NOL_ORYSJ
ID NOL_ORYSJ Reviewed; 343 AA.
AC Q84ST4; A0A0P0W0V8; Q10FV1; Q6AT49;
DT 09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Chlorophyll(ide) b reductase NOL, chloroplastic;
DE EC=1.1.1.294;
DE AltName: Full=Protein NON-YELLOW COLORING 1-LIKE;
DE Short=OsNOL;
DE Short=Protein NYC1-LIKE;
DE AltName: Full=Short-chain dehydrogenase/reductase NOL;
DE Flags: Precursor;
GN Name=NOL; OrderedLocusNames=Os03g0654600, LOC_Os03g45194;
GN ORFNames=OsJ_11943, OSJNBa0092N01.25, OSJNBb0023J24.13;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=17416733; DOI=10.1105/tpc.106.042911;
RA Kusaba M., Ito H., Morita R., Iida S., Sato Y., Fujimoto M., Kawasaki S.,
RA Tanaka R., Hirochika H., Nishimura M., Tanaka A.;
RT "Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and
RT grana degradation during leaf senescence.";
RL Plant Cell 19:1362-1375(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP INTERACTION WITH NCY1, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18778405; DOI=10.1111/j.1365-313x.2008.03670.x;
RA Sato Y., Morita R., Katsuma S., Nishimura M., Tanaka A., Kusaba M.;
RT "Two short-chain dehydrogenase/reductases, NON-YELLOW COLORING 1 and NYC1-
RT LIKE, are required for chlorophyll b and light-harvesting complex II
RT degradation during senescence in rice.";
RL Plant J. 57:120-131(2009).
CC -!- FUNCTION: Required for chlorophyll b degradation.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7(1)-hydroxychlorophyllide a + NAD(+) = chlorophyllide b +
CC H(+) + NADH; Xref=Rhea:RHEA:24768, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:83356,
CC ChEBI:CHEBI:83357; EC=1.1.1.294;
CC Evidence={ECO:0000269|PubMed:17416733};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7(1)-hydroxychlorophyllide a + NADP(+) = chlorophyllide b +
CC H(+) + NADPH; Xref=Rhea:RHEA:24772, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:83356,
CC ChEBI:CHEBI:83357; EC=1.1.1.294;
CC Evidence={ECO:0000269|PubMed:17416733};
CC -!- SUBUNIT: Interacts with NCY1 to form a complex that acts as a
CC chlorophyll b reductase. {ECO:0000269|PubMed:18778405}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:18778405}; Peripheral membrane protein
CC {ECO:0000269|PubMed:18778405}; Stromal side
CC {ECO:0000269|PubMed:18778405}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves and stems. Also detected in
CC non-photosynthetic tissues such as roots.
CC {ECO:0000269|PubMed:17416733}.
CC -!- DEVELOPMENTAL STAGE: Strongly expressed in late-senescing leaves.
CC {ECO:0000269|PubMed:17416733}.
CC -!- INDUCTION: Up-regulated by dark treatment.
CC {ECO:0000269|PubMed:18778405}.
CC -!- DISRUPTION PHENOTYPE: Stay-green phenotype during senescence. Grana
CC stacks fused into large stable stacks during semescence.
CC {ECO:0000269|PubMed:18778405}.
CC -!- MISCELLANEOUS: Chlorophyll b reductase activity detected in vitro with
CC a recombinant protein produced in a heterologous system.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT78834.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=ABF97956.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB255026; BAF49741.1; -; mRNA.
DR EMBL; AC120535; AAO73232.1; -; Genomic_DNA.
DR EMBL; AC138004; AAT78833.1; -; Genomic_DNA.
DR EMBL; AC138004; AAT78834.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000009; ABF97956.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; BAS85540.1; -; Genomic_DNA.
DR EMBL; CM000140; EEE59614.1; -; Genomic_DNA.
DR RefSeq; XP_015628274.1; XM_015772788.1.
DR AlphaFoldDB; Q84ST4; -.
DR SMR; Q84ST4; -.
DR STRING; 4530.OS03T0654600-01; -.
DR PRIDE; Q84ST4; -.
DR EnsemblPlants; Os03t0654600-01; Os03t0654600-01; Os03g0654600.
DR GeneID; 4333604; -.
DR Gramene; Os03t0654600-01; Os03t0654600-01; Os03g0654600.
DR KEGG; osa:4333604; -.
DR eggNOG; KOG0725; Eukaryota.
DR HOGENOM; CLU_010194_2_4_1; -.
DR InParanoid; Q84ST4; -.
DR OMA; HIDLWIN; -.
DR OrthoDB; 1190834at2759; -.
DR BRENDA; 1.1.1.294; 4460.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q84ST4; OS.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0034256; F:chlorophyll(ide) b reductase activity; IDA:UniProtKB.
DR GO; GO:0015996; P:chlorophyll catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 1: Evidence at protein level;
KW Chlorophyll catabolism; Chloroplast; Membrane; NAD; Oxidoreductase;
KW Plastid; Reference proteome; Thylakoid; Transit peptide.
FT TRANSIT 1..54
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 55..343
FT /note="Chlorophyll(ide) b reductase NOL, chloroplastic"
FT /id="PRO_0000391414"
FT ACT_SITE 228
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 78..102
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 343 AA; 37004 MW; 5A0A05B89ABC6E3D CRC64;
MAATAAYLPL RAQAQVGLAP LRPSGSAAAG ARLPGRTARR RLAARGGPEA AGIRAEAVPG
GGGVARRAAM VPPYNVLITG STKGIGYALA KEFLKAGDNV VICSRSAERV ESAVTDLKKE
FGEQHVWGIV CDVREGKDVK ALVDFARDKM KYIDIWINNA GSNAYSYKPL VETSDEALME
VITTNTLGLM ICCREAINMM RNQPRGGHIF NIDGAGSDGR PTPRFAAYGA TKRSVVHLTK
SLQAELQMNE VNNVMVHNLS PGMVTTDLLM SGATTKQAKF FINILAEPAN VVADYLVPNI
RAIPTNQSMK PTYIRFLTGL KAYSRIFSRI AFGARRNKYV AED
