NOL9_ARATH
ID NOL9_ARATH Reviewed; 368 AA.
AC Q8VYP6; Q945L9; Q9LEU0;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Polynucleotide 5'-hydroxyl-kinase NOL9;
DE EC=2.7.1.-;
DE AltName: Full=Nucleolar protein 9 homolog;
GN OrderedLocusNames=At5g11010; ORFNames=T30N20.280;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Polynucleotide 5'-kinase involved in rRNA processing.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q8VYP6; Q39234: TGA3; NbExp=3; IntAct=EBI-21138980, EBI-541366;
CC Q8VYP6; Q8H0Y8: WRKY41; NbExp=3; IntAct=EBI-21138980, EBI-15202502;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VYP6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VYP6-2; Sequence=VSP_040448, VSP_040449;
CC -!- SIMILARITY: Belongs to the Clp1 family. NOL9/GRC3 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB96856.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL365234; CAB96856.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91620.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91621.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91622.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68558.1; -; Genomic_DNA.
DR EMBL; AY070379; AAL49875.1; -; mRNA.
DR EMBL; AY096562; AAM20212.1; -; mRNA.
DR EMBL; AF412075; AAL06528.1; -; mRNA.
DR PIR; T50810; T50810.
DR RefSeq; NP_001318534.1; NM_001343144.1. [Q8VYP6-1]
DR RefSeq; NP_001330301.1; NM_001343145.1. [Q8VYP6-1]
DR RefSeq; NP_568243.1; NM_121139.1. [Q8VYP6-2]
DR RefSeq; NP_850805.1; NM_180474.2. [Q8VYP6-1]
DR AlphaFoldDB; Q8VYP6; -.
DR SMR; Q8VYP6; -.
DR BioGRID; 16246; 3.
DR IntAct; Q8VYP6; 3.
DR STRING; 3702.AT5G11010.2; -.
DR PaxDb; Q8VYP6; -.
DR PRIDE; Q8VYP6; -.
DR ProteomicsDB; 251164; -. [Q8VYP6-1]
DR EnsemblPlants; AT5G11010.1; AT5G11010.1; AT5G11010. [Q8VYP6-2]
DR EnsemblPlants; AT5G11010.2; AT5G11010.2; AT5G11010. [Q8VYP6-1]
DR EnsemblPlants; AT5G11010.3; AT5G11010.3; AT5G11010. [Q8VYP6-1]
DR EnsemblPlants; AT5G11010.4; AT5G11010.4; AT5G11010. [Q8VYP6-1]
DR GeneID; 830968; -.
DR Gramene; AT5G11010.1; AT5G11010.1; AT5G11010. [Q8VYP6-2]
DR Gramene; AT5G11010.2; AT5G11010.2; AT5G11010. [Q8VYP6-1]
DR Gramene; AT5G11010.3; AT5G11010.3; AT5G11010. [Q8VYP6-1]
DR Gramene; AT5G11010.4; AT5G11010.4; AT5G11010. [Q8VYP6-1]
DR KEGG; ath:AT5G11010; -.
DR Araport; AT5G11010; -.
DR TAIR; locus:2183830; AT5G11010.
DR eggNOG; KOG2750; Eukaryota.
DR HOGENOM; CLU_021128_1_0_1; -.
DR OMA; CGPKNAG; -.
DR PhylomeDB; Q8VYP6; -.
DR PRO; PR:Q8VYP6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VYP6; baseline and differential.
DR Genevisible; Q8VYP6; AT.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0051731; F:polynucleotide 5'-hydroxyl-kinase activity; ISS:UniProtKB.
DR GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IBA:GO_Central.
DR GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR045116; Clp1/Grc3.
DR InterPro; IPR032319; CLP1_P.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12755; PTHR12755; 1.
DR Pfam; PF16575; CLP1_P; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Kinase; Nucleotide-binding; Nucleus;
KW Reference proteome; rRNA processing; Transferase.
FT CHAIN 1..368
FT /note="Polynucleotide 5'-hydroxyl-kinase NOL9"
FT /id="PRO_0000403781"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 310..318
FT /note="IVRGIDTER -> KFLFIHCNQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_040448"
FT VAR_SEQ 319..368
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_040449"
SQ SEQUENCE 368 AA; 41481 MW; 4688B336A0B4FEE0 CRC64;
MSEAENKLPF IPEEWSNAAS SVSCSSLQPV IALVCGPKNS GKSTFSRNLV EVLLQRYKRV
AYLDTDVGQP EFTAPGFLSL TIVDKSILES DWTVPCVKTP ERCFFYGDVS SKRDPKAYLR
YVYTLFDYYQ LHFCKSSENK TELPLVINTP GWVKGIGYEL LVDVLRYVSP SHVVKINISA
YNKNLPAGLF WLDGNDDETA HLIEIQSAYQ DRYNQSILIH KDARLMRDMR IIAYFRQCFK
GKEVNTIKEL THELASHIPY EVPISSLTIN HLHCQIPSSE VYYSLNASIV GLGISTEVFE
DLPSCVGLGI VRGIDTERGI LYVITPVPEN LVEKVDLLLQ GYIQLPTCLL EVKDYRSPYL
SANVLAST
