NOL12_MOUSE
ID NOL12_MOUSE Reviewed; 217 AA.
AC Q8BG17; Q91VI1;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Nucleolar protein 12;
DE AltName: Full=Nucleolar protein of 25 kDa;
GN Name=Nol12; Synonyms=Nop25;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=BALB/cJ;
RX PubMed=16430885; DOI=10.1016/j.yexcr.2005.12.017;
RA Suzuki S., Kanno M., Fujiwara T., Sugiyama H., Yokoyama A., Takahashi H.,
RA Tanaka J.;
RT "Molecular cloning and characterization of Nop25, a novel nucleolar RNA
RT binding protein, highly conserved in vertebrate species.";
RL Exp. Cell Res. 312:1031-1041(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Placenta, and Vagina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May bind to 28S rRNA. In vitro binds single-stranded nucleic
CC acids. {ECO:0000269|PubMed:16430885}.
CC -!- SUBUNIT: Interacts with KIAA1191. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:16430885}.
CC -!- TISSUE SPECIFICITY: Expressed in brain, lung, spleen, kidney and heart.
CC {ECO:0000269|PubMed:16430885}.
CC -!- DEVELOPMENTAL STAGE: Continuously expressed during embryogenesis.
CC {ECO:0000269|PubMed:16430885}.
CC -!- SIMILARITY: Belongs to the RRP17 family. {ECO:0000305}.
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DR EMBL; AY940114; AAX32893.1; -; mRNA.
DR EMBL; AK037035; BAC29680.1; -; mRNA.
DR EMBL; AK083539; BAC38946.1; -; mRNA.
DR EMBL; AK167446; BAE39532.1; -; mRNA.
DR EMBL; BC013701; AAH13701.1; -; mRNA.
DR EMBL; BC094424; AAH94424.1; -; mRNA.
DR CCDS; CCDS27629.1; -.
DR RefSeq; NP_598561.2; NM_133800.3.
DR AlphaFoldDB; Q8BG17; -.
DR SMR; Q8BG17; -.
DR BioGRID; 220934; 1.
DR IntAct; Q8BG17; 1.
DR STRING; 10090.ENSMUSP00000116103; -.
DR iPTMnet; Q8BG17; -.
DR PhosphoSitePlus; Q8BG17; -.
DR EPD; Q8BG17; -.
DR MaxQB; Q8BG17; -.
DR PaxDb; Q8BG17; -.
DR PeptideAtlas; Q8BG17; -.
DR PRIDE; Q8BG17; -.
DR ProteomicsDB; 252918; -.
DR DNASU; 97961; -.
DR Ensembl; ENSMUST00000138880; ENSMUSP00000116103; ENSMUSG00000033099.
DR GeneID; 97961; -.
DR KEGG; mmu:97961; -.
DR UCSC; uc007wrw.1; mouse.
DR CTD; 79159; -.
DR MGI; MGI:2146285; Nol12.
DR VEuPathDB; HostDB:ENSMUSG00000033099; -.
DR eggNOG; KOG4709; Eukaryota.
DR GeneTree; ENSGT00390000015973; -.
DR HOGENOM; CLU_111183_0_0_1; -.
DR InParanoid; Q8BG17; -.
DR OMA; HKEYVKM; -.
DR OrthoDB; 1635966at2759; -.
DR PhylomeDB; Q8BG17; -.
DR TreeFam; TF323855; -.
DR BioGRID-ORCS; 97961; 16 hits in 72 CRISPR screens.
DR ChiTaRS; Nol12; mouse.
DR PRO; PR:Q8BG17; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8BG17; protein.
DR Bgee; ENSMUSG00000033099; Expressed in dorsal pancreas and 248 other tissues.
DR ExpressionAtlas; Q8BG17; baseline and differential.
DR Genevisible; Q8BG17; MM.
DR GO; GO:0005730; C:nucleolus; IDA:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; ISO:MGI.
DR GO; GO:0019843; F:rRNA binding; ISO:MGI.
DR GO; GO:0003697; F:single-stranded DNA binding; ISO:MGI.
DR InterPro; IPR019186; Nucleolar_protein_12.
DR PANTHER; PTHR14577; PTHR14577; 1.
DR Pfam; PF09805; Nop25; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; RNA-binding; rRNA-binding.
FT CHAIN 1..217
FT /note="Nucleolar protein 12"
FT /id="PRO_0000271208"
FT REGION 122..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 34..98
FT /evidence="ECO:0000255"
FT COMPBIAS 138..152
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 172..187
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 29
FT /note="R -> P (in Ref. 3; AAH13701)"
FT /evidence="ECO:0000305"
FT CONFLICT 92
FT /note="A -> V (in Ref. 3; AAH13701)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 217 AA; 25356 MW; D2CB0564FE6490A7 CRC64;
MGRNKKKKKR DGDDRRPRLV LNFDEEKRRE YLTGFHKRKV ERKKAAIEEI KQRLKQEQKK
LREERHQEYL KMLAEREEAL EEADELERLV TAKTESVQYD HPNHTVTVTT ISDLDLSGAR
LLGLPLPEQG DQDGSQEEEM SSLEKPTKAL PRKSKDPLLS QRISSLTATL HAHSRKKVKR
KHPRRAQDST KKPPSATRTS KTQRRRRMTG KARHNGE
