NODB_RHILV
ID NODB_RHILV Reviewed; 216 AA.
AC P04339;
DT 20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-1987, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Chitooligosaccharide deacetylase;
DE EC=3.5.1.-;
DE AltName: Full=Nodulation protein B;
GN Name=nodB;
OS Rhizobium leguminosarum bv. viciae.
OG Plasmid sym pRL1JI.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=387;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=248;
RX PubMed=6514582; DOI=10.1093/nar/12.24.9497;
RA Rossen L., Johnston A.W.B., Downie J.A.;
RT "DNA sequence of the Rhizobium leguminosarum nodulation genes nodAB and C
RT required for root hair curling.";
RL Nucleic Acids Res. 12:9497-9508(1984).
CC -!- FUNCTION: Is involved in generating a small heat-stable compound (Nod),
CC an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in
CC various plant protoplasts.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the polysaccharide deacetylase family.
CC {ECO:0000305}.
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DR EMBL; Y00548; CAA68620.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X01650; CAA25813.1; -; Genomic_DNA.
DR PIR; A03485; ZZZRBL.
DR AlphaFoldDB; P04339; -.
DR SMR; P04339; -.
DR OMA; NHAMRDE; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016810; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR002509; NODB_dom.
DR InterPro; IPR026402; Nodulat_NodB.
DR Pfam; PF01522; Polysacc_deac_1; 1.
DR SUPFAM; SSF88713; SSF88713; 1.
DR TIGRFAMs; TIGR04243; nodulat_NodB; 1.
DR PROSITE; PS51677; NODB; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Hydrolase; Metal-binding; Nodulation; Plasmid.
FT CHAIN 1..216
FT /note="Chitooligosaccharide deacetylase"
FT /id="PRO_0000172754"
FT DOMAIN 21..213
FT /note="NodB homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01014"
FT ACT_SITE 28
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 174
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 79
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 83
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT SITE 148
FT /note="Raises pKa of active site His"
FT /evidence="ECO:0000250"
SQ SEQUENCE 216 AA; 23632 MW; 65B751C65F8ECF58 CRC64;
MKRPAYMSEV PVNHTSGQEA RCVYLTFDDG PNPFCTPQIL DVLAEHRVPA TFFAIGSYVK
DHPELIRRLV AEGHDVANHT MTHPDLATCD PKDVKREIDE AHQAIVSACP QALVRHLRAP
YGVWTEDVLS ASVRAGLGAV HWSADPRDWS CPGVDVIVDE VLAAARPGAI VLLHDGCPPD
EVEQCSLAGL RDQTLIALSR IIPALHSRGF EIRSLP