ID   NOLT_RHIFR              Reviewed;         289 AA.
AC   P33209;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Nodulation protein NolT;
DE   Flags: Precursor;
GN   Name=nolT;
OS   Rhizobium fredii (Sinorhizobium fredii).
OG   Plasmid sym.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=380;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 257;
RX   PubMed=8412662; DOI=10.1111/j.1365-2958.1993.tb01665.x;
RA   Meinhardt L.W., Krishnan H.B., Balatti P.A., Pueppke S.G.;
RT   "Molecular cloning and characterization of a sym plasmid locus that
RT   regulates cultivar-specific nodulation of soybean by Rhizobium fredii
RT   USDA257.";
RL   Mol. Microbiol. 9:17-29(1993).
CC   -!- FUNCTION: Regulates cultivar-specific nodulation of soybean.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- INDUCTION: By flavonoid signal compounds.
CC   -!- SIMILARITY: Belongs to the YscJ lipoprotein family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L12251; AAB17677.1; -; Genomic_DNA.
DR   PIR; S35022; S35022.
DR   AlphaFoldDB; P33209; -.
DR   SMR; P33209; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   Gene3D; 3.30.300.30; -; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR006182; FliF_N_dom.
DR   InterPro; IPR003282; T3SS_HrcJ/YscJ.
DR   InterPro; IPR043427; YscJ/FliF.
DR   PANTHER; PTHR30046; PTHR30046; 1.
DR   Pfam; PF01514; YscJ_FliF; 1.
DR   PRINTS; PR01338; TYPE3OMKPROT.
DR   TIGRFAMs; TIGR02544; III_secr_YscJ; 1.
PE   2: Evidence at transcript level;
KW   Cell outer membrane; Lipoprotein; Membrane; Nodulation; Palmitate; Plasmid;
KW   Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000305"
FT   CHAIN           34..289
FT                   /note="Nodulation protein NolT"
FT                   /id="PRO_0000018227"
FT   TRANSMEM        233..253
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   LIPID           34
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
FT   LIPID           34
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   289 AA;  31241 MW;  5944DE5C9A60DB2B CRC64;
     MFGSAHGDTT SSDTSGRRPL RLVVLPLLLA LSSCKVDLYT QLQEREANEI VALLMDNGVD
     AVRVAGKDGT STIQVDEKLL AFSIKLLNGK GLPRQSFKNL GEIFQGSGLI ASPTEERARY
     VYALSEELSH TISDIDGVFS ARVHVVLPHN DLLRAGDTPS SASVFIRHDA KTNLPALLPK
     IKMLVAESIE GLAYDKVEVV LVPVERSAQE QRSLLEPDLA QASRPIPVPL LAVAVGVGAA
     VFAVTCYLLF IVLGHRRRQL TGELSRVQER PGVSALAAIR KKIPALGRR