NOI4_ARATH
ID NOI4_ARATH Reviewed; 79 AA.
AC O22633; F4K693; F4K694; Q9M3R9;
DT 25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Protein NOI4 {ECO:0000303|PubMed:15845764};
DE AltName: Full=Protein DiDi 18T-1d {ECO:0000303|PubMed:11277426};
GN Name=NOI4 {ECO:0000303|PubMed:15845764};
GN OrderedLocusNames=At5g55850 {ECO:0000312|Araport:AT5G55850};
GN ORFNames=MWJ3.3 {ECO:0000312|EMBL:AED96689.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND INDUCTION BY
RP MELOIDOGYNE INCOGNITA.
RC STRAIN=cv. Columbia; TISSUE=Root;
RX PubMed=11277426; DOI=10.1094/mpmi.2001.14.3.288;
RA Vercauteren I., van der Schueren E., Van Montagu M., Gheysen G.;
RT "Arabidopsis thaliana genes expressed in the early compatible interaction
RT with root-knot nematodes.";
RL Mol. Plant Microbe Interact. 14:288-299(2001).
RN [5]
RP MUTAGENESIS OF 11-PHE-GLY-12, AND PROTEOLYTIC CLEAVAGE BY P.SYRINGAE PV
RP TOMATO AVRRPT2.
RX PubMed=15684089; DOI=10.1073/pnas.0409468102;
RA Chisholm S.T., Dahlbeck D., Krishnamurthy N., Day B., Sjolander K.,
RA Staskawicz B.J.;
RT "Molecular characterization of proteolytic cleavage sites of the
RT Pseudomonas syringae effector AvrRpt2.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:2087-2092(2005).
RN [6]
RP NOMENCLATURE.
RX PubMed=15845764; DOI=10.1073/pnas.0500792102;
RA Kim H.-S., Desveaux D., Singer A.U., Patel P., Sondek J., Dangl J.L.;
RT "The Pseudomonas syringae effector AvrRpt2 cleaves its C-terminally
RT acylated target, RIN4, from Arabidopsis membranes to block RPM1
RT activation.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:6496-6501(2005).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O22633-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O22633-2; Sequence=VSP_059535;
CC -!- INDUCTION: During compatible interaction with the endoparasitic
CC nematode M.incognita. {ECO:0000269|PubMed:11277426}.
CC -!- PTM: Proteolytic cleaved by P.syringae pv tomato AvrRpt2 after Gly-12;
CC this cleavage is critical for subsequent proteasome-dependent
CC elimination. {ECO:0000269|PubMed:15684089}.
CC -!- SIMILARITY: Belongs to the RIN4 family. {ECO:0000305}.
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DR EMBL; AB018120; BAA97284.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96687.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96689.2; -; Genomic_DNA.
DR EMBL; AF030386; AAB86938.1; -; mRNA.
DR EMBL; AY065260; AAL38736.1; -; mRNA.
DR EMBL; AY096671; AAM20305.1; -; mRNA.
DR EMBL; AJ286356; CAB71020.1; -; mRNA.
DR RefSeq; NP_001318805.1; NM_001345164.1. [O22633-1]
DR RefSeq; NP_200396.2; NM_124967.3. [O22633-2]
DR AlphaFoldDB; O22633; -.
DR iPTMnet; O22633; -.
DR PRIDE; O22633; -.
DR ProteomicsDB; 249125; -. [O22633-1]
DR EnsemblPlants; AT5G55850.1; AT5G55850.1; AT5G55850. [O22633-2]
DR EnsemblPlants; AT5G55850.3; AT5G55850.3; AT5G55850. [O22633-1]
DR GeneID; 835679; -.
DR Gramene; AT5G55850.1; AT5G55850.1; AT5G55850. [O22633-2]
DR Gramene; AT5G55850.3; AT5G55850.3; AT5G55850. [O22633-1]
DR KEGG; ath:AT5G55850; -.
DR Araport; AT5G55850; -.
DR HOGENOM; CLU_169219_1_0_1; -.
DR OMA; FLMMETQ; -.
DR PhylomeDB; O22633; -.
DR PRO; PR:O22633; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O22633; baseline and differential.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0009624; P:response to nematode; IEP:UniProtKB.
DR InterPro; IPR040387; RIN4/NOI4.
DR InterPro; IPR008700; TypeIII_avirulence_cleave.
DR PANTHER; PTHR33159; PTHR33159; 1.
DR Pfam; PF05627; AvrRpt-cleavage; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..79
FT /note="Protein NOI4"
FT /id="PRO_0000443958"
FT REGION 31..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 12..13
FT /note="Cleavage; by AvrRpt2"
FT /evidence="ECO:0000269|PubMed:15684089"
FT MOD_RES 44
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT VAR_SEQ 66..79
FT /note="KKWLCCMQAPAVDS -> VKKMALLHASSSCGLLTDTKMDLLAAKKKVTVLT
FT HLKAMVLFMFYCRLS (in isoform 2)"
FT /id="VSP_059535"
FT MUTAGEN 11..12
FT /note="FG->AA: Altered P.syringae pv tomato AvrRpt2-
FT mediated cleavage leading to abnormal proteasome-dependent
FT elimination."
FT /evidence="ECO:0000269|PubMed:15684089"
SQ SEQUENCE 79 AA; 8343 MW; F0CC71020825F244 CRC64;
MSDKGRPLPK FGEWDVNDPA SAEGFTVIFN KARDEKKTGG KPGSPGKSSE GHVKSGGGDP
SKPQPKKWLC CMQAPAVDS
