ID   NODO_RHILV              Reviewed;         284 AA.
AC   P15728;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Nodulation protein O;
GN   Name=nodO; Synonyms=nolR;
OS   Rhizobium leguminosarum bv. viciae.
OG   Plasmid sym pRL1JI.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=387;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-8.
RC   STRAIN=8401;
RX   PubMed=2303029; DOI=10.1002/j.1460-2075.1990.tb08117.x;
RA   Economou A., Hamilton W.D.O., Johnston A.W.B., Downie J.A.;
RT   "The Rhizobium nodulation gene nodO encodes a Ca2(+)-binding protein that
RT   is exported without N-terminal cleavage and is homologous to haemolysin and
RT   related proteins.";
RL   EMBO J. 9:349-354(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 4-18.
RX   PubMed=2687250; DOI=10.1128/jb.171.12.6764-6770.1989;
RA   de Maagd R.A., Wijfjes A.H.M., Spaink H.P., Ruiz-Sainz J.E.,
RA   Wijffelman C.A., Okker R.J.H., Lugtenberg B.J.J.;
RT   "nodO, a new nod gene of the Rhizobium leguminosarum biovar viciae sym
RT   plasmid pRL1JI, encodes a secreted protein.";
RL   J. Bacteriol. 171:6764-6770(1989).
CC   -!- FUNCTION: The NodO protein may play a role in nodule development by
CC       direct interaction with the root hair cells or some other plant surface
CC       in a calcium-dependent manner.
CC   -!- SUBCELLULAR LOCATION: Secreted. Note=By a mechanism that does not
CC       involve an N-terminal signal peptide.
CC   -!- INDUCTION: By naringenin (flavonoid).
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DR   EMBL; X17285; CAA35178.1; -; Genomic_DNA.
DR   EMBL; M29532; AAA26341.1; -; Genomic_DNA.
DR   PIR; A43721; A43721.
DR   PIR; S08385; S08385.
DR   RefSeq; WP_011654216.1; NZ_WIFB01000040.1.
DR   AlphaFoldDB; P15728; -.
DR   SMR; P15728; -.
DR   OMA; ANHAHAF; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   Gene3D; 2.150.10.10; -; 2.
DR   InterPro; IPR018511; Hemolysin-typ_Ca-bd_CS.
DR   InterPro; IPR001343; Hemolysn_Ca-bd.
DR   InterPro; IPR011049; Serralysin-like_metalloprot_C.
DR   Pfam; PF00353; HemolysinCabind; 3.
DR   SUPFAM; SSF51120; SSF51120; 1.
DR   PROSITE; PS00330; HEMOLYSIN_CALCIUM; 1.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Metal-binding; Nodulation; Plasmid;
KW   Repeat; Secreted.
FT   CHAIN           1..284
FT                   /note="Nodulation protein O"
FT                   /id="PRO_0000096911"
FT   REPEAT          13..30
FT                   /note="Hemolysin-type calcium-binding 1"
FT   REPEAT          31..48
FT                   /note="Hemolysin-type calcium-binding 2"
FT   REPEAT          58..75
FT                   /note="Hemolysin-type calcium-binding 3"
FT   REPEAT          94..111
FT                   /note="Hemolysin-type calcium-binding 4"
FT   REPEAT          112..129
FT                   /note="Hemolysin-type calcium-binding 5"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          208..222
FT                   /note="Export signal (aspartic acid box)"
FT                   /evidence="ECO:0000255"
FT   BINDING         100
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000305|PubMed:2303029"
FT   BINDING         109
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000305|PubMed:2303029"
FT   BINDING         118
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000305|PubMed:2303029"
FT   BINDING         127
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000305|PubMed:2303029"
SQ   SEQUENCE   284 AA;  30002 MW;  9CA41DCFCBDF8E15 CRC64;
     MNIKGSDNGS FIKGSPENDI IDGGKKNDWI DAGNGDDRIK AGDGQDSITA GPGHDIVWAG
     KGSDVIHADG GDDLLYSDAS YPLYVTDPHR VIPHSGEGDD VLYAGPGSDI LVAGDGADVL
     TGGDDGDAFV FRFHDPMVGT THCYTSVMDF DTKQDRFVLD AADFGGDRNL FDANFINHSK
     GFPGEFVDTF YNGAAEGAHG EHVVVITDRG FASAAAAATA IDHEARGDII VFHDQKTLGQ
     DGETHGATLA YVDSANHAHA FAHVDNLHDM SDLTSLTAEN FGFI