NOG1_DROME
ID NOG1_DROME Reviewed; 652 AA.
AC Q9V411;
DT 24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Nucleolar GTP-binding protein 1;
DE AltName: Full=Novel nucleolar protein 1 {ECO:0000303|PubMed:24255106};
GN Name=Non1 {ECO:0000312|FlyBase:FBgn0028473};
GN ORFNames=CG8801 {ECO:0000312|FlyBase:FBgn0028473};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24255106; DOI=10.1073/pnas.1320013110;
RA Moutinho-Pereira S., Stuurman N., Afonso O., Hornsveld M., Aguiar P.,
RA Goshima G., Vale R.D., Maiato H.;
RT "Genes involved in centrosome-independent mitotic spindle assembly in
RT Drosophila S2 cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:19808-19813(2013).
CC -!- FUNCTION: Involved in the biogenesis of the 60S ribosomal subunit (By
CC similarity). Required for normal assembly of the mitotic spindle
CC (PubMed:24255106). May be involved in both centrosome-dependent and
CC centrosome-independent spindle assembly programs (PubMed:24255106).
CC {ECO:0000250|UniProtKB:Q02892, ECO:0000269|PubMed:24255106}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:24255106}.
CC Note=Found in the nucleolus during interphase.
CC {ECO:0000269|PubMed:24255106}.
CC -!- SIMILARITY: Belongs to the TRAFAC class OBG-HflX-like GTPase
CC superfamily. OBG GTPase family. NOG subfamily. {ECO:0000255|PROSITE-
CC ProRule:PRU01047}.
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DR EMBL; AE013599; AAF58945.1; -; Genomic_DNA.
DR EMBL; AF181654; AAD55439.1; -; mRNA.
DR RefSeq; NP_001286247.1; NM_001299318.1.
DR RefSeq; NP_610484.1; NM_136640.5.
DR AlphaFoldDB; Q9V411; -.
DR SMR; Q9V411; -.
DR BioGRID; 61797; 34.
DR DIP; DIP-21969N; -.
DR IntAct; Q9V411; 5.
DR STRING; 7227.FBpp0087637; -.
DR PaxDb; Q9V411; -.
DR DNASU; 35963; -.
DR EnsemblMetazoa; FBtr0088554; FBpp0087637; FBgn0028473.
DR EnsemblMetazoa; FBtr0300649; FBpp0289874; FBgn0028473.
DR GeneID; 35963; -.
DR KEGG; dme:Dmel_CG8801; -.
DR UCSC; CG8801-RA; d. melanogaster.
DR CTD; 35963; -.
DR FlyBase; FBgn0028473; Non1.
DR VEuPathDB; VectorBase:FBgn0028473; -.
DR eggNOG; KOG1490; Eukaryota.
DR GeneTree; ENSGT00390000018475; -.
DR HOGENOM; CLU_011784_4_1_1; -.
DR InParanoid; Q9V411; -.
DR OMA; EWKNDVM; -.
DR OrthoDB; 678655at2759; -.
DR PhylomeDB; Q9V411; -.
DR BioGRID-ORCS; 35963; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Non1; fly.
DR GenomeRNAi; 35963; -.
DR PRO; PR:Q9V411; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0028473; Expressed in oviduct (Drosophila) and 26 other tissues.
DR ExpressionAtlas; Q9V411; baseline and differential.
DR Genevisible; Q9V411; DM.
DR GO; GO:0005730; C:nucleolus; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; HDA:FlyBase.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; ISS:FlyBase.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031167; G_OBG.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR024926; NOG1.
DR InterPro; IPR041623; NOG1_N.
DR InterPro; IPR010674; NOG1_Rossman_fold_dom.
DR InterPro; IPR012973; NOG_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR Pfam; PF06858; NOG1; 1.
DR Pfam; PF17835; NOG1_N; 1.
DR Pfam; PF08155; NOGCT; 1.
DR PIRSF; PIRSF038919; NOG1; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51710; G_OBG; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; GTP-binding; Mitosis; Nucleotide-binding;
KW Nucleus; Reference proteome; Ribosome biogenesis.
FT CHAIN 1..652
FT /note="Nucleolar GTP-binding protein 1"
FT /id="PRO_0000195027"
FT DOMAIN 169..341
FT /note="OBG-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT REGION 501..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 175..182
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT BINDING 221..225
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT BINDING 289..292
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
SQ SEQUENCE 652 AA; 75351 MW; 2F45CC81526D97C8 CRC64;
MSLYNFKKIM VVPPAKDFID IMLSKTQRKT PTVVHKGYKI SRIRAFYTRK VKYTQQNFHD
RLSQIIQDFP KLDDVHPFYA DLMNVLYDKD HYKLALGQLN TARHLVDNVA KDYVRLLKYG
DSLYRCKQLK KAALGRMATI MKRQASNLTY LEQVRQHLSR LPTIDPYSRT IIICGFPNVG
KSSFINKITR ADVEVQPYAF TTKSLYVGHT DYKYLRWQVI DTPGILDHPL EERNVIEMQA
ITALAHLRAC VLYFMDISEQ CGHSLEEQVK LFESIKPLFT NKPLILAINK IDILTPEDLP
EERRAIITKL QEDKNIPVML MSTVQETGVM EVKTEACERL LSYRVDQKMR TKKVDNILNR
LHVAMPAPRD DKLRAPCIPE KASARLLQNA DKAERKRKLE KEIEEEMGDD YTLDLKKNYS
EIPEEERYDI IPEFWQGHNI ADYIDADIFD KLEELEREEG LREESGVYKV PDMTMDETLK
EIREIAKQIR GKRFELRDEK RLSSRKNKPV IPRNKQPKVR DRSVQKLVST MEGLGVDMSG
SENANFTKSV VDLRRGQVAV GSKKVPMQPL LDKESSAVVR KTGQPLKRAP SRDTLGIKNL
AIRKKAQIMA KRDIAKKVTS RGLKGEADRF IGTKMPKHLF SGKRGNGKTD RR
