ID   NOG2_CRYNH              Reviewed;         720 AA.
AC   J9VQ03; Q8J0Y9; Q8J109;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2013, sequence version 2.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Nucleolar GTP-binding protein 2;
GN   Name=NOG2; ORFNames=CNAG_07413;
OS   Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS   CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=235443;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX   PubMed=12455690; DOI=10.1128/ec.1.5.704-718.2002;
RA   Lengeler K.B., Fox D.S., Fraser J.A., Allen A., Forrester K.,
RA   Dietrich F.S., Heitman J.;
RT   "Mating-type locus of Cryptococcus neoformans: a step in the evolution of
RT   sex chromosomes.";
RL   Eukaryot. Cell 1:704-718(2002).
RN   [2]
RP   SEQUENCE REVISION.
RA   Dietrich F.S.;
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX   PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA   Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA   Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA   Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA   Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA   Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA   Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA   Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA   Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA   Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA   Cuomo C.A., Dietrich F.S.;
RT   "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT   grubii reveals complex RNA expression and microevolution leading to
RT   virulence attenuation.";
RL   PLoS Genet. 10:E1004261-E1004261(2014).
CC   -!- FUNCTION: GTPase that associates with pre-60S ribosomal subunits in the
CC       nucleolus and is required for their nuclear export and maturation.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- MISCELLANEOUS: The C.neoformans mating-type locus is unique, spans >100
CC       kb, and contains more than 20 genes. MAT-encoded products include
CC       homologs of regulators of sexual development in other fungi, pheromone
CC       and pheromone receptors, divergent components of a MAP kinase cascade,
CC       and other proteins with no obvious function in mating.
CC   -!- SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU01058}.
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DR   EMBL; AF542529; AAN75166.2; -; Genomic_DNA.
DR   EMBL; CP003824; AFR94694.2; -; Genomic_DNA.
DR   RefSeq; XP_012048928.1; XM_012193538.1.
DR   AlphaFoldDB; J9VQ03; -.
DR   SMR; J9VQ03; -.
DR   EnsemblFungi; AFR94694; AFR94694; CNAG_07413.
DR   GeneID; 23890260; -.
DR   VEuPathDB; FungiDB:CNAG_07413; -.
DR   HOGENOM; CLU_011106_4_1_1; -.
DR   Proteomes; UP000010091; Chromosome 5.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1580.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR030378; G_CP_dom.
DR   InterPro; IPR006073; GTP-bd.
DR   InterPro; IPR023179; GTP-bd_ortho_bundle_sf.
DR   InterPro; IPR024929; NOG2.
DR   InterPro; IPR012971; NOG2_N_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11089:SF9; PTHR11089:SF9; 1.
DR   Pfam; PF01926; MMR_HSR1; 1.
DR   Pfam; PF08153; NGP1NT; 1.
DR   PRINTS; PR00326; GTP1OBG.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51721; G_CP; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Nucleotide-binding; Nucleus; Ribosome biogenesis.
FT   CHAIN           1..720
FT                   /note="Nucleolar GTP-binding protein 2"
FT                   /id="PRO_0000421127"
FT   DOMAIN          225..386
FT                   /note="CP-type G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          130..162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          488..527
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          568..720
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..15
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        508..527
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        568..613
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        621..644
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        645..669
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        670..687
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        688..720
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         335..342
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   BINDING         379..383
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   720 AA;  80005 MW;  8FDA1749D09F0C1D CRC64;
     MGKGKNHDRK ANPGFGKVKS KSGTSTGEFT LKRVKGENFY RDAKSASRVK MLNGGKAVRD
     RDGKIVEAAA FQKGEKEAEP GRVKPDRRWF GNTRVISQSA LDHFRTALKE QKADPYSVLL
     KRNKLPMGLL QDDTKDGGRR PHIVETEPFG NTFGPKAQRK RPRLDIGSIE ELGESSAASA
     AAAAAETATA ESQGNGTADL ADIYHPTTST AREPIYAKGT SRRIWGELYK VLDSSDVVIH
     VLDARDPLGT RCKPVVEYLR KEKAHKHLVY VLNKVDLVPT WVTARWVKHL SLSAPTIAFH
     ASINNSFGKG SLIQLLRQFS VLHSDKKQIS VGFIGYPNTG KSSIINTLKK KKVCTVAPIP
     GETKVWQYIT LMRRIYLIDC PGIVPVSAKD SDTDTVLKGV VRVENLATPA EHIPALLERV
     RPEYLERTYG LEHVEGGWHG EEGATFVLTA IAKKSGKLLK GGEPDQEAAA KMVLNDWIRG
     KVPFFVAPPT KPESGADAHV SSATTEKVQE QEQKELAEEK ETKEMLEEQE RSLGKVLGIK
     RVKGVEQPIS KIVTMTKFLG DDARRYVEEE EVDVDDVDKE MAEEDEDEDE DDDDDVEESG
     EDQEEEELAW DDVFPEEAAG VDAADGEEVE EDDEEEEGDE DDEDVPSAKQ LGKRKAIDSD
     EEEQTAIKAK RMTTNKQKAS NFYTHANVKN RNRDRKVPKN PGKRSRGDDE PTGKKAKKRR