NOL7_HUMAN
ID NOL7_HUMAN Reviewed; 257 AA.
AC Q9UMY1; Q5T297; Q9Y3U7;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Nucleolar protein 7;
DE AltName: Full=Nucleolar protein of 27 kDa;
GN Name=NOL7; Synonyms=C6orf90, NOP27;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=16205646; DOI=10.1038/sj.onc.1209070;
RA Hasina R., Pontier A.L., Fekete M.J., Martin L.E., Qi X.M., Brigaudeau C.,
RA Pramanik R., Cline E.I., Coignet L.J., Lingen M.W.;
RT "NOL7 is a nucleolar candidate tumor suppressor gene in cervical cancer
RT that modulates the angiogenic phenotype.";
RL Oncogene 25:588-598(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 91-236 (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=11790298; DOI=10.1016/s0960-9822(01)00650-9;
RA Andersen J.S., Lyon C.E., Fox A.H., Leung A.K.L., Lam Y.W., Steen H.,
RA Mann M., Lamond A.I.;
RT "Directed proteomic analysis of the human nucleolus.";
RL Curr. Biol. 12:1-11(2002).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=12429849; DOI=10.1091/mbc.e02-05-0271;
RA Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA Greco A., Hochstrasser D.F., Diaz J.-J.;
RT "Functional proteomic analysis of human nucleolus.";
RL Mol. Biol. Cell 13:4100-4109(2002).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [9]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-131 AND LYS-162, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- INTERACTION:
CC Q9UMY1; O95273: CCNDBP1; NbExp=3; IntAct=EBI-2862609, EBI-748961;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:11790298,
CC ECO:0000269|PubMed:12429849, ECO:0000269|PubMed:16205646}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9UMY1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9UMY1-2; Sequence=VSP_014791, VSP_014792;
CC -!- TISSUE SPECIFICITY: Expressed in numerous tissues. Particularly
CC prevalent in the adrenal gland, thyroid gland, heart and skeletal
CC muscle. {ECO:0000269|PubMed:16205646}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB43398.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR EMBL; AF172066; AAD49745.2; -; mRNA.
DR EMBL; AK075301; BAC11534.1; -; mRNA.
DR EMBL; AL441883; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC023517; AAH23517.1; -; mRNA.
DR EMBL; BC062683; AAH62683.1; -; mRNA.
DR EMBL; AL050299; CAB43398.1; ALT_SEQ; mRNA.
DR CCDS; CCDS4528.1; -. [Q9UMY1-1]
DR PIR; T08718; T08718.
DR RefSeq; NP_001304653.1; NM_001317724.1. [Q9UMY1-1]
DR RefSeq; NP_057251.2; NM_016167.4. [Q9UMY1-1]
DR PDB; 7MQ8; EM; 3.60 A; ND=1-257.
DR PDB; 7MQ9; EM; 3.87 A; ND=1-257.
DR PDB; 7MQA; EM; 2.70 A; ND=1-257.
DR PDBsum; 7MQ8; -.
DR PDBsum; 7MQ9; -.
DR PDBsum; 7MQA; -.
DR AlphaFoldDB; Q9UMY1; -.
DR SMR; Q9UMY1; -.
DR BioGRID; 119526; 53.
DR IntAct; Q9UMY1; 23.
DR MINT; Q9UMY1; -.
DR STRING; 9606.ENSP00000405674; -.
DR iPTMnet; Q9UMY1; -.
DR PhosphoSitePlus; Q9UMY1; -.
DR BioMuta; NOL7; -.
DR DMDM; 71152014; -.
DR SWISS-2DPAGE; Q9UMY1; -.
DR EPD; Q9UMY1; -.
DR jPOST; Q9UMY1; -.
DR MassIVE; Q9UMY1; -.
DR MaxQB; Q9UMY1; -.
DR PaxDb; Q9UMY1; -.
DR PeptideAtlas; Q9UMY1; -.
DR PRIDE; Q9UMY1; -.
DR ProteomicsDB; 85228; -. [Q9UMY1-1]
DR ProteomicsDB; 85229; -. [Q9UMY1-2]
DR TopDownProteomics; Q9UMY1-1; -. [Q9UMY1-1]
DR TopDownProteomics; Q9UMY1-2; -. [Q9UMY1-2]
DR Antibodypedia; 24978; 32 antibodies from 16 providers.
DR DNASU; 51406; -.
DR Ensembl; ENST00000451315.7; ENSP00000405674.2; ENSG00000225921.7. [Q9UMY1-1]
DR GeneID; 51406; -.
DR KEGG; hsa:51406; -.
DR MANE-Select; ENST00000451315.7; ENSP00000405674.2; NM_016167.5; NP_057251.2.
DR UCSC; uc003naz.4; human. [Q9UMY1-1]
DR CTD; 51406; -.
DR DisGeNET; 51406; -.
DR GeneCards; NOL7; -.
DR HGNC; HGNC:21040; NOL7.
DR HPA; ENSG00000225921; Low tissue specificity.
DR MIM; 611533; gene.
DR neXtProt; NX_Q9UMY1; -.
DR OpenTargets; ENSG00000225921; -.
DR PharmGKB; PA134881915; -.
DR VEuPathDB; HostDB:ENSG00000225921; -.
DR eggNOG; ENOG502S14X; Eukaryota.
DR GeneTree; ENSGT00390000004118; -.
DR HOGENOM; CLU_095368_0_0_1; -.
DR InParanoid; Q9UMY1; -.
DR OMA; WACKEKA; -.
DR PhylomeDB; Q9UMY1; -.
DR TreeFam; TF336320; -.
DR PathwayCommons; Q9UMY1; -.
DR SignaLink; Q9UMY1; -.
DR BioGRID-ORCS; 51406; 614 hits in 1081 CRISPR screens.
DR ChiTaRS; NOL7; human.
DR GeneWiki; NOL7; -.
DR GenomeRNAi; 51406; -.
DR Pharos; Q9UMY1; Tbio.
DR PRO; PR:Q9UMY1; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q9UMY1; protein.
DR Bgee; ENSG00000225921; Expressed in parotid gland and 208 other tissues.
DR ExpressionAtlas; Q9UMY1; baseline and differential.
DR Genevisible; Q9UMY1; HS.
DR GO; GO:0005694; C:chromosome; IDA:HPA.
DR GO; GO:0005739; C:mitochondrion; IDA:HPA.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR InterPro; IPR039626; NOL7.
DR InterPro; IPR012579; NOL7_C.
DR PANTHER; PTHR32337; PTHR32337; 1.
DR Pfam; PF08157; NUC129; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Coiled coil; Isopeptide bond; Nucleus;
KW Phosphoprotein; Reference proteome; Ubl conjugation.
FT CHAIN 1..257
FT /note="Nucleolar protein 7"
FT /id="PRO_0000096937"
FT REGION 1..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 65..104
FT /evidence="ECO:0000255"
FT COMPBIAS 47..65
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..94
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 133
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT CROSSLNK 131
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 162
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 140..145
FT /note="VNLQKK -> GMTILI (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_014791"
FT VAR_SEQ 146..257
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_014792"
FT CONFLICT 200
FT /note="G -> A (in Ref. 5; CAB43398)"
FT /evidence="ECO:0000305"
FT CONFLICT 209
FT /note="N -> D (in Ref. 5; CAB43398)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 257 AA; 29426 MW; 84DB1906362263AF CRC64;
MVQLRPRASR APASAEAMVD EGQLASEEEE AEHGLLLGQP SSGAAAEPLE EDEEGDDEFD
DEAPEELTFA SAQAEAREEE RRVRETVRRD KTLLKEKRKR REELFIEQKK RKLLPDTILE
KLTTASQTNI KKSPGKVKEV NLQKKNEDCE KGNDSKKVKV QKVQSVSQNK SYLAVRLKDQ
DLRDSRQQAA QAFIHNSLYG PGTNRTTVNK FLSLANKRLP VKRAAVQFLN NAWGIQKKQN
AKRFKRRWMV RKMKTKK
