ID   NOD3_PEA                Reviewed;         334 AA.
AC   E9KID3;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   05-APR-2011, sequence version 1.
DT   03-AUG-2022, entry version 16.
DE   RecName: Full=Hydroxyproline O-arabinosyltransferase NOD3 {ECO:0000305};
DE            EC=2.4.2.58 {ECO:0000305};
DE   AltName: Full=Protein NODULATION 3 {ECO:0000305};
DE            Short=PsNOD3 {ECO:0000303|PubMed:21802605};
DE   Flags: Fragment;
GN   Name=NOD3 {ECO:0000303|PubMed:21742814};
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=21742814; DOI=10.1104/pp.111.178756;
RA   Schnabel E.L., Kassaw T.K., Smith L.S., Marsh J.F., Oldroyd G.E.,
RA   Long S.R., Frugoli J.A.;
RT   "The ROOT DETERMINED NODULATION1 gene regulates nodule number in roots of
RT   Medicago truncatula and defines a highly conserved, uncharacterized plant
RT   gene family.";
RL   Plant Physiol. 157:328-340(2011).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=21802605; DOI=10.1016/j.plantsci.2010.07.007;
RA   Novak K.;
RT   "Early action of pea symbiotic gene NOD3 is confirmed by adventitious root
RT   phenotype.";
RL   Plant Sci. 179:472-478(2010).
CC   -!- FUNCTION: Probable glycosyltransferase involved in the O-
CC       arabinosylation of several proteins including extensins and small
CC       signaling peptides (By similarity). Catalyzes the transfer of the
CC       initial L-arabinose to the hydroxyl group of Hyp residues (By
CC       similarity). Probably involved in the arabinosylation of CLAVATA3/ESR-
CC       related (CLE) signaling peptides that move from root to shoot, to
CC       interact with receptor kinase signaling that regulates nodulation (By
CC       similarity). Involved in long distance nodulation signaling events
CC       (PubMed:21742814, PubMed:21802605). Involved in the autoregulation of
CC       nodulation (AON), a long distance systemic signaling from root to shoot
CC       and back again, which allows legumes to limit the number of root
CC       nodules formed based on available nitrogen and previous rhizobial
CC       colonization (PubMed:21742814, PubMed:21802605).
CC       {ECO:0000250|UniProtKB:E9KID2, ECO:0000269|PubMed:21742814,
CC       ECO:0000269|PubMed:21802605}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=trans-4-hydroxy-L-prolyl-[protein] + UDP-beta-L-
CC         arabinofuranose = H(+) + O-(beta-L-arabinofuranosyl)-trans-4-hydroxy-
CC         L-prolyl-[protein] + UDP; Xref=Rhea:RHEA:49472, Rhea:RHEA-COMP:12408,
CC         Rhea:RHEA-COMP:12409, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:61463, ChEBI:CHEBI:61965, ChEBI:CHEBI:131610;
CC         EC=2.4.2.58; Evidence={ECO:0000305};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:49473;
CC         Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000250|UniProtKB:E9KID2}; Single-pass type II membrane protein
CC       {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Dramatic increase in root nodule number when
CC       inoculated with Rhizobium strains (PubMed:21742814, PubMed:21802605).
CC       Inhibition of root growth in both the presence and absence of rhizobia
CC       (PubMed:21742814, PubMed:21802605). {ECO:0000269|PubMed:21742814,
CC       ECO:0000269|PubMed:21802605}.
CC   -!- SIMILARITY: Belongs to the RDN family. {ECO:0000305}.
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DR   EMBL; GU580938; ADV35717.1; -; Genomic_DNA.
DR   EMBL; GU580939; ADV35718.1; -; mRNA.
DR   AlphaFoldDB; E9KID3; -.
DR   EnsemblPlants; Psat0s3806g0040.1; Psat0s3806g0040.1.cds; Psat0s3806g0040.
DR   Gramene; Psat0s3806g0040.1; Psat0s3806g0040.1.cds; Psat0s3806g0040.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0102562; F:hydroxyproline O-arbinofuranose transferase activity; IEA:RHEA.
DR   InterPro; IPR044845; HPAT/SRGT1-like.
DR   PANTHER; PTHR31485; PTHR31485; 1.
PE   2: Evidence at transcript level;
KW   Glycosyltransferase; Golgi apparatus; Membrane; Signal-anchor; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           <1..>334
FT                   /note="Hydroxyproline O-arabinosyltransferase NOD3"
FT                   /id="PRO_5007654557"
FT   TRANSMEM        <1..18
FT                   /note="Helical; Signal-anchor"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
FT   NON_TER         334
SQ   SEQUENCE   334 AA;  38110 MW;  378F01FCF6DB312A CRC64;
     LLMVLGFFFA TYNLVSMIVG HKVGSDLGSI VDGKVEFTNT KSKFHVAVTA TDAAYSQWQC
     RIMYYWYKKA KDMPGSAMGK FTRILHSGKE DQLMNEIPTF VVDPLPDGLD RGYIVLNRPW
     AFVQWLEKAV IDEEYILMAE PDHIFVNPLP NLASENEPAG YPFFYIKPAE NEKIMRKFYP
     KEKGPVTDVD PIGNSPVIIH KYLLEEIAPT WVNVSLRMKD DPETDKVFGW VLEMYAYAVA
     SALHGIKHTL RKDFMLQPPW DLEVGKTFII HYTYGCDYNL KGKLTYGKIG EWRFDKRSYL
     MSPPPKNISL PPPGVPESVV RLVKMVNEAT ANIP