NOF1_ARATH
ID NOF1_ARATH Reviewed; 754 AA.
AC Q8H1E7; Q93ZS0; Q9FZ64;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Protein NUCLEOLAR FACTOR 1 {ECO:0000303|PubMed:20877469};
GN Name=NOF1 {ECO:0000303|PubMed:20877469};
GN OrderedLocusNames=At1g17690 {ECO:0000312|EMBL:AEE29622.1};
GN ORFNames=F11A6.3 {ECO:0000312|EMBL:AAF99813.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Wassilewskija;
RX PubMed=20877469; DOI=10.1371/journal.pone.0012829;
RA Harscoet E., Dubreucq B., Palauqui J.-C., Lepiniec L.;
RT "NOF1 encodes an Arabidopsis protein involved in the control of rRNA
RT expression.";
RL PLoS ONE 5:e12829-e12829(2010).
RN [5]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH THAL.
RC STRAIN=cv. Columbia;
RX PubMed=27792779; DOI=10.1371/journal.pgen.1006408;
RA Chen Y.-J.C., Wang H.-J., Jauh G.-Y.;
RT "Dual role of a SAS10/C1D family protein in ribosomal RNA gene expression
RT and processing is essential for reproduction in Arabidopsis thaliana.";
RL PLoS Genet. 12:e1006408-e1006408(2016).
CC -!- FUNCTION: DEAD-box RNA helicase-like protein required for pre-18S rRNA
CC processing, specifically at sites A0, A1, and A2 (By similarity).
CC Involved in the control of rRNA expression (PubMed:20877469). Required
CC for embryo development and female gametogenesis (PubMed:20877469).
CC {ECO:0000250|UniProtKB:P40498, ECO:0000269|PubMed:20877469}.
CC -!- SUBUNIT: Component of the ribosomal small subunit (SSU) processome
CC composed of at least 40 protein subunits and snoRNA U3 (By similarity).
CC Interacts with THAL in the nucleus (PubMed:27792779).
CC {ECO:0000250|UniProtKB:P40498, ECO:0000269|PubMed:27792779}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:20877469,
CC ECO:0000269|PubMed:27792779}.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in differentiating cells
CC in young tissues such as floral buds, ovules, embryos, secondary roots,
CC pollen, young seedlings and vascular bundles (PubMed:20877469).
CC Observed ubiquitously (PubMed:20877469). {ECO:0000269|PubMed:20877469}.
CC -!- DISRUPTION PHENOTYPE: Abnormal female gametogenesis and altered embryo
CC development with a disturbed cellular division pattern
CC (PubMed:20877469). Increased rRNA expression and hypomethylation of
CC rRNA promoters (PubMed:20877469). {ECO:0000269|PubMed:20877469}.
CC -!- SIMILARITY: Belongs to the UTP25 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF99813.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC034257; AAF99813.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29622.1; -; Genomic_DNA.
DR EMBL; AY056300; AAL07149.1; -; mRNA.
DR EMBL; AY150441; AAN12983.1; -; mRNA.
DR PIR; G86311; G86311.
DR RefSeq; NP_564032.1; NM_101631.2.
DR AlphaFoldDB; Q8H1E7; -.
DR IntAct; Q8H1E7; 1.
DR STRING; 3702.AT1G17690.1; -.
DR PaxDb; Q8H1E7; -.
DR PRIDE; Q8H1E7; -.
DR ProteomicsDB; 193307; -.
DR EnsemblPlants; AT1G17690.1; AT1G17690.1; AT1G17690.
DR GeneID; 838345; -.
DR Gramene; AT1G17690.1; AT1G17690.1; AT1G17690.
DR KEGG; ath:AT1G17690; -.
DR Araport; AT1G17690; -.
DR TAIR; locus:2007933; AT1G17690.
DR eggNOG; KOG2340; Eukaryota.
DR HOGENOM; CLU_018705_2_1_1; -.
DR InParanoid; Q8H1E7; -.
DR OMA; KYFRQTI; -.
DR OrthoDB; 257318at2759; -.
DR PhylomeDB; Q8H1E7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8H1E7; baseline and differential.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0032040; C:small-subunit processome; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IBA:GO_Central.
DR GO; GO:0034511; F:U3 snoRNA binding; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0009303; P:rRNA transcription; IMP:TAIR.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010678; UTP25.
DR PANTHER; PTHR12933; PTHR12933; 1.
DR Pfam; PF06862; UTP25; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ribonucleoprotein; Ribosome biogenesis;
KW rRNA processing.
FT CHAIN 1..754
FT /note="Protein NUCLEOLAR FACTOR 1"
FT /id="PRO_0000454728"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 69..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 390..413
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..28
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..115
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..160
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 732
FT /note="E -> G (in Ref. 3; AAL07149)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 754 AA; 86920 MW; 93B7397E56468E9F CRC64;
MAPNATGVLK RHRSHEKFDK KRDTKKHKHV EKTIVSNPST DSPEESSIEA ESEAMVYREP
TQYQNLLVSL GSSNKVVADM NKRRQREEEG KSDTEEDEDD EDEDEEENSG SDDLSSTDGE
DDKSQGDDQE TLGGLTDDTQ EDNDNQSEEE DPDDYETDEE VHELSTNGQS FVDASSSISA
FSEHLSHKLS SEEVETLPKG KWKFKWESPA FDMPNCKWKG TSENFLDGIQ SDAPYGLKPK
LYNHWLQLYK KCGGKDLDSS KRRKFFSICN SYLDILHSNK KPFYHCGSDE DSSAMDAYLM
HSLNHIFKTR DLVKKNESKI AKHRETSEEE ILSDDGFLDQ GFTRPKVLIL LPLRSIAFRV
VKRLIQLTPE SQRVNVEHLD RFNDEFGCEE DTDDCDGEKT TSKNGNSIKQ KSSKPSDWQA
LFGANNNDDE FMLGIKHTRK SIRLYGDFYS SDIIVASPLK LHMAIGAAEE NKERDVDYLS
SIEVLVIDHA DIISMQNWSF LATVVDYLNR LPTKQHGTNV MRIRPLYLDG HARFYRQSII
LSSYLTPEMN SLFGRHCLNY KGKMKMACEY KGVLEKVLLP VRQIYERFDA ASITQVDDAR
LEYFTKKIFP KIKDSVQGGV MIFIHSYFEF VRLRNFLNTQ NTSFCLLGDY AKNADISRAR
EQFFVGSRKI MLYTERAYFY KRYKIRGIKN LILYSLPERK EFYPEIMNML EEGSHDMMST
ALFSRFDMLQ LERIVGSTSA KRMITSEKNM FAFC
