ID   NODP_RHISB              Reviewed;         299 AA.
AC   O07308;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Sulfate adenylyltransferase subunit 2;
DE            EC=2.7.7.4;
DE   AltName: Full=ATP-sulfurylase small subunit;
DE   AltName: Full=Nodulation protein P;
DE   AltName: Full=Sulfate adenylate transferase;
DE            Short=SAT;
GN   Name=nodP;
OS   Rhizobium sp. (strain BR816).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Ensifer; unclassified Ensifer.
OX   NCBI_TaxID=1057002;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9421916; DOI=10.1099/00221287-143-12-3933;
RA   Laeremans T., Coolsaet N., Verreth C., Snoeck C., Hellings N.,
RA   Vanderleyden J., Martinez-Romero E.;
RT   "Functional redundancy of genes for sulphate activation enzymes in
RT   Rhizobium sp. BR816.";
RL   Microbiology 143:3933-3942(1997).
CC   -!- FUNCTION: Proposed to provide activated sulfate for transfer to nod
CC       factor.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H(+) + sulfate = adenosine 5'-phosphosulfate +
CC         diphosphate; Xref=Rhea:RHEA:18133, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16189, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58243; EC=2.7.7.4;
CC   -!- SUBUNIT: Sulfate-activating enzymes, NodP and NodQ, may be physically
CC       associated. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the PAPS reductase family. CysD subfamily.
CC       {ECO:0000305}.
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DR   EMBL; U59507; AAB95248.1; -; Genomic_DNA.
DR   RefSeq; WP_026187124.1; NZ_KB905370.1.
DR   AlphaFoldDB; O07308; -.
DR   SMR; O07308; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004781; F:sulfate adenylyltransferase (ATP) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:UniProtKB-UniRule.
DR   GO; GO:0019419; P:sulfate reduction; IEA:InterPro.
DR   CDD; cd01713; PAPS_reductase; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00064; Sulf_adenylyltr_sub2; 1.
DR   InterPro; IPR002500; PAPS_reduct.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR011784; SO4_adenylTrfase_ssu.
DR   Pfam; PF01507; PAPS_reduct; 1.
DR   PIRSF; PIRSF002936; CysDAde_trans; 1.
DR   TIGRFAMs; TIGR02039; CysD; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Nodulation; Nucleotide-binding; Nucleotidyltransferase;
KW   Transferase.
FT   CHAIN           1..299
FT                   /note="Sulfate adenylyltransferase subunit 2"
FT                   /id="PRO_0000100687"
SQ   SEQUENCE   299 AA;  34494 MW;  4F57C5F1983168F2 CRC64;
     MSLANLRRLE AEAIHVLREV VATFSNPVVL YSIGKDSSVL LHLAMKAFFP AKPPFPFLHV
     DTTWKFREMI AFRDRMAREL GFNLLVHTNQ DGVDQGIGPF THGSNLHTHV MKTMALRQAL
     ETYGFDAALA GARRDEEKSR AKERMFSIRN AQHGWDPKRQ RPEMWKTYNT RVGAGETMRV
     FPLSNWTEFD IWQYILQENI PIVPLYFAAR RPVVRRDGML IMVDDERMPI EPGEEVMDRM
     VRFRTLGCYP LTGAIDSEAA TVPDILREML TVRTSERLSR LIDTDEAGAM EKKKREGYF