NOL4_HUMAN
ID NOL4_HUMAN Reviewed; 638 AA.
AC O94818; B4DSQ0; B7Z3Z7; F5H1E3; Q6IBS2; Q9BWF1;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Nucleolar protein 4;
DE AltName: Full=Nucleolar-localized protein;
GN Name=NOL4; Synonyms=NOLP; ORFNames=HRIHFB2255;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Fetal brain;
RX PubMed=9813152; DOI=10.1006/bbrc.1998.9606;
RA Ueki N., Kondo M., Seki N., Yano K., Oda T., Masuho Y., Muramatsu M.-A.;
RT "NOLP: identification of a novel human nucleolar protein and determination
RT of sequence requirements for its nucleolar localization.";
RL Biochem. Biophys. Res. Commun. 252:97-102(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
RC TISSUE=Brain, and Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16177791; DOI=10.1038/nature03983;
RA Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D.,
RA Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J.,
RA Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L.,
RA Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A.,
RA Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C.,
RA Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 18.";
RL Nature 437:551-555(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-638 (ISOFORM 2).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 115-638 (ISOFORM 2).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 115-638 (ISOFORM 2).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 336-638 (ISOFORM 1), AND
RP SUBCELLULAR LOCATION.
RC TISSUE=Fetal brain;
RX PubMed=9853615; DOI=10.1038/4315;
RA Ueki N., Oda T., Kondo M., Yano K., Noguchi T., Muramatsu M.-A.;
RT "Selection system for genes encoding nuclear-targeted proteins.";
RL Nat. Biotechnol. 16:1338-1342(1998).
CC -!- INTERACTION:
CC O94818-2; Q9BXJ5: C1QTNF2; NbExp=3; IntAct=EBI-10190763, EBI-2817707;
CC O94818-2; Q13363-2: CTBP1; NbExp=4; IntAct=EBI-10190763, EBI-10171858;
CC O94818-2; P56545: CTBP2; NbExp=3; IntAct=EBI-10190763, EBI-741533;
CC O94818-2; P56545-3: CTBP2; NbExp=3; IntAct=EBI-10190763, EBI-10171902;
CC O94818-2; Q8IZU0: FAM9B; NbExp=3; IntAct=EBI-10190763, EBI-10175124;
CC O94818-2; P43364: MAGEA11; NbExp=3; IntAct=EBI-10190763, EBI-739552;
CC O94818-2; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-10190763, EBI-16439278;
CC O94818-2; P07237: P4HB; NbExp=3; IntAct=EBI-10190763, EBI-395883;
CC O94818-2; Q8IX90: SKA3; NbExp=3; IntAct=EBI-10190763, EBI-1773976;
CC O94818-2; O75971: SNAPC5; NbExp=3; IntAct=EBI-10190763, EBI-749483;
CC O94818-2; O75971-2: SNAPC5; NbExp=3; IntAct=EBI-10190763, EBI-12004298;
CC O94818-2; Q8N6V9: TEX9; NbExp=6; IntAct=EBI-10190763, EBI-746341;
CC O94818-2; Q8NA42: ZNF383; NbExp=3; IntAct=EBI-10190763, EBI-20110775;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:9813152,
CC ECO:0000269|PubMed:9853615}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=O94818-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O94818-2; Sequence=VSP_010080;
CC Name=3;
CC IsoId=O94818-3; Sequence=VSP_043344;
CC Name=4;
CC IsoId=O94818-4; Sequence=VSP_045836;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in fetal brain, adult brain
CC and testis. {ECO:0000269|PubMed:9813152}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH00313.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the N-terminal part.; Evidence={ECO:0000305};
CC Sequence=BAA34576.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA34576.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB017800; BAA34576.1; ALT_SEQ; mRNA.
DR EMBL; AK296539; BAH12383.1; -; mRNA.
DR EMBL; AK299850; BAG61712.1; -; mRNA.
DR EMBL; AC010798; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC018972; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC087397; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC104985; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC000313; AAH00313.1; ALT_SEQ; mRNA.
DR EMBL; BT006763; AAP35409.1; -; mRNA.
DR EMBL; CR456730; CAG33011.1; -; mRNA.
DR EMBL; AB015339; BAA34797.1; -; mRNA.
DR CCDS; CCDS11907.2; -. [O94818-1]
DR CCDS; CCDS56058.1; -. [O94818-4]
DR CCDS; CCDS56059.1; -. [O94818-3]
DR CCDS; CCDS59308.1; -. [O94818-2]
DR PIR; JE0335; JE0335.
DR RefSeq; NP_001185475.1; NM_001198546.1.
DR RefSeq; NP_001185476.1; NM_001198547.1. [O94818-3]
DR RefSeq; NP_001185477.1; NM_001198548.1. [O94818-2]
DR RefSeq; NP_001185478.1; NM_001198549.1. [O94818-4]
DR RefSeq; NP_001269456.1; NM_001282527.1.
DR RefSeq; NP_003778.2; NM_003787.4. [O94818-1]
DR RefSeq; XP_006722626.1; XM_006722563.2. [O94818-1]
DR RefSeq; XP_011524539.1; XM_011526237.1. [O94818-1]
DR RefSeq; XP_011524540.1; XM_011526238.1.
DR RefSeq; XP_016881548.1; XM_017026059.1. [O94818-4]
DR AlphaFoldDB; O94818; -.
DR BioGRID; 114256; 50.
DR IntAct; O94818; 18.
DR MINT; O94818; -.
DR STRING; 9606.ENSP00000261592; -.
DR iPTMnet; O94818; -.
DR PhosphoSitePlus; O94818; -.
DR BioMuta; NOL4; -.
DR EPD; O94818; -.
DR jPOST; O94818; -.
DR MassIVE; O94818; -.
DR MaxQB; O94818; -.
DR PaxDb; O94818; -.
DR PeptideAtlas; O94818; -.
DR PRIDE; O94818; -.
DR ProteomicsDB; 25628; -.
DR ProteomicsDB; 50459; -. [O94818-1]
DR ProteomicsDB; 50460; -. [O94818-2]
DR ProteomicsDB; 50461; -. [O94818-3]
DR Antibodypedia; 8389; 123 antibodies from 25 providers.
DR DNASU; 8715; -.
DR Ensembl; ENST00000261592.10; ENSP00000261592.4; ENSG00000101746.16. [O94818-1]
DR Ensembl; ENST00000535384.5; ENSP00000445733.1; ENSG00000101746.16. [O94818-4]
DR Ensembl; ENST00000538587.5; ENSP00000443472.1; ENSG00000101746.16. [O94818-3]
DR Ensembl; ENST00000589544.5; ENSP00000465450.1; ENSG00000101746.16. [O94818-2]
DR GeneID; 8715; -.
DR KEGG; hsa:8715; -.
DR MANE-Select; ENST00000261592.10; ENSP00000261592.4; NM_003787.5; NP_003778.2.
DR UCSC; uc002kxt.5; human. [O94818-1]
DR CTD; 8715; -.
DR DisGeNET; 8715; -.
DR GeneCards; NOL4; -.
DR HGNC; HGNC:7870; NOL4.
DR HPA; ENSG00000101746; Tissue enhanced (brain, pituitary gland, retina, testis).
DR MIM; 603577; gene.
DR neXtProt; NX_O94818; -.
DR OpenTargets; ENSG00000101746; -.
DR PharmGKB; PA31674; -.
DR VEuPathDB; HostDB:ENSG00000101746; -.
DR eggNOG; ENOG502QR3R; Eukaryota.
DR GeneTree; ENSGT00940000159992; -.
DR HOGENOM; CLU_020587_0_0_1; -.
DR InParanoid; O94818; -.
DR OMA; VPADKQC; -.
DR OrthoDB; 1009964at2759; -.
DR PhylomeDB; O94818; -.
DR TreeFam; TF325594; -.
DR PathwayCommons; O94818; -.
DR SignaLink; O94818; -.
DR BioGRID-ORCS; 8715; 6 hits in 1071 CRISPR screens.
DR ChiTaRS; NOL4; human.
DR GenomeRNAi; 8715; -.
DR Pharos; O94818; Tbio.
DR PRO; PR:O94818; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; O94818; protein.
DR Bgee; ENSG00000101746; Expressed in cortical plate and 132 other tissues.
DR ExpressionAtlas; O94818; baseline and differential.
DR Genevisible; O94818; HS.
DR GO; GO:0005730; C:nucleolus; TAS:ProtInc.
DR GO; GO:0003723; F:RNA binding; TAS:ProtInc.
DR InterPro; IPR026747; NOL4.
DR InterPro; IPR039788; NOL4/NOL4L.
DR PANTHER; PTHR12449; PTHR12449; 1.
DR PANTHER; PTHR12449:SF17; PTHR12449:SF17; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome.
FT CHAIN 1..638
FT /note="Nucleolar protein 4"
FT /id="PRO_0000096935"
FT REGION 210..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 343..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 503..535
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 573..603
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 227..286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 296..312
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..366
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 503..522
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..285
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_045836"
FT VAR_SEQ 1..87
FT /note="MESERDMYRQFQDWCLRTYGDSGKTKTVTRKKYERIVQLLNGSESSSTDNAK
FT FKFWVKSKGFQLGQPDEVRGGGGGAKQVLYVPVKT -> MADLMQETFLHHA (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_043344"
FT VAR_SEQ 413..514
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.5,
FT ECO:0000303|Ref.6"
FT /id="VSP_010080"
FT CONFLICT 637
FT /note="Q -> H (in Ref. 2; BAH12383)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 638 AA; 71357 MW; 02DF0130F10E0B3F CRC64;
MESERDMYRQ FQDWCLRTYG DSGKTKTVTR KKYERIVQLL NGSESSSTDN AKFKFWVKSK
GFQLGQPDEV RGGGGGAKQV LYVPVKTTDG VGVDEKLSLR RVAVVEDFFD IIYSMHVETG
PNGEQIRKHA GQKRTYKAIS ESYAFLPREA VTRFLMSCSE CQKRMHLNPD GTDHKDNGKP
PTLVTSMIDY NMPITMAYMK HMKLQLLNSQ QDEDESSIES DEFDMSDSTR MSAVNSDLSS
NLEERMQSPQ NLHGQQDDDS AAESFNGNET LGHSSIASGG THSREMGDSN SDGKTGLEQD
EQPLNLSDSP LSAQLTSEYR IDDHNSNGKN KYKNLLISDL KMEREARENG SKSPAHSYSS
YDSGKNESVD RGAEDLSLNR GDEDEDDHED HDDSEKVNET DGVEAERLKA FNMFVRLFVD
ENLDRMVPIS KQPKEKIQAI IDSCRRQFPE YQERARKRIR TYLKSCRRMK RSGFEMSRPI
PSHLTSAVAE SILASACESE SRNAAKRMRL ERQQDESAPA DKQCKPEATQ ATYSTSAVPG
SQDVLYINGN GTYSYHSYRG LGGGLLNLND ASSSGPTDLS MKRQLATSSG SSSSSNSRPQ
LSPTEINAVR QLVAGYRESA AFLLRSADEL ENLILQQN
