NOD4A_XENLA
ID NOD4A_XENLA Reviewed; 402 AA.
AC O13048;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Nodal homolog 4-A;
DE AltName: Full=Nodal-related protein 4-A;
DE AltName: Full=Xnr-4;
DE AltName: Full=Xnr4;
DE Flags: Precursor;
GN Name=nodal4-a; Synonyms=nr4 {ECO:0000312|EMBL:AAC60127.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAC60127.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC TISSUE=Gastrula {ECO:0000269|PubMed:9133442};
RX PubMed=9133442; DOI=10.1006/dbio.1997.8510;
RA Joseph E.M., Melton D.A.;
RT "Xnr4: a Xenopus nodal-related gene expressed in the Spemann organizer.";
RL Dev. Biol. 184:367-372(1997).
RN [2] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=10375512; DOI=10.1242/dev.126.14.3229;
RA Osada S., Wright C.V.E.;
RT "Xenopus nodal-related signaling is essential for mesendodermal patterning
RT during early embryogenesis.";
RL Development 126:3229-3240(1999).
RN [3] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=10572051; DOI=10.1242/dev.126.24.5759;
RA Kofron M., Demel T., Xanthos J., Lohr J., Sun B., Sive H., Osada S.,
RA Wright C.V.E., Wylie C., Heasman J.;
RT "Mesoderm induction in Xenopus is a zygotic event regulated by maternal
RT VegT via TGFbeta growth factors.";
RL Development 126:5759-5770(1999).
RN [4] {ECO:0000305}
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=10683171; DOI=10.1242/dev.127.6.1173;
RA Agius E., Oelgeschlaeger M., Wessely O., Kemp C., De Robertis E.M.;
RT "Endodermal Nodal-related signals and mesoderm induction in Xenopus.";
RL Development 127:1173-1183(2000).
RN [5] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=11784097; DOI=10.1006/dbio.2001.0493;
RA Onuma Y., Takahashi S., Yokota C., Asashima M.;
RT "Multiple nodal-related genes act coordinately in Xenopus embryogenesis.";
RL Dev. Biol. 241:94-105(2002).
RN [6] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=16651540; DOI=10.1242/dev.02358;
RA Sinner D., Kirilenko P., Rankin S., Wei E., Howard L., Kofron M.,
RA Heasman J., Woodland H.R., Zorn A.M.;
RT "Global analysis of the transcriptional network controlling Xenopus
RT endoderm formation.";
RL Development 133:1955-1966(2006).
CC -!- FUNCTION: Cooperation and regulatory loops of multiple nodals are
CC essential for mesendoderm patterning in early embryos. Plays a role in
CC mesoderm formation and may be required for neural development.
CC {ECO:0000269|PubMed:10375512, ECO:0000269|PubMed:10572051,
CC ECO:0000269|PubMed:11784097, ECO:0000269|PubMed:16651540,
CC ECO:0000269|PubMed:9133442}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000250|UniProtKB:P43021}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q91620}.
CC -!- TISSUE SPECIFICITY: During blastula stages, expressed in the endoderm
CC at a higher level dorsally than ventrally. Expressed in the deep cells
CC of the Spemann organizer at the gastrula stage. Expressed in the
CC notochord (a derivative of the organizer) and neural tube during the
CC neural stages. {ECO:0000269|PubMed:10683171,
CC ECO:0000269|PubMed:9133442}.
CC -!- DEVELOPMENTAL STAGE: Expressed zygotically. First expressed at the
CC midblastula transition (MBT). {ECO:0000269|PubMed:10683171,
CC ECO:0000269|PubMed:9133442}.
CC -!- INDUCTION: By dorsal-mesoderm inducing signals including vegt and other
CC nodal-related proteins. By sox17. {ECO:0000269|PubMed:10375512,
CC ECO:0000269|PubMed:10572051, ECO:0000269|PubMed:10683171,
CC ECO:0000269|PubMed:11784097, ECO:0000269|PubMed:16651540}.
CC -!- SIMILARITY: Belongs to the TGF-beta family. {ECO:0000255}.
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DR EMBL; U79162; AAC60127.1; -; mRNA.
DR RefSeq; NP_001081816.1; NM_001088347.1.
DR AlphaFoldDB; O13048; -.
DR GeneID; 398068; -.
DR KEGG; xla:398068; -.
DR CTD; 398068; -.
DR Xenbase; XB-GENE-864954; nodal.L.
DR OrthoDB; 1518399at2759; -.
DR Proteomes; UP000186698; Chromosome 7L.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR001839; TGF-b_C.
DR InterPro; IPR015615; TGF-beta-rel.
DR InterPro; IPR017948; TGFb_CS.
DR PANTHER; PTHR11848; PTHR11848; 1.
DR Pfam; PF00019; TGF_beta; 1.
DR SMART; SM00204; TGFB; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
DR PROSITE; PS00250; TGF_BETA_1; 1.
DR PROSITE; PS51362; TGF_BETA_2; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Developmental protein; Disulfide bond;
KW Glycoprotein; Growth factor; Reference proteome; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT PROPEP 19..278
FT /evidence="ECO:0000255"
FT /id="PRO_0000334505"
FT CHAIN 279..402
FT /note="Nodal homolog 4-A"
FT /evidence="ECO:0000255"
FT /id="PRO_0000334506"
FT CARBOHYD 37
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 238
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 340
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 302..368
FT /evidence="ECO:0000250|UniProtKB:P43021"
FT DISULFID 331..399
FT /evidence="ECO:0000250|UniProtKB:P43021"
FT DISULFID 335..401
FT /evidence="ECO:0000250|UniProtKB:P43021"
FT DISULFID 367
FT /note="Interchain"
FT /evidence="ECO:0000250|UniProtKB:P43021"
SQ SEQUENCE 402 AA; 46271 MW; 928B46B550443288 CRC64;
MHLYFYCLIL LFVPGGNSLG INSYLKHMSN KPQDHVNRTK TVDSKDLAAL PLSSYMLNLY
QSFHHSELNH GTEGAPSLPS NHRADIIRSL AAKSFDHGGS RWTLVFDFSS LSQEEEHQLA
EVRFDFRAFE GAISAEMEVM VDFLHQSSSC QSISGWCQSY LYVGSLTGTL RSRSSDTWVT
FEATDIIHKW FERNEKGKSR YEDREKQLKK LPRAKSAERR YQQQNTEDQQ IVMMVYSNIS
KKERLSGTAT LLQDAAHSKY LVVMPGIQTI AHTRRHRRSH IFKEHVMGMK HVPPADSSRT
LCRRVDFFVD FKQIGWDSWI IHPMKYNAYR CEGECPSPVN ESVKPNNHAY MQSLLNYYVK
GKAPEVCCVP IRMSSLSMVY YDHDDIAFQN HEGMIVEECG CQ
