ID   NOD3_MEDTR              Reviewed;         268 AA.
AC   P93332;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   02-JUN-2021, entry version 62.
DE   RecName: Full=Bidirectional sugar transporter N3;
DE   AltName: Full=Nodulin 3;
DE            Short=MtN3;
DE            Short=N-3;
GN   Name=N3;
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND INDUCTION DURING
RP   NODULATION.
RC   STRAIN=cv. Jemalong J5; TISSUE=Root nodule;
RX   PubMed=8634476; DOI=10.1094/mpmi-9-0233;
RA   Gamas P., de Carvalho Niebel F., Lescure N., Cullimore J.;
RT   "Use of a subtractive hybridization approach to identify new Medicago
RT   truncatula genes induced during root nodule development.";
RL   Mol. Plant Microbe Interact. 9:233-242(1996).
CC   -!- FUNCTION: Mediates both low-affinity uptake and efflux of sugar across
CC       the plasma membrane. {ECO:0000250|UniProtKB:Q8L9J7}.
CC   -!- SUBUNIT: Forms homooligomers and/or heterooligomers.
CC       {ECO:0000250|UniProtKB:Q8L9J7}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8L9J7};
CC       Multi-pass membrane protein {ECO:0000250|UniProtKB:Q8L9J7}.
CC   -!- DEVELOPMENTAL STAGE: Accumulates rapidly but transiently before nodule
CC       emergence and in the first days of nodulation. Later disappears in two-
CC       week-old nodules. {ECO:0000269|PubMed:8634476}.
CC   -!- INDUCTION: During nodulation. {ECO:0000269|PubMed:8634476}.
CC   -!- SIMILARITY: Belongs to the SWEET sugar transporter family.
CC       {ECO:0000305}.
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DR   EMBL; Y08726; CAA69976.1; -; mRNA.
DR   TCDB; 2.A.123.1.1; the sweet, pq-loop, saliva, mtn3 (sweet) family.
DR   ExpressionAtlas; P93332; differential.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR   GO; GO:0051119; F:sugar transmembrane transporter activity; ISS:UniProtKB.
DR   InterPro; IPR004316; SWEET_sugar_transpr.
DR   Pfam; PF03083; MtN3_slv; 2.
PE   2: Evidence at transcript level;
KW   Cell membrane; Coiled coil; Membrane; Repeat; Sugar transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..268
FT                   /note="Bidirectional sugar transporter N3"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5000146960"
FT   TOPO_DOM        1..7
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        8..28
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        29..42
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        64..70
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..91
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        92..103
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        104..124
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        125..131
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..152
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        153..165
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        166..186
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        187..190
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        191..211
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        212..268
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          10..96
FT                   /note="MtN3/slv 1"
FT   DOMAIN          131..214
FT                   /note="MtN3/slv 2"
FT   REGION          242..268
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          234..262
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   268 AA;  30084 MW;  3EED39B2FAEF148E CRC64;
     MAISHNTLAF TFGMLGNVIS FLVFLAPIST FYRIYKKKST EGFQSLPYLV ALFSSMLWLY
     YALLKKDAFL LITINSFGCV VETIYIILYI IYAPRDARNL TFKLLSAMNV GSFALILIVT
     NYAVHGPLRV QVLGWVCVSL SVSVFAAPLS IVAQVVRTKS VEFMPFNLSF TLTLSATMWF
     GYGFFLKDIC IXLPNVLGXV LGLLQMLLYA IYRNGGEKAM KKEKKAPIEP PKSIVIETQL
     EKIEQEKKNK DDDNEEKDKS EEPIGCGV