NOI_DROME
ID NOI_DROME Reviewed; 503 AA.
AC O46106;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Splicing factor 3A subunit 3;
DE AltName: Full=Protein noisette;
GN Name=noi; ORFNames=CG2925;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, AND DISRUPTION
RP PHENOTYPE.
RC TISSUE=Embryo, and Ovary;
RX PubMed=9528755; DOI=10.1128/mcb.18.4.1835;
RA Meyer V., Oliver B., Pauli D.;
RT "Multiple developmental requirements of noisette, the Drosophila homolog of
RT the U2 snRNP-associated polypeptide SP3a60.";
RL Mol. Cell. Biol. 18:1835-1843(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360; SER-365 AND SER-367, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Probable subunit of a splicing factor complex required for
CC 'A' complex assembly formed by the stable binding of U2 snRNP to the
CC branchpoint sequence (BPS) in pre-mRNA (By similarity). Involved in
CC male fertility. {ECO:0000250}.
CC -!- SUBUNIT: Probable component of a the U2 small nuclear
CC ribonucleoproteins complex (U2 snRNP). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Note=Excluded from the nucleoli.
CC -!- TISSUE SPECIFICITY: Ubiquitous. In ovaries and testes, it is expressed
CC in all germ and somatic cells. Highly expressed in spermatogonias and
CC spermatocytes. Highly expressed in the germ cells of larval testes,
CC while it is weakly expressed in fat body cells, in polyploid nuclei of
CC salivary glands, and in larval brain.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout embryogenesis from syncytial
CC blastoderm stage.
CC -!- DISRUPTION PHENOTYPE: 'Noisette' means 'hazel nut' in French, and is
CC due to the small size of testes in mutants.
CC {ECO:0000269|PubMed:9528755}.
CC -!- SIMILARITY: Belongs to the SF3A3 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ223042; CAA11045.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF51999.1; -; Genomic_DNA.
DR EMBL; AY051676; AAK93100.1; -; mRNA.
DR RefSeq; NP_477114.1; NM_057766.5.
DR AlphaFoldDB; O46106; -.
DR SMR; O46106; -.
DR BioGRID; 65884; 32.
DR DIP; DIP-19450N; -.
DR IntAct; O46106; 6.
DR STRING; 7227.FBpp0078400; -.
DR iPTMnet; O46106; -.
DR PaxDb; O46106; -.
DR PRIDE; O46106; -.
DR DNASU; 40678; -.
DR EnsemblMetazoa; FBtr0078751; FBpp0078400; FBgn0014366.
DR GeneID; 40678; -.
DR KEGG; dme:Dmel_CG2925; -.
DR UCSC; CG2925-RA; d. melanogaster.
DR CTD; 40678; -.
DR FlyBase; FBgn0014366; noi.
DR VEuPathDB; VectorBase:FBgn0014366; -.
DR eggNOG; KOG2636; Eukaryota.
DR GeneTree; ENSGT00530000063402; -.
DR HOGENOM; CLU_027160_2_0_1; -.
DR InParanoid; O46106; -.
DR OMA; KDAHRRN; -.
DR OrthoDB; 383503at2759; -.
DR PhylomeDB; O46106; -.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR SignaLink; O46106; -.
DR BioGRID-ORCS; 40678; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 40678; -.
DR PRO; PR:O46106; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0014366; Expressed in eye disc (Drosophila) and 23 other tissues.
DR Genevisible; O46106; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0005686; C:U2 snRNP; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; ISS:FlyBase.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009566; P:fertilization; IMP:UniProtKB.
DR GO; GO:0000278; P:mitotic cell cycle; HMP:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:FlyBase.
DR InterPro; IPR000690; Matrin/U1-C_Znf_C2H2.
DR InterPro; IPR031776; SF3A3.
DR InterPro; IPR031774; SF3A3_dom.
DR InterPro; IPR024598; SF3a60/Prp9_C.
DR InterPro; IPR021966; SF3a60_bindingd.
DR PANTHER; PTHR12786:SF3; PTHR12786:SF3; 1.
DR Pfam; PF16837; SF3A3; 1.
DR Pfam; PF12108; SF3a60_bindingd; 1.
DR Pfam; PF11931; SF3a60_Prp9_C; 1.
DR PROSITE; PS50171; ZF_MATRIN; 1.
PE 1: Evidence at protein level;
KW Metal-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Spliceosome; Zinc; Zinc-finger.
FT CHAIN 1..503
FT /note="Splicing factor 3A subunit 3"
FT /id="PRO_0000174320"
FT ZN_FING 408..439
FT /note="Matrin-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00130"
FT REGION 296..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 341..384
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..360
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 361..375
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 360
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 365
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 367
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 503 AA; 58422 MW; 20F3C259C41DE406 CRC64;
METLLEQQRR LHEERERLVK LMVDEHATKK PGEKERIHSE HRLKYLMELH HNSTSQLRDL
YEDKDNERKA EIAALSGPNE FNEFYARLKQ IKQFYKSHPA EVSVPLSVEF DEMIRVYNNP
DDMSALVEFT DEEGGGRYLD LNECYELYLN LRSVEKLDYI TYLMSFDHVF DIPRERKNRE
YRIYIETLND YLHHFILRIQ PLLDLEGELL KVELDFQRQW LMGTFPGFSI KETESALANT
GAHLDLSAFS SWEELASLGL DRLKSALVAL GLKCGGTLEE RAQRLFSTKG KSTLDPALMA
KKPSAKTASA QSREHERHKE IAQLEALLYK YADLLSEQRA ATKENVQRKQ ARTGGERDDS
DVEASESDNE DDPDADDVPY NPKNLPLGWD GKPIPYWLYK LHGLNISYNC EICGNFTYKG
PKAFQRHFAE WRHAHGMRCL GIPNTAHFAN VTQIEDAITL WEKLKSQKQS ERWVADQEEE
FEDSLGNVVN RKTFEDLKRQ GLL
