ID   NOL9_BOVIN              Reviewed;         694 AA.
AC   E1BPN0;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Polynucleotide 5'-hydroxyl-kinase NOL9;
DE            EC=2.7.1.-;
DE   AltName: Full=Nucleolar protein 9;
GN   Name=NOL9;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=19390049; DOI=10.1126/science.1169588;
RG   The bovine genome sequencing and analysis consortium;
RT   "The genome sequence of taurine cattle: a window to ruminant biology and
RT   evolution.";
RL   Science 324:522-528(2009).
CC   -!- FUNCTION: Polynucleotide 5'-kinase involved in rRNA processing. The
CC       kinase activity is required for the processing of the 32S precursor
CC       into 5.8S and 28S rRNAs, more specifically for the generation of the
CC       major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase
CC       activities. Probably binds RNA (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with PELP1, WDR18 and SENP3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Nucleus, nucleolus
CC       {ECO:0000250}. Note=Colocalizes with pre-60S rRNP particles.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Clp1 family. NOL9/GRC3 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AAFC03030715; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001193528.1; NM_001206599.1.
DR   AlphaFoldDB; E1BPN0; -.
DR   SMR; E1BPN0; -.
DR   STRING; 9913.ENSBTAP00000022240; -.
DR   PaxDb; E1BPN0; -.
DR   PRIDE; E1BPN0; -.
DR   GeneID; 523474; -.
DR   KEGG; bta:523474; -.
DR   CTD; 79707; -.
DR   eggNOG; KOG2750; Eukaryota.
DR   HOGENOM; CLU_021128_2_0_1; -.
DR   InParanoid; E1BPN0; -.
DR   OrthoDB; 1217334at2759; -.
DR   TreeFam; TF313802; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0051731; F:polynucleotide 5'-hydroxyl-kinase activity; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR   GO; GO:0000460; P:maturation of 5.8S rRNA; ISS:UniProtKB.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR045116; Clp1/Grc3.
DR   InterPro; IPR032319; CLP1_P.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12755; PTHR12755; 1.
DR   Pfam; PF16575; CLP1_P; 1.
PE   3: Inferred from homology;
KW   Acetylation; ATP-binding; Isopeptide bond; Kinase; Nucleotide-binding;
KW   Nucleus; Reference proteome; RNA-binding; rRNA processing; Transferase;
KW   Ubl conjugation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q5SY16"
FT   CHAIN           2..694
FT                   /note="Polynucleotide 5'-hydroxyl-kinase NOL9"
FT                   /id="PRO_0000403777"
FT   REGION          80..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         298..305
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5SY16"
FT   CROSSLNK        477
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0000250|UniProtKB:Q5SY16"
SQ   SEQUENCE   694 AA;  78133 MW;  4EE2044EE5B9D99A CRC64;
     MADSLLLLKR VPSRHTWLRA RKARPQLMLS RRPRRRLRNL RWRSRRRLRR RLLQAQAAGA
     DWLQGGCLVS GDAALQRLKT SARRRASSPE PAEDPVPSGP AILPIPPVRP AGSGRAVLLL
     PLGQGFTFSG ICCVTCLYGQ VQVFGFTISQ GQPAQNVFST YTHSRLTINA VHYSVHEKSK
     KEMKREARVL LRPYLNQDDR YCLMSSFSPL CSIVLLERLK TSTVNFIISH PGLSYIFVQE
     VRTFQINSEY FALRSVGIRR EKKKTGLRLT ESAFAVMEEL VSISSEEADS CPVILVCGCQ
     DIGKSTFNRY LINQLLNSVS CVDYLECDLG QTEFTPPGCI SLLNITEPVL GPPFTHQRTP
     QKMVYYGKTS CKNNFENYIE VIKYVFSSYK RESPLIINTM GWVADQGLLL LIDLIRLLSP
     SHVVQFSSDR SKYMPDLTPD FVDDMDGLYT RRRSRVRNRG FHLPEFAESL EFADEEKEGP
     VMSTGYKLMF VKSEFVTGKT SRNRESHNRI LRELAVLGYL SQLQPPVPKP LRALHGLTPY
     QVPFNAVALR IIHADVAPTH ILYAVNASWV GLCKILDDVR GYASGPILLA QSPICDCVGF
     GICRGIDMEK KLYHILTPVP PEELRHVNCL LVGAVNIPQC VLKSQGGLEG TIPYVTTDYN
     FKLPGASEKI GARESGATYK EKGHPKPKFY RKTY