ID   NODE_RHIS3              Reviewed;         402 AA.
AC   P72331;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Nodulation protein E;
DE   AltName: Full=Host-specificity of nodulation protein B;
DE            EC=2.3.1.-;
GN   Name=nodE;
OS   Rhizobium sp. (strain N33).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=103798;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9100384; DOI=10.1094/mpmi.1997.10.3.401;
RA   Cloutier J., Laberge S., Antoun H.;
RT   "Sequence and mutational analysis of the 6.7-kb region containing nodAFEG
RT   genes of Rhizobium sp. strain N33: evidence of DNA rearrangements.";
RL   Mol. Plant Microbe Interact. 10:401-406(1997).
CC   -!- FUNCTION: Proposed to synthesize NOD factor fatty acyl chain. Involved
CC       in the synthesis of a highly unsaturated fatty acid moiety, which forms
CC       part of a lipo-oligosaccharide that is responsible for host
CC       specificity.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP
CC       synthases family. {ECO:0000305}.
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DR   EMBL; U53327; AAB16894.1; -; Genomic_DNA.
DR   AlphaFoldDB; P72331; -.
DR   SMR; P72331; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR   CDD; cd00834; KAS_I_II; 1.
DR   Gene3D; 3.40.47.10; -; 1.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR020615; Thiolase_acyl_enz_int_AS.
DR   PANTHER; PTHR11712; PTHR11712; 1.
DR   Pfam; PF00109; ketoacyl-synt; 1.
DR   Pfam; PF02801; Ketoacyl-synt_C; 1.
DR   SMART; SM00825; PKS_KS; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Nodulation; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..402
FT                   /note="Nodulation protein E"
FT                   /id="PRO_0000180355"
FT   TRANSMEM        329..348
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        162
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
SQ   SEQUENCE   402 AA;  42014 MW;  05293282A1097AFE CRC64;
     MDRRVVITGI GGLCGLGTDA PSIWKEMREG RSAIGPIVTS ELHGLTGTIG AEIKTLPEHD
     IDRKQLVTMD RFSLLAVLAA REAMRQAGLS CDERNAYRFG AIVGVGGSGW EAIEASYRAL
     LLNGARRAGV MDVPKAMPSA AAGQVSMSLG LRGPVFGVTS ACASANHAIA SAVDQIRCGR
     ADVMLAGGSD APFVFCVVKA WEAMRVIAPD TCRPFSSDRR GLVLGEGAGM AVLESYEHAT
     ARGATIIAEI AGIGLSADAF NLVSPAVEGP EAAMRACLAD AGLNVQDVDY INAHGTGTKA
     NDRMETEAIK RVFGGHANSM SISSTKSMHA HCLGAASALE MIACVMAIQE GVVPPTANYR
     EPDPDCDLDV TPNVARERKV RVALSNAFAM AGMNAVLAFR QV