NOL6_DROME
ID NOL6_DROME Reviewed; 1193 AA.
AC Q8IH00; Q9VEX3;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Nucleolar protein 6 {ECO:0000250|UniProtKB:Q9H6R4};
DE AltName: Full=Maternal transcript 89Ba {ECO:0000303|PubMed:9799434};
GN Name=Mat89Ba {ECO:0000303|PubMed:9799434}; ORFNames=CG12785;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF55292.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAF55292.2}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAN71266.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAN71266.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:AAV36998.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAV36998.1}; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1094-1193, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=9799434; DOI=10.1007/s004270050211;
RA Stebbings L.A., Grimes B.R., Bownes M.;
RT "A testis-specifically expressed gene is embedded within a cluster of
RT maternally expressed genes at 89B in Drosophila melanogaster.";
RL Dev. Genes Evol. 208:523-530(1998).
RN [6] {ECO:0000305}
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo {ECO:0000269|PubMed:18327897};
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- SUBUNIT: May be associated with rRNA. {ECO:0000250|UniProtKB:Q9H6R4}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q8R5K4}.
CC -!- TISSUE SPECIFICITY: Expressed in nurse cells at stages 9-10 of
CC oogenesis and exported to the oocyte. {ECO:0000269|PubMed:9799434}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:9799434}.
CC -!- SIMILARITY: Belongs to the NRAP family. {ECO:0000255}.
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DR EMBL; AE014297; AAF55292.2; -; Genomic_DNA.
DR EMBL; BT001511; AAN71266.1; -; mRNA.
DR EMBL; BT016113; AAV36998.1; -; mRNA.
DR EMBL; U47618; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_650530.2; NM_142273.4.
DR AlphaFoldDB; Q8IH00; -.
DR SMR; Q8IH00; -.
DR BioGRID; 67021; 4.
DR IntAct; Q8IH00; 8.
DR STRING; 7227.FBpp0082727; -.
DR iPTMnet; Q8IH00; -.
DR PaxDb; Q8IH00; -.
DR PRIDE; Q8IH00; -.
DR DNASU; 41973; -.
DR EnsemblMetazoa; FBtr0083275; FBpp0082727; FBgn0261286.
DR GeneID; 41973; -.
DR KEGG; dme:Dmel_CG12785; -.
DR UCSC; CG12785-RA; d. melanogaster.
DR CTD; 41973; -.
DR FlyBase; FBgn0261286; Mat89Ba.
DR VEuPathDB; VectorBase:FBgn0261286; -.
DR eggNOG; KOG2054; Eukaryota.
DR GeneTree; ENSGT00390000018619; -.
DR HOGENOM; CLU_003502_0_1_1; -.
DR InParanoid; Q8IH00; -.
DR OMA; NPHGGKE; -.
DR OrthoDB; 176625at2759; -.
DR PhylomeDB; Q8IH00; -.
DR Reactome; R-DME-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR SignaLink; Q8IH00; -.
DR BioGRID-ORCS; 41973; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41973; -.
DR PRO; PR:Q8IH00; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0261286; Expressed in egg chamber and 37 other tissues.
DR Genevisible; Q8IH00; DM.
DR GO; GO:0000794; C:condensed nuclear chromosome; ISS:UniProtKB.
DR GO; GO:0032545; C:CURI complex; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; ISS:FlyBase.
DR GO; GO:0032040; C:small-subunit processome; IBA:GO_Central.
DR GO; GO:0034456; C:UTP-C complex; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; ISS:FlyBase.
DR GO; GO:0006364; P:rRNA processing; ISS:FlyBase.
DR GO; GO:0006409; P:tRNA export from nucleus; IBA:GO_Central.
DR InterPro; IPR005554; NOL6/Upt22.
DR InterPro; IPR035082; Nrap_D1.
DR InterPro; IPR035367; Nrap_D2.
DR InterPro; IPR035368; Nrap_D3.
DR InterPro; IPR035369; Nrap_D4.
DR InterPro; IPR035370; Nrap_D5.
DR InterPro; IPR035371; Nrap_D6.
DR PANTHER; PTHR17972; PTHR17972; 1.
DR Pfam; PF03813; Nrap; 1.
DR Pfam; PF17403; Nrap_D2; 1.
DR Pfam; PF17404; Nrap_D3; 1.
DR Pfam; PF17405; Nrap_D4; 1.
DR Pfam; PF17406; Nrap_D5; 1.
DR Pfam; PF17407; Nrap_D6; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; RNA-binding.
FT CHAIN 1..1193
FT /note="Nucleolar protein 6"
FT /id="PRO_0000383624"
FT REGION 1..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1137..1193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1137..1163
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1164..1187
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 35
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 1193 AA; 136385 MW; 48175B8B5280D893 CRC64;
MRFVQGNIMP GKLVGSPKGA ARTGTQAIAH AGDHSDLDEP KPKIAKSDPP STAIPKKNNF
KQREGTKNVK PPTLEEMKEL RDTQNLFHSN LFKLQVKEML EELQLKQKYT DFIENWLESF
TAFTRQLKDG LMERTHLEVP MKLSEKPTGF VFSKPTREPY LIGAAATGTL LGPKIVVDVA
LEMPKESLHK EDYLNLRYDQ KRALYLTYVT ERMMESPDYA EDQFNFNYYA NNPLKPVLEL
IPVTKQVNKH LQVRLFITAP LSSFKPGRFV PWNNNIRPSF YGDEWDEKDP LPSTQHYNAN
VLFDLTLSEN QAQLDKAFKS RRNFQDGLLL LKVWLRQRQL DIGYSGFGAH ILAAFIVYLN
TQRILHQSSS SYQVARTVWN QLANTDWTKG ISLSVAPIQT EELNKFAEHY DVCFIDFTGQ
HNLCANIPLY LYQRVREEAK LAVELLNDMK LNSFPLIFMQ KCPLYSRVDN ILKISNYSCI
NQMLTLHSHP RIKYDFANYG YPQLLHLLTE LLKKGLAERV HSILPLETAT AAWPVEKKAP
VIGKYIQLGL ILQPEHAYQV LNKGPAANDD HEGAEEFRRF WGEKSNLRRF QDGSITEAVV
WGTAQDSPAK KRLIVRQIVL HLLEHHLQLD SKEVQYIGGE LDQVYQLSPW FKVNKLKTKL
PLGQDTDAEA LSPHVIRCYD ELARQLHGLN DLPLEIVSIS GVSPIFRYCE PQPVLPQALL
VENRILASSI QRVVIQLGQS GKWPTELGAL RALKTAFLIE IGEKLEAQCR LHWVMSADGL
LVLKQGYCFL IELAHNKELA LLKQEVTERG ITTYIDNAAS RSLERQHYIL PKVSGALHSL
HQTYSAFGST VLLAKRWLAT QLLDDGLWPD MATELLVAHL FQQRYAPQSI AAPQTGFIRF
LQLLSHSDFN GELFLLNFNN SWQEQQIADL EHNYRSNRQS YPPLAVATSY DMQHAGRLWT
SDQSPSQRVL GHVTRLARHA LEIIETSLMS KDLRFVRPAQ LFRASNEGYD LVIQFKPDLV
PNSLSYDLGS PFVSFSQPNF SLPRAGSDYI ARIVGLLRSA YSDFAAFFYN PHGGKELGIV
WRPPTEFAAK PFKVTELQAC SPCGNKKVQV LKETLLEDFK LLLKDFYLRI ATPEELKREQ
REHQKPMRYF DANKAEEESK PKPKKHRQRK GTGKKALPKR KRLIKSSTLK VLK
