yeast homologs of the Diamond Blackfan anemia-associated
RT Rps19 protein in ribosome synthesis.";
RL J. Biol. Chem. 280:38177-38185(2005).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA and
CC ribosome assembly. Has a role in the nuclear export of 40S pre-
CC ribosomal subunit to the cytoplasm. Its subcellular location and
CC association with pre-40S subunit are unaffected by RPS19 disruptions,
CC suggesting it acts before the ribosomal protein.
CC {ECO:0000269|PubMed:12446671, ECO:0000269|PubMed:15590835,
CC ECO:0000269|PubMed:16159874}.
CC -!- SUBUNIT: Interacts with NOP14 and MPP10. Interacts with snoRNA U3.
CC Component of the ribosomal small subunit (SSU) processome composed of
CC at least 40 protein subunits and snoRNA U3.
CC {ECO:0000269|PubMed:12446671, ECO:0000269|PubMed:15590835}.
CC -!- INTERACTION:
CC Q06512; Q99207: NOP14; NbExp=7; IntAct=EBI-36459, EBI-35157;
CC Q06512; P42945: UTP10; NbExp=3; IntAct=EBI-36459, EBI-1884;
CC Q06512; Q02354: UTP6; NbExp=4; IntAct=EBI-36459, EBI-22119;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12446671,
CC ECO:0000269|PubMed:15590835, ECO:0000269|PubMed:16159874}.
CC -!- MISCELLANEOUS: Present with 1630 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the CBF/MAK21 family. {ECO:0000305}.
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DR EMBL; U40829; AAB68283.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11557.1; -; Genomic_DNA.
DR PIR; S69032; S69032.
DR RefSeq; NP_015470.1; NM_001184241.1.
DR PDB; 5WLC; EM; 3.80 A; SU=147-513.
DR PDB; 6KE6; EM; 3.40 A; RO=1-552.
DR PDB; 6LQP; EM; 3.20 A; RO=1-552.
DR PDB; 6LQQ; EM; 4.10 A; RO=1-552.
DR PDB; 6LQR; EM; 8.60 A; RO=1-552.
DR PDB; 6LQS; EM; 3.80 A; RO=1-552.
DR PDB; 6LQU; EM; 3.70 A; RO=1-552.
DR PDB; 6LQV; EM; 4.80 A; RO=1-552.
DR PDB; 6ZQA; EM; 4.40 A; US=1-552.
DR PDB; 6ZQB; EM; 3.90 A; US=1-552.
DR PDB; 6ZQC; EM; 3.80 A; US=1-552.
DR PDB; 6ZQD; EM; 3.80 A; US=1-552.
DR PDB; 6ZQE; EM; 7.10 A; US=1-552.
DR PDB; 6ZQF; EM; 4.90 A; US=1-552.
DR PDB; 7AJT; EM; 4.60 A; US=1-552.
DR PDB; 7AJU; EM; 3.80 A; US=1-552.
DR PDB; 7D4I; EM; 4.00 A; RO=1-552.
DR PDB; 7D5S; EM; 4.60 A; RO=1-552.
DR PDB; 7D63; EM; 12.30 A; RO=1-552.
DR PDBsum; 5WLC; -.
DR PDBsum; 6KE6; -.
DR PDBsum; 6LQP; -.
DR PDBsum; 6LQQ; -.
DR PDBsum; 6LQR; -.
DR PDBsum; 6LQS; -.
DR PDBsum; 6LQU; -.
DR PDBsum; 6LQV; -.
DR PDBsum; 6ZQA; -.
DR PDBsum; 6ZQB; -.
DR PDBsum; 6ZQC; -.
DR PDBsum; 6ZQD; -.
DR PDBsum; 6ZQE; -.
DR PDBsum; 6ZQF; -.
DR PDBsum; 7AJT; -.
DR PDBsum; 7AJU; -.
DR PDBsum; 7D4I; -.
DR PDBsum; 7D5S; -.
DR PDBsum; 7D63; -.
DR AlphaFoldDB; Q06512; -.
DR SMR; Q06512; -.
DR BioGRID; 36313; 299.
DR ComplexPortal; CPX-1735; NOP14-NOC4 complex.
DR DIP; DIP-4555N; -.
DR IntAct; Q06512; 46.
DR MINT; Q06512; -.
DR STRING; 4932.YPR144C; -.
DR MaxQB; Q06512; -.
DR PaxDb; Q06512; -.
DR PRIDE; Q06512; -.
DR EnsemblFungi; YPR144C_mRNA; YPR144C; YPR144C.
DR GeneID; 856267; -.
DR KEGG; sce:YPR144C; -.
DR SGD; S000006348; NOC4.
DR VEuPathDB; FungiDB:YPR144C; -.
DR eggNOG; KOG2154; Eukaryota.
DR GeneTree; ENSGT00390000016776; -.
DR HOGENOM; CLU_015945_1_0_1; -.
DR InParanoid; Q06512; -.
DR OMA; FLDWSYD; -.
DR BioCyc; YEAST:G3O-34278-MON; -.
DR Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:Q06512; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q06512; protein.
DR GO; GO:0030686; C:90S preribosome; HDA:SGD.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0030692; C:Noc4p-Nop14p complex; IPI:SGD.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IDA:GO_Central.
DR GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IC:ComplexPortal.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IC:ComplexPortal.
DR GO; GO:0042274; P:ribosomal small subunit biogenesis; IMP:SGD.
DR GO; GO:0006364; P:rRNA processing; IMP:SGD.
DR InterPro; IPR005612; CCAAT-binding_factor.
DR InterPro; IPR027193; Noc4.
DR PANTHER; PTHR12455; PTHR12455; 1.
DR Pfam; PF03914; CBF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Ribonucleoprotein;
KW Ribosome biogenesis; rRNA processing.
FT CHAIN 1..552
FT /note="Nucleolar complex protein 4"
FT /id="PRO_0000173491"
SQ SEQUENCE 552 AA; 63637 MW; A610425CB4C06A3C CRC64;
MVLLISEIKD IAKRLTAAGD RKQYNSIIKL INELVIPENV TQLEEDETEK NLRFLVMSLF
QIFRKLFSRG DLTLPSSKKS TLEKEQFVNW CRKVYEAFKT KLLAIISDIP FETSLGLDSL
DVYLQLAELE STHFASEKGA PFFPNKTFRK LIIALWSSNM GEIEDVKSSG ASENLIIVEF
TEKYYTKFAD IQYYFQSEFN QLLEDPAYQD LLLKNVGKWL ALVNHDKHCS SVDADLEIFV
PNPPQAIENE SKFKSNFEKN WLSLLNGQLS LQQYKSILLI LHKRIIPHFH TPTKLMDFLT
DSYNLQSSNK NAGVVPILAL NGLFELMKRF NLEYPNFYMK LYQIINPDLM HVKYRARFFR
LMDVFLSSTH LSAHLVASFI KKLARLTLES PPSAIVTVIP FIYNLIRKHP NCMIMLHNPA
FISNPFQTPD QVANLKTLKE NYVDPFDVHE SDPELTHALD SSLWELASLM EHYHPNVATL
AKIFAQPFKK LSYNMEDFLD WNYDSLLNAE SSRKLKTLPT LEFEAFTNVF DNEDGDSEAS
SQGNVYLPGV AW
