NOEI_SINFN
ID NOEI_SINFN Reviewed; 243 AA.
AC P55473;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=2-O-methyltransferase NoeI;
DE EC=2.1.1.-;
DE AltName: Full=Nodulation protein NoeI;
GN Name=noeI; OrderedLocusNames=NGR_a03470; ORFNames=y4hC;
OS Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG Plasmid sym pNGR234a.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=394;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=9163424; DOI=10.1038/387394a0;
RA Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA Perret X.;
RT "Molecular basis of symbiosis between Rhizobium and legumes.";
RL Nature 387:394-401(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=19376903; DOI=10.1128/aem.00515-09;
RA Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT systems.";
RL Appl. Environ. Microbiol. 75:4035-4045(2009).
RN [3]
RP FUNCTION IN METHYLATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=9575146; DOI=10.1074/jbc.273.20.12047;
RA Jabbouri S., Relic B., Hanin M., Kamalaprija P., Burger U., Prome D.,
RA Prome J.C., Broughton W.J.;
RT "nolO and noeI (HsnIII) of Rhizobium sp. NGR234 are involved in 3-O-
RT carbamoylation and 2-O-methylation of Nod factors.";
RL J. Biol. Chem. 273:12047-12055(1998).
CC -!- FUNCTION: Required for 2-O-methylation of the fucosyl group of Nod
CC factors. {ECO:0000269|PubMed:9575146}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Disruption of the gene has no effect on
CC nodulation. {ECO:0000269|PubMed:9575146}.
CC -!- SIMILARITY: Belongs to the FkbM methyltransferase family.
CC {ECO:0000305}.
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DR EMBL; U00090; AAB91691.1; -; Genomic_DNA.
DR RefSeq; NP_443879.1; NC_000914.2.
DR RefSeq; WP_010875361.1; NC_000914.2.
DR AlphaFoldDB; P55473; -.
DR SMR; P55473; -.
DR STRING; 394.NGR_a03470; -.
DR EnsemblBacteria; AAB91691; AAB91691; NGR_a03470.
DR KEGG; rhi:NGR_a03470; -.
DR eggNOG; COG0275; Bacteria.
DR HOGENOM; CLU_068034_3_2_5; -.
DR OMA; WMDVQGA; -.
DR OrthoDB; 1340956at2; -.
DR Proteomes; UP000001054; Plasmid sym pNGR234a.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR006342; FkbM_mtfrase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF05050; Methyltransf_21; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR01444; fkbM_fam; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Methyltransferase; Nodulation; Plasmid; Reference proteome;
KW Transferase.
FT CHAIN 1..243
FT /note="2-O-methyltransferase NoeI"
FT /id="PRO_0000096924"
SQ SEQUENCE 243 AA; 27735 MW; D22A60D7AAAB87C1 CRC64;
MEVGRYLKEG LLISLQQRLR EFAEGIRKPT SSLKVHRQIV SLLEKPDPVI LDIGCNDGSD
ARRFLQLRPK AQLFCFEPDP RAAARCRENM GPLDRMRLFE VAISDRNGRI DFHPSNGDGD
AKEWDLSGSI RQPKNHLSEY QWVRFDGPIS VETRRLDDWC SEAGLESIDL IWMDVQGAES
DVIAGGKETL TKTRFIYTEY SDQELYEGQL PLRAILDLLP SFELVAQFPR GVEGDVLLRN
TKL
