NOL6_MOUSE
ID NOL6_MOUSE Reviewed; 1152 AA.
AC Q8R5K4; Q3TL75; Q3U1P8; Q6PAN8; Q8C6V4; Q8R5K3; Q8VCG0; Q8WTY7;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Nucleolar protein 6;
DE AltName: Full=Nucleolar RNA-associated protein;
DE Short=Nrap;
GN Name=Nol6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=11895476; DOI=10.1046/j.1356-9597.2001.00507.x;
RA Utama B., Kennedy D., Ru K., Mattick J.S.;
RT "Isolation and characterization of a new nucleolar protein, Nrap, that is
RT conserved from yeast to humans.";
RL Genes Cells 7:115-132(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Dendritic cell, Embryo, and Lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, Liver, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probably associated with rRNA.
CC {ECO:0000250|UniProtKB:Q9H6R4}.
CC -!- SUBUNIT: Interacts with RRP7A; required for NOL6 localization to
CC nucleolus. {ECO:0000250|UniProtKB:Q9H6R4}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:11895476}.
CC Chromosome {ECO:0000269|PubMed:11895476}. Note=Localizes to condensed
CC chromosomes in mitosis.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Alpha, Long;
CC IsoId=Q8R5K4-1; Sequence=Displayed;
CC Name=2; Synonyms=Beta, Short;
CC IsoId=Q8R5K4-2; Sequence=VSP_013533, VSP_013534;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:11895476}.
CC -!- SIMILARITY: Belongs to the NRAP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH19981.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH21856.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAL74401.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAL74402.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF361077; AAL74401.1; ALT_INIT; mRNA.
DR EMBL; AF361078; AAL74402.1; ALT_INIT; mRNA.
DR EMBL; AK053117; BAC35271.1; -; mRNA.
DR EMBL; AK155818; BAE33447.1; -; mRNA.
DR EMBL; AK166650; BAE38917.1; -; mRNA.
DR EMBL; AL954379; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC019981; AAH19981.1; ALT_INIT; mRNA.
DR EMBL; BC021856; AAH21856.1; ALT_INIT; mRNA.
DR EMBL; BC059820; AAH59820.2; -; mRNA.
DR CCDS; CCDS38715.1; -. [Q8R5K4-1]
DR RefSeq; NP_631982.2; NM_139236.3. [Q8R5K4-1]
DR AlphaFoldDB; Q8R5K4; -.
DR SMR; Q8R5K4; -.
DR BioGRID; 230928; 4.
DR IntAct; Q8R5K4; 1.
DR STRING; 10090.ENSMUSP00000030138; -.
DR iPTMnet; Q8R5K4; -.
DR PhosphoSitePlus; Q8R5K4; -.
DR EPD; Q8R5K4; -.
DR MaxQB; Q8R5K4; -.
DR PaxDb; Q8R5K4; -.
DR PeptideAtlas; Q8R5K4; -.
DR PRIDE; Q8R5K4; -.
DR ProteomicsDB; 293873; -. [Q8R5K4-1]
DR ProteomicsDB; 293874; -. [Q8R5K4-2]
DR Antibodypedia; 25265; 126 antibodies from 22 providers.
DR DNASU; 230082; -.
DR Ensembl; ENSMUST00000030138; ENSMUSP00000030138; ENSMUSG00000028430. [Q8R5K4-1]
DR GeneID; 230082; -.
DR KEGG; mmu:230082; -.
DR UCSC; uc008sii.1; mouse. [Q8R5K4-1]
DR CTD; 65083; -.
DR MGI; MGI:2140151; Nol6.
DR VEuPathDB; HostDB:ENSMUSG00000028430; -.
DR eggNOG; KOG2054; Eukaryota.
DR GeneTree; ENSGT00390000018619; -.
DR HOGENOM; CLU_003502_0_1_1; -.
DR InParanoid; Q8R5K4; -.
DR OMA; NPHGGKE; -.
DR OrthoDB; 176625at2759; -.
DR PhylomeDB; Q8R5K4; -.
DR Reactome; R-MMU-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR BioGRID-ORCS; 230082; 27 hits in 74 CRISPR screens.
DR ChiTaRS; Nol6; mouse.
DR PRO; PR:Q8R5K4; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8R5K4; protein.
DR Bgee; ENSMUSG00000028430; Expressed in primary visual cortex and 216 other tissues.
DR Genevisible; Q8R5K4; MM.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:UniProtKB.
DR GO; GO:0032545; C:CURI complex; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0032040; C:small-subunit processome; IBA:GO_Central.
DR GO; GO:0034456; C:UTP-C complex; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR GO; GO:0006409; P:tRNA export from nucleus; IBA:GO_Central.
DR InterPro; IPR005554; NOL6/Upt22.
DR InterPro; IPR035082; Nrap_D1.
DR InterPro; IPR035367; Nrap_D2.
DR InterPro; IPR035368; Nrap_D3.
DR InterPro; IPR035369; Nrap_D4.
DR InterPro; IPR035370; Nrap_D5.
DR InterPro; IPR035371; Nrap_D6.
DR PANTHER; PTHR17972; PTHR17972; 1.
DR Pfam; PF03813; Nrap; 1.
DR Pfam; PF17403; Nrap_D2; 1.
DR Pfam; PF17404; Nrap_D3; 1.
DR Pfam; PF17405; Nrap_D4; 1.
DR Pfam; PF17406; Nrap_D5; 1.
DR Pfam; PF17407; Nrap_D6; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chromosome; Coiled coil; Nucleus; Phosphoprotein;
KW Reference proteome; RNA-binding.
FT CHAIN 1..1152
FT /note="Nucleolar protein 6"
FT /id="PRO_0000215648"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 36..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 92..128
FT /evidence="ECO:0000255"
FT COMPBIAS 36..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 65
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6R4"
FT MOD_RES 292
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6R4"
FT MOD_RES 817
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H6R4"
FT VAR_SEQ 1062..1065
FT /note="QLYL -> LSLL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11895476"
FT /id="VSP_013533"
FT VAR_SEQ 1066..1152
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11895476"
FT /id="VSP_013534"
FT CONFLICT 254
FT /note="D -> V (in Ref. 2; BAE38917)"
FT /evidence="ECO:0000305"
FT CONFLICT 740
FT /note="P -> L (in Ref. 2; BAE38917)"
FT /evidence="ECO:0000305"
FT CONFLICT 815
FT /note="V -> M (in Ref. 2; BAE38917)"
FT /evidence="ECO:0000305"
FT CONFLICT 1055
FT /note="V -> I (in Ref. 2; BAE38917)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1152 AA; 129228 MW; 187243E352C68C80 CRC64;
MPSAGERPAV KMGPAPAGEQ HRRATEDPEV MELAFEGMDK EKAPSRKRAR TEPPAEGLLQ
PVNLSREELY KEPTNEELNR LRETEILFHS TLLRLQVEEL LKEVRLSEKK KERIDNFLKE
VTKRIQKVPP VPEAELTDQS WLPAGVRVPL HQVPYAVKGS FRFRPPSQIT VVGSYLLDTC
MRPDINVDVA VTMPREILQD KDGLNQRYFR KRALYLAHLA YHLAQDPLFS SVRFSYMSGC
HLKPSLLLRP HGKDERLVTV RLLPCPPLDF FRPCRLLPTK NNVRSAWYRG QSCPDYEPPT
PHYNTWILQD VALETHMHLL ASVLGSAQGL KDGVALLKVW LRQRELDKGL GGFNGFIISM
LVAFLVSKRK IHTTMSGYQV LRSVLQFLAT TDLTINGISF SLSSDPSLPT LAEFHQLFAV
VFVDPSGRLN LCADVTASTY NQVQYEAELS MALLDSKADD GFQLLLMTPK PMIQAFDHVV
HLHPLSRLQA SCHQLKLWPE LQDNGGDYVS AALGPLTNIL VQGLGCRLHL LAHSRPPVPE
WSINQDPPKH KDAGTLTLGF LFRPEGLTSV IDLGPEADKP EAADFRQFWG TRSELRRFQD
GAIREAVVWE AESLFEKRLI PHQVVTHLLA LHADIPDTCI QYVGGFLDAL IQNPKEISST
GEEALALAVR CYDDLSRLLW GLEGLPLTVS AVQGAHPVLR YTEVFPPAPV RPAYSFYNRL
QELASLLPRP DKPCPAYVEP MTVVCHLEGS GQWPQDAEAV QRVRAAFQLR LAEVLTQEHR
LQCCATATHT DVLKDGFVFR IRVAYQREPQ ILKEVRSPEG MVSLRDTPAS LRLERDTKLL
PLLTSALHGL QQQYPAYSGV ARLAKRWVRA QLLGEGFTDE SLDLLAASLF LHPEPFTPPS
VPQVGFLRFL YLVSTFDWKN NPLIVNLNGE LTAEEQVGIR SSFLAARTQL PVMVIITPQD
RRSSVWTQDG PSAQILQQLV SLAAEALPIL EKQLMDPRGP GDIRTVFRPP FDMYDVLIHL
TPRHIPRHRQ AVDPPVASFC RGLLAEPGPS SLMPVLGYDP PQLYLAQLRE AFEDLALFFY
DQHGGEVIGV LWKPSSFQPQ PFKASSIKGR MVVSQGGELV MLPNIEAILE DFAVLGEGLV
QAVEARSERW TV
