ID   NOM1_MOUSE              Reviewed;         854 AA.
AC   Q3UFM5; E9QPB9;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Nucleolar MIF4G domain-containing protein 1;
DE   AltName: Full=SGD1 homolog;
GN   Name=Nom1; Synonyms=Gm1040;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314 AND SER-315, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Lung, and Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Plays a role in targeting PPP1CA to the nucleolus.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: May interact with EIF4A1, EIF4A2 and EIF4A3. Interacts with
CC       PPP1CA and PPP1CC (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CWC22 family. {ECO:0000305}.
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DR   EMBL; AK148406; BAE28535.1; -; mRNA.
DR   EMBL; AC171500; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS19149.1; -.
DR   RefSeq; NP_001028629.2; NM_001033457.2.
DR   AlphaFoldDB; Q3UFM5; -.
DR   SMR; Q3UFM5; -.
DR   BioGRID; 241474; 1.
DR   STRING; 10090.ENSMUSP00000001611; -.
DR   iPTMnet; Q3UFM5; -.
DR   PhosphoSitePlus; Q3UFM5; -.
DR   EPD; Q3UFM5; -.
DR   MaxQB; Q3UFM5; -.
DR   PaxDb; Q3UFM5; -.
DR   PeptideAtlas; Q3UFM5; -.
DR   PRIDE; Q3UFM5; -.
DR   ProteomicsDB; 293700; -.
DR   Antibodypedia; 56748; 68 antibodies from 19 providers.
DR   Ensembl; ENSMUST00000001611; ENSMUSP00000001611; ENSMUSG00000001569.
DR   GeneID; 433864; -.
DR   KEGG; mmu:433864; -.
DR   UCSC; uc008wuj.1; mouse.
DR   CTD; 64434; -.
DR   MGI; MGI:1861749; Nom1.
DR   VEuPathDB; HostDB:ENSMUSG00000001569; -.
DR   eggNOG; KOG2141; Eukaryota.
DR   GeneTree; ENSGT00940000153458; -.
DR   HOGENOM; CLU_006786_0_1_1; -.
DR   InParanoid; Q3UFM5; -.
DR   OMA; QFKQSIR; -.
DR   OrthoDB; 307186at2759; -.
DR   PhylomeDB; Q3UFM5; -.
DR   TreeFam; TF312808; -.
DR   BioGRID-ORCS; 433864; 23 hits in 76 CRISPR screens.
DR   ChiTaRS; Nom1; mouse.
DR   PRO; PR:Q3UFM5; -.
DR   Proteomes; UP000000589; Chromosome 5.
DR   RNAct; Q3UFM5; protein.
DR   Bgee; ENSMUSG00000001569; Expressed in spermatocyte and 77 other tissues.
DR   GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0048820; P:hair follicle maturation; IMP:MGI.
DR   GO; GO:0042274; P:ribosomal small subunit biogenesis; IBA:GO_Central.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR   InterPro; IPR003890; MIF4G-like_typ-3.
DR   Pfam; PF02847; MA3; 1.
DR   Pfam; PF02854; MIF4G; 1.
DR   SMART; SM00544; MA3; 1.
DR   SMART; SM00543; MIF4G; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51366; MI; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..854
FT                   /note="Nucleolar MIF4G domain-containing protein 1"
FT                   /id="PRO_0000286824"
FT   DOMAIN          356..553
FT                   /note="MIF4G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT   DOMAIN          648..764
FT                   /note="MI"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT   REGION          2..275
FT                   /note="Necessary for nucleolar localization and for
FT                   targeting PPP1CA to the nucleolus"
FT                   /evidence="ECO:0000250"
FT   REGION          66..215
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          231..333
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           301..304
FT                   /note="Required for efficient binding to PPP1CA and for
FT                   targeting PPP1CA to the nucleolus"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        75..98
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        140..154
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        179..208
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        277..333
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         60
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5C9Z4"
FT   MOD_RES         311
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5C9Z4"
FT   MOD_RES         314
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         315
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   CONFLICT        309
FT                   /note="E -> D (in Ref. 1; BAE28535)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   854 AA;  95960 MW;  0A9BC99B9F521475 CRC64;
     MPRNVPEVNG VYRHGACELW CRAMAHALLK RSGVRRGLGG REGPLKRLRL AVEDFVRTTS
     ESEACESRSA VARSRPGGRK SRKELRKEKR HLRKARRLQR TVGSGSGDQG GNVGLNDGPE
     TRRPPTEVRP TPAKATATPA KASAPSTNTK ASAAQPKAKA KGAPGKPGPA TATARKRALL
     AANEEEDREI RKLERCLGLH KRKKKGDGSS VPLSFARDGL DYILGALECG SGGGLYESSE
     EEEEEKLETG QTVLESDLES NSKESEEDPD WQVLQEDQED VNSKRRGEAE SGTRGNKGTK
     KVRFAEVVEK SRSSSEDDIE QQESHSVESG EKYIPPRLRN EEVIDVHKKE ELDRLKKHVK
     GLINRLSEPN MASISGQLEE LYMAHSRKDM NDTLTTALMD ACVTASAMPS RLMMEHVFLV
     SILHHTVGIE VGACFLEAVV KKFDAIYRDG GEGKELDNLF TMIAHLYNFH VVQSILIFDI
     LKKLVGTFTE KDIELILLML KNVGFALRKD DALSLKELIT EAQTQASGAG NKFQDQNRVR
     FMLETMLALK NNDLRKIPGY NPEPVEKLKK LQRTLVRNAG SGSETRLRIS WDGILNAEQT
     GRWWIVGSAW SGTPMIDNSH HIQLQKPLAG MASSKMLELA RKQRMNTDVR RIIFCTLMTS
     EDFLDAFEKL LKLGLKDQQE REIVHILMDC CLQEKTYNPF YAFLASKFCD YERRFQMTFQ
     FSIWDKFRDL ENLPDTKFSN LVHLLAHLLR TKSLPLSVLK VVEFSELDKP RVHFLRRVLT
     ALLMETEDDD LAVIFSRVSD NPKLGMLREG LKLFIGHFLL KHTQAHQSAE EASLLREKAG
     LASKSLQGKA ILRM