NOLC1_CAEEL
ID NOLC1_CAEEL Reviewed; 971 AA.
AC Q9XVS4; Q564W7;
DT 12-SEP-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Nucleolar protein dao-5 {ECO:0000305};
DE AltName: Full=140 kDa nucleolar phosphoprotein {ECO:0000250|UniProtKB:Q14978};
DE Short=Nopp140 {ECO:0000250|UniProtKB:Q14978};
DE AltName: Full=Dauer or aging adult overexpression protein 5 {ECO:0000312|WormBase:C25A1.10a};
GN Name=dao-5 {ECO:0000303|PubMed:11743719, ECO:0000312|WormBase:C25A1.10a};
GN ORFNames=C25A1.10 {ECO:0000312|WormBase:C25A1.10a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=11743719; DOI=10.1006/jmbi.2000.5210;
RA Yu H., Larsen P.L.;
RT "DAF-16-dependent and independent expression targets of DAF-2 insulin
RT receptor-like pathway in Caenorhabditis elegans include FKBPs.";
RL J. Mol. Biol. 314:1017-1028(2001).
RN [3] {ECO:0000305}
RP SUBUNIT, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=20405020; DOI=10.1371/journal.pone.0010161;
RA Hadwiger G., Dour S., Arur S., Fox P., Nonet M.L.;
RT "A monoclonal antibody toolkit for C. elegans.";
RL PLoS ONE 5:E10161-E10161(2010).
RN [4] {ECO:0000305}
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=22768349; DOI=10.1371/journal.pone.0040290;
RA Korcekova D., Gombitova A., Raska I., Cmarko D., Lanctot C.;
RT "Nucleologenesis in the Caenorhabditis elegans embryo.";
RL PLoS ONE 7:E40290-E40290(2012).
RN [5] {ECO:0000305}
RP FUNCTION, INTERACTION WITH RNA POLYMERASE I, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=24722283; DOI=10.1038/cddis.2014.114;
RA Lee C.C., Tsai Y.T., Kao C.W., Lee L.W., Lai H.J., Ma T.H., Chang Y.S.,
RA Yeh N.H., Lo S.J.;
RT "Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases
RT germ cell apoptosis in C. elegans.";
RL Cell Death Dis. 5:E1158-E1158(2014).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=27003693; DOI=10.1080/19491034.2016.1166322;
RA Ma T.H., Lee L.W., Lee C.C., Yi Y.H., Chan S.P., Tan B.C., Lo S.J.;
RT "Genetic control of nucleolar size: An evolutionary perspective.";
RL Nucleus 7:112-120(2016).
CC -!- FUNCTION: Nucleolar protein which binds to RNA polymerase I and rDNA
CC and is required for efficient RNA polymerase I-mediated rDNA
CC transcription (PubMed:24722283). Maintains the epigenetically active
CC status of rDNA chromatin which facilitates rDNA transcription and
CC sustains germline development, ensuring fertility (PubMed:24722283).
CC Plays a role in the modulation of nucleolus size (PubMed:27003693). May
CC play a role in the regulation of lifespan (PubMed:11743719).
CC {ECO:0000269|PubMed:24722283, ECO:0000269|PubMed:27003693,
CC ECO:0000305|PubMed:11743719}.
CC -!- SUBUNIT: May form dimers (PubMed:20405020). Interacts with RNA
CC polymerase I (PubMed:24722283). {ECO:0000269|PubMed:20405020,
CC ECO:0000269|PubMed:24722283}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:20405020,
CC ECO:0000269|PubMed:22768349, ECO:0000269|PubMed:24722283}. Nucleus,
CC nucleoplasm {ECO:0000269|PubMed:20405020, ECO:0000269|PubMed:22768349}.
CC Note=Prior to fertilization, during the diakinesis stage of meiotic
CC prophase, transcription ceases and mature oocytes harbor no nucleolus.
CC During this time, localizes to nucleoli of transcriptionally active
CC cells in the rest of the gonad. From the 2-cell stage of embryonic
CC development, localizes to the nucleoplasm. Localizes to nucleoli upon
CC their development in 6- to 8-cell stage embryos. Localizes to the
CC nuclear periphery in developing embryos. In postembryonic cells,
CC predominantly localizes to the nucleolus, but also localizes to small
CC punctate structures throughout the nucleoplasm. Co-localizes with rDNA
CC and fib-1 in nucleoli. {ECO:0000269|PubMed:22768349}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:C25A1.10a};
CC IsoId=Q9XVS4-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:C25A1.10b};
CC IsoId=Q9XVS4-2; Sequence=VSP_059730;
CC -!- TISSUE SPECIFICITY: Expressed in the nerve ring and hypodermal tissues
CC (PubMed:24722283). Expressed in the intestine (PubMed:20405020,
CC PubMed:24722283). Expressed in the germline (PubMed:20405020).
CC {ECO:0000269|PubMed:20405020, ECO:0000269|PubMed:24722283}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout embryonic development and in
CC adults (PubMed:22768349). During embryogenesis, expressed from the 2-
CC cell stage and persists throughout development in transcriptionally
CC active cells (PubMed:22768349). Expression is higher in dauer larvae
CC than in adults (PubMed:11743719). {ECO:0000269|PubMed:11743719,
CC ECO:0000269|PubMed:22768349}.
CC -!- SIMILARITY: Belongs to the NOLC1 family. {ECO:0000305}.
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DR EMBL; BX284601; CAB02755.1; -; Genomic_DNA.
DR EMBL; BX284601; CAI79153.1; -; Genomic_DNA.
DR PIR; T19431; T19431.
DR RefSeq; NP_001021004.1; NM_001025833.6. [Q9XVS4-1]
DR RefSeq; NP_001021005.1; NM_001025834.4. [Q9XVS4-2]
DR AlphaFoldDB; Q9XVS4; -.
DR STRING; 6239.C25A1.10a; -.
DR EPD; Q9XVS4; -.
DR PaxDb; Q9XVS4; -.
DR PeptideAtlas; Q9XVS4; -.
DR EnsemblMetazoa; C25A1.10a.1; C25A1.10a.1; WBGene00000931. [Q9XVS4-1]
DR EnsemblMetazoa; C25A1.10b.1; C25A1.10b.1; WBGene00000931. [Q9XVS4-2]
DR GeneID; 266845; -.
DR KEGG; cel:CELE_C25A1.10; -.
DR UCSC; C25A1.10a; c. elegans.
DR CTD; 266845; -.
DR WormBase; C25A1.10a; CE08376; WBGene00000931; dao-5. [Q9XVS4-1]
DR WormBase; C25A1.10b; CE38279; WBGene00000931; dao-5. [Q9XVS4-2]
DR eggNOG; KOG2992; Eukaryota.
DR GeneTree; ENSGT00730000111092; -.
DR HOGENOM; CLU_299803_0_0_1; -.
DR InParanoid; Q9XVS4; -.
DR OMA; GGPINQS; -.
DR OrthoDB; 1580584at2759; -.
DR PRO; PR:Q9XVS4; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00000931; Expressed in embryo and 4 other tissues.
DR GO; GO:0005730; C:nucleolus; IDA:WormBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:WormBase.
DR GO; GO:0000182; F:rDNA binding; IDA:UniProtKB.
DR GO; GO:0001042; F:RNA polymerase I core binding; IDA:WormBase.
DR GO; GO:0040024; P:dauer larval development; TAS:WormBase.
DR GO; GO:0008340; P:determination of adult lifespan; IGI:WormBase.
DR GO; GO:0008286; P:insulin receptor signaling pathway; IGI:WormBase.
DR GO; GO:0042790; P:nucleolar large rRNA transcription by RNA polymerase I; IMP:UniProtKB.
DR GO; GO:0061187; P:regulation of ribosomal DNA heterochromatin assembly; IMP:UniProtKB.
DR GO; GO:0009303; P:rRNA transcription; IMP:WormBase.
DR InterPro; IPR007718; Srp40_C.
DR Pfam; PF05022; SRP40_C; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome.
FT CHAIN 1..971
FT /note="Nucleolar protein dao-5"
FT /evidence="ECO:0000305"
FT /id="PRO_0000445021"
FT REGION 61..926
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 938..971
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..91
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..197
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..265
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..296
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 310..325
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..356
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 372..387
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 403..418
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 434..449
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 463..477
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 525..540
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 556..571
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 585..599
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 603..617
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 647..661
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 677..693
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 734..753
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 763..781
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 796..815
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 830..846
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 886..924
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 955..971
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 247..521
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_059730"
SQ SEQUENCE 971 AA; 101098 MW; 832936115B58709B CRC64;
MSSDLYANAV LYSEISQRNP ELVSKMFNEA TRKKLDAFLK DNEAPKLKDI VTDNVFKKRK
RIASSSGNEP PVKKMAAKDS SDSDSSDDGA VKKNGVQKPV NVAAKKSATP VQKKAESSSS
SDSDAPAKKL TPVKKPAQTP AQKKAASSSD SDSDDEPPKK APAVTTKVAP KPMAKKQDTS
DSDSDSEDSD DGKSKKANPV KVTPVANVLQ KVVAKKAASS SSDSSDDEKK PAAKPTPAKP
TPKPVVKKAE SSSDSSDDEK KPVAKPAPAK ATPKPAAKKA DSSSDSSDDE APAKKTPAKA
APKPVAKKAE SSSDSSDDEK KPAAKPTPAK ATPKPVAKKA ESSSDSSDDE KKPVAKPAPA
KATPKPVAKK AESSSDSSDD EKKPAAKPTP AKATPKPVAK KAESSSDSSD DEKKPVAKPT
SAKATPKPAA KKADSSSDSS DDEAPAKKTP AKAAPKPASK KAESSSDSSD DEKPAAKSTP
AKITPKPTAK KVASSSSDSS DDEKKPAAKP TPANATPKPV AKKAESSSDS SDDEKKPVAK
PTSAKATPKP AAKKADLSSD FSDDEAPAKK TPAKAAPKPA SKKAESSSDS SDDEKPAAKS
TPAKTTPKPT AKKAASSSSD SSDDEKKPVA KPTSAKATPK PAAKKADSSS DSSDDEAPAK
KTPVKPTPVK IVAKKVDSSS DSSDDEKKPT KATPVKVTPK SVTKKAAASS SDSSDDEKKP
VVKQTPNVVP KKEKAASSSD DSSDDEKKPT AKPTPKATPK QSAKKADSSD DSSDDEAPAK
KTPAKSTPAK TAVKKEASSS SDDSSDDEKT KKKSATTPAK STPKTALKKA ESSDSSDDDE
DLPKPSKAVT PRPQRADSEE SAETEESSSR TPALKAKPLA TSTEKAVYEN RKRKSSPFRR
VQMTKDSVSE KFRNNQHDSH FDQWGQRANE SLGKVVGKAF RHEKTKKKKG SYGGGPINQS
INSIKFSDSD D
