ID   NOLT_SINFN              Reviewed;         289 AA.
AC   P55714;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 120.
DE   RecName: Full=Nodulation protein NolT;
DE   Flags: Precursor;
GN   Name=nolT; OrderedLocusNames=NGR_a00670; ORFNames=y4yF;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the YscJ lipoprotein family. {ECO:0000305}.
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DR   EMBL; U00090; AAB91945.1; -; Genomic_DNA.
DR   RefSeq; NP_444158.1; NC_000914.2.
DR   RefSeq; WP_010875108.1; NC_000914.2.
DR   AlphaFoldDB; P55714; -.
DR   SMR; P55714; -.
DR   STRING; 394.NGR_a00670; -.
DR   EnsemblBacteria; AAB91945; AAB91945; NGR_a00670.
DR   KEGG; rhi:NGR_a00670; -.
DR   eggNOG; COG4669; Bacteria.
DR   HOGENOM; CLU_073268_0_0_5; -.
DR   OMA; GRIQQMV; -.
DR   OrthoDB; 858412at2; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   Gene3D; 3.30.300.30; -; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR006182; FliF_N_dom.
DR   InterPro; IPR003282; T3SS_HrcJ/YscJ.
DR   InterPro; IPR043427; YscJ/FliF.
DR   PANTHER; PTHR30046; PTHR30046; 1.
DR   Pfam; PF01514; YscJ_FliF; 1.
DR   PRINTS; PR01338; TYPE3OMKPROT.
DR   TIGRFAMs; TIGR02544; III_secr_YscJ; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Nodulation; Palmitate; Plasmid;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000305"
FT   CHAIN           34..289
FT                   /note="Nodulation protein NolT"
FT                   /id="PRO_0000018228"
FT   TRANSMEM        233..253
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   LIPID           34
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
FT   LIPID           34
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   289 AA;  31252 MW;  4007B213F663230F CRC64;
     MFGSAHGDTT SSDTSGRRPL RLVVLPLLLA LSSCKVDLYT QLQEREANEM LALLMDSGVD
     AVRVAGKDGT STIQVDEKLL AFSIKLLNAK GLPRQSFKNL GEIFQGSGLI ASPTEERARY
     VYALSEELSH TISDIDGVFS ARVHVVLPHN DLLRAGDTPS SASVFIRHDA KTNLPALLPK
     IKMLVAESIE GLAYDKVEVV LVPVERSAQE QRSLLATDLA QASRPIPEPL LAVAVGVSAA
     VFAVTCYLLF IVLGHRRRQL TGELSRVQER PGVSALAAIR KKIPGLGRR