ID   NOE4_RHIML              Reviewed;         401 AA.
AC   P06231;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Nodulation protein E;
DE   AltName: Full=Host-specificity of nodulation protein B;
DE            EC=2.3.1.-;
GN   Name=nodE; Synonyms=hsnB;
OS   Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti).
OG   Plasmid sym pRme41b.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=382;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3731273; DOI=10.1016/0092-8674(86)90654-9;
RA   Horvath B., Kondorosi E., John M., Schmidt J., Toeroek I., Gyoergypal Z.,
RA   Barabas I., Wieneke U., Schell J., Kondorosi A.;
RT   "Organization, structure and symbiotic function of Rhizobium meliloti
RT   nodulation genes determining host specificity for alfalfa.";
RL   Cell 46:335-343(1986).
CC   -!- FUNCTION: Proposed to synthesize NOD factor fatty acyl chain. Involved
CC       in the synthesis of a highly unsaturated fatty acid moiety, which forms
CC       part of a lipo-oligosaccharide that is responsible for host
CC       specificity.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP
CC       synthases family. {ECO:0000305}.
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DR   EMBL; M14052; AAA26289.1; -; Genomic_DNA.
DR   AlphaFoldDB; P06231; -.
DR   SMR; P06231; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR   CDD; cd00834; KAS_I_II; 1.
DR   Gene3D; 3.40.47.10; -; 1.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR020615; Thiolase_acyl_enz_int_AS.
DR   PANTHER; PTHR11712; PTHR11712; 1.
DR   Pfam; PF00109; ketoacyl-synt; 1.
DR   Pfam; PF02801; Ketoacyl-synt_C; 1.
DR   SMART; SM00825; PKS_KS; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Nodulation; Plasmid;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..401
FT                   /note="Nodulation protein E"
FT                   /id="PRO_0000180354"
FT   TRANSMEM        328..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        161
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
SQ   SEQUENCE   401 AA;  41667 MW;  C1EC17B190077CB1 CRC64;
     MDRRVVITGM GGLCGLGTDT TSIWKWSARR RSAIGPVLNT ELHGLKGIVG AEIKALPDHN
     IDRKQLVSMD RISVLAVIAA HEAMRQAGLS CNEGNALRFG ATVGVGLGGW DATEKAYRPS
     LSTGGRTEIF TGVKAMPSAA ACQVSMSLGL RGPVFGVTSA CSSANHAIAS AVDQIKCGRA
     DVMLAGGSDA PLVWIVLKAW EAMRALAPDT CRPFSAGRKG VVLGEGAGMA VLESYEHATA
     RGATILAEVA GVGLSADAFH ITAPAVHGPE SAMRACLADA GLNAEDVDYL NAHGTGTKAN
     DQNETTAIKR VFGDHAYSMS ISSTKSTHAH CIGAASALEM IACVMAIQEG VVPPTANYRE
     PDPDCDLDVT PNVPRERKVR VAMSNAFAMG GTNAVLAFKQ V