NOCT_AGRFC
ID NOCT_AGRFC Reviewed; 283 AA.
AC P35120;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Nopaline-binding periplasmic protein;
DE Flags: Precursor;
GN Name=nocT; OrderedLocusNames=Atu6027; ORFNames=AGR_pTi_67;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1732218; DOI=10.1128/jb.174.3.841-849.1992;
RA Zanker H., von Lintig J., Schroeder J.;
RT "Opine transport genes in the octopine (occ) and nopaline (noc) catabolic
RT regions in Ti plasmids of Agrobacterium tumefaciens.";
RL J. Bacteriol. 174:841-849(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: Component of the nopaline active transport system probably
CC consisting of four subunits: Q, M, P and T. This system is also capable
CC of transporting octopine provided that catabolic functions are induced
CC with nopaline.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 3 family.
CC {ECO:0000305}.
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DR EMBL; M77785; AAA50511.1; -; Genomic_DNA.
DR EMBL; AE007871; AAK90985.2; -; Genomic_DNA.
DR PIR; AI3230; AI3230.
DR PIR; H42600; H42600.
DR RefSeq; NP_396544.2; NC_003065.3.
DR RefSeq; WP_010891637.1; NC_003065.3.
DR PDB; 4P0I; X-ray; 1.89 A; A/B=26-283.
DR PDB; 4POW; X-ray; 1.55 A; A/B=26-283.
DR PDB; 4POX; X-ray; 2.29 A; A/B=26-283.
DR PDB; 4PP0; X-ray; 1.57 A; A/B=26-283.
DR PDB; 5ITO; X-ray; 2.35 A; A/B=26-283.
DR PDB; 5ITP; X-ray; 1.85 A; A/B=26-283.
DR PDB; 5OT8; X-ray; 2.35 A; A/B=26-283.
DR PDB; 5OT9; X-ray; 2.45 A; A/B=26-283.
DR PDB; 5OTA; X-ray; 2.10 A; A/B=26-283.
DR PDB; 5OTC; X-ray; 2.20 A; A/B=26-283.
DR PDB; 5OVZ; X-ray; 1.75 A; A/B=26-283.
DR PDBsum; 4P0I; -.
DR PDBsum; 4POW; -.
DR PDBsum; 4POX; -.
DR PDBsum; 4PP0; -.
DR PDBsum; 5ITO; -.
DR PDBsum; 5ITP; -.
DR PDBsum; 5OT8; -.
DR PDBsum; 5OT9; -.
DR PDBsum; 5OTA; -.
DR PDBsum; 5OTC; -.
DR PDBsum; 5OVZ; -.
DR AlphaFoldDB; P35120; -.
DR SMR; P35120; -.
DR TCDB; 3.A.1.3.6; the atp-binding cassette (abc) superfamily.
DR PRIDE; P35120; -.
DR EnsemblBacteria; AAK90985; AAK90985; Atu6027.
DR KEGG; atu:Atu6027; -.
DR PATRIC; fig|176299.10.peg.5234; -.
DR HOGENOM; CLU_019602_18_0_5; -.
DR OMA; KVEFSVM; -.
DR PhylomeDB; P35120; -.
DR BioCyc; AGRO:ATU6027-MON; -.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro.
DR GO; GO:0015276; F:ligand-gated ion channel activity; IEA:InterPro.
DR GO; GO:0071705; P:nitrogen compound transport; IEA:InterPro.
DR InterPro; IPR001320; Iontro_rcpt.
DR InterPro; IPR005768; Lys_Arg_Orn-bd.
DR InterPro; IPR018313; SBP_3_CS.
DR InterPro; IPR001638; Solute-binding_3/MltF_N.
DR Pfam; PF00497; SBP_bac_3; 1.
DR SMART; SM00062; PBPb; 1.
DR SMART; SM00079; PBPe; 1.
DR TIGRFAMs; TIGR01096; 3A0103s03R; 1.
DR PROSITE; PS01039; SBP_BACTERIAL_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Periplasm; Plasmid; Reference proteome;
KW Signal; Transport.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..283
FT /note="Nopaline-binding periplasmic protein"
FT /id="PRO_0000031767"
FT DISULFID 63..70
FT /evidence="ECO:0000250"
FT CONFLICT 78
FT /note="D -> V (in Ref. 1; AAA50511)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="T -> A (in Ref. 1; AAA50511)"
FT /evidence="ECO:0000305"
FT STRAND 30..35
FT /evidence="ECO:0007829|PDB:4POW"
FT TURN 40..42
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 43..45
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 54..66
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 69..74
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 77..79
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 80..85
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 88..92
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 100..103
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 106..108
FT /evidence="ECO:0007829|PDB:4POX"
FT STRAND 117..122
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 126..129
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 135..138
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 144..157
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 158..160
FT /evidence="ECO:0007829|PDB:5OT9"
FT STRAND 162..165
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 169..177
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 183..188
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 189..197
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 202..207
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 208..216
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 218..220
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 223..232
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 233..235
FT /evidence="ECO:0007829|PDB:4POW"
FT STRAND 237..239
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 248..263
FT /evidence="ECO:0007829|PDB:4POW"
FT HELIX 266..275
FT /evidence="ECO:0007829|PDB:4POW"
SQ SEQUENCE 283 AA; 30768 MW; 9DEB598EA32DE42F CRC64;
MKFFNLNALA AVVTGVLLAA GPTQAKDYKS ITIATEGSYA PYNFKDAGGK LIGFDIDLGN
DLCKRMNIEC KFVEQAWDGI IPSLTAGRYD AIMAAMGIQP AREKVIAFSR PYLLTPMTFL
TTADSPLLKT QVAIENLPLD NITPEQKAEL DKFTKIFEGV KFGVQAGTSH EAFMKQMMPS
VQISTYDTID NVVMDLKAGR IDASLASVSF LKPLTDKPDN KDLKMFGPRM TGGLFGKGVG
VGIRKEDADL KALFDKAIDA AIADGTVQKL SQQWFGYDAS PKQ
