NONA_DROME
ID NONA_DROME Reviewed; 700 AA.
AC Q04047; Q24261; Q59E42; Q9VXH1;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Protein no-on-transient A;
DE AltName: Full=Puff-specific protein Bj6;
GN Name=nonA; ORFNames=CG4211;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND FUNCTION.
RX PubMed=2344408; DOI=10.1016/0896-6273(90)90197-n;
RA Jones K.R., Rubin G.M.;
RT "Molecular analysis of no-on-transient A, a gene required for normal vision
RT in Drosophila.";
RL Neuron 4:711-723(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R;
RX PubMed=2129286; DOI=10.1007/bf00337601;
RA Von Besser H., Schnabel P., Wieland C., Fritz E., Stanewsky R.,
RA Saumweber H.;
RT "The puff-specific Drosophila protein Bj6, encoded by the gene no-on
RT transient A, shows homology to RNA-binding proteins.";
RL Chromosoma 100:37-47(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Required for normal vision and courtship behavior in
CC Drosophila. {ECO:0000269|PubMed:2344408}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=A, I;
CC IsoId=Q04047-1; Sequence=Displayed;
CC Name=2; Synonyms=C, II;
CC IsoId=Q04047-2; Sequence=VSP_005801;
CC -!- DEVELOPMENTAL STAGE: Expressed ubiquitously during embryonic
CC development with prominent expression during the first 12 hours of
CC embryogenesis. {ECO:0000269|PubMed:2129286}.
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DR EMBL; M33496; AAA03214.1; -; Unassigned_DNA.
DR EMBL; M33496; AAA03215.1; -; Unassigned_DNA.
DR EMBL; X55902; CAA39395.1; -; mRNA.
DR EMBL; AE014298; AAF48597.2; -; Genomic_DNA.
DR EMBL; AE014298; AAX52501.1; -; Genomic_DNA.
DR EMBL; AY119651; AAM50305.1; -; mRNA.
DR PIR; JH0162; JH0162.
DR PIR; JH0163; JH0163.
DR RefSeq; NP_001014747.1; NM_001014747.2. [Q04047-2]
DR RefSeq; NP_001285324.1; NM_001298395.1. [Q04047-1]
DR RefSeq; NP_523367.2; NM_078643.3. [Q04047-1]
DR AlphaFoldDB; Q04047; -.
DR SMR; Q04047; -.
DR BioGRID; 58943; 20.
DR IntAct; Q04047; 6.
DR MINT; Q04047; -.
DR STRING; 7227.FBpp0074012; -.
DR iPTMnet; Q04047; -.
DR PaxDb; Q04047; -.
DR PRIDE; Q04047; -.
DR ABCD; Q04047; 4 sequenced antibodies.
DR DNASU; 32603; -.
DR EnsemblMetazoa; FBtr0074232; FBpp0074011; FBgn0004227. [Q04047-1]
DR EnsemblMetazoa; FBtr0100586; FBpp0100043; FBgn0004227. [Q04047-2]
DR EnsemblMetazoa; FBtr0340313; FBpp0309274; FBgn0004227. [Q04047-1]
DR GeneID; 32603; -.
DR KEGG; dme:Dmel_CG4211; -.
DR CTD; 32603; -.
DR FlyBase; FBgn0004227; nonA.
DR VEuPathDB; VectorBase:FBgn0004227; -.
DR eggNOG; KOG0115; Eukaryota.
DR GeneTree; ENSGT00940000169018; -.
DR HOGENOM; CLU_027185_3_0_1; -.
DR InParanoid; Q04047; -.
DR OMA; KHGPQKQ; -.
DR PhylomeDB; Q04047; -.
DR SignaLink; Q04047; -.
DR BioGRID-ORCS; 32603; 1 hit in 3 CRISPR screens.
DR ChiTaRS; nonA; fly.
DR GenomeRNAi; 32603; -.
DR PRO; PR:Q04047; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0004227; Expressed in eye disc (Drosophila) and 22 other tissues.
DR ExpressionAtlas; Q04047; baseline and differential.
DR Genevisible; Q04047; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0045433; P:male courtship behavior, veined wing generated song production; IMP:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IC:FlyBase.
DR GO; GO:0042331; P:phototaxis; NAS:FlyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0007632; P:visual behavior; NAS:FlyBase.
DR GO; GO:0007601; P:visual perception; IMP:FlyBase.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012975; NOPS.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF08075; NOPS; 1.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome;
KW Repeat; RNA-binding; Sensory transduction; Vision.
FT CHAIN 1..700
FT /note="Protein no-on-transient A"
FT /id="PRO_0000081662"
FT DOMAIN 302..374
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 376..457
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 568..592
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 677..700
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 505..616
FT /evidence="ECO:0000255"
FT COMPBIAS 1..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..133
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 142..235
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 236
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 666..700
FT /note="DSFAFEFGVNNMNQGGNQRGNNGGGNNVPWGRRRF -> VCPHPKYYPTKYS
FT VTNRSVELQELLSMIPFMKL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:2344408"
FT /id="VSP_005801"
FT CONFLICT 118
FT /note="P -> S (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 350
FT /note="A -> R (in Ref. 2; CAA39395)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 700 AA; 76977 MW; CFB2F4A35A306D95 CRC64;
MESAGKQDNN ATQQLPQRQQ RGNQQANKNL GKHNAQKQND SADGGPAEKK QRFGGPNAQN
QNQNQNQNGG VTGGGGAVGG PNQNKNFGNN KGGFVGNRNR NNNRAGNQNR TFPGNNNPNQ
KPNNETSKAD GPNALAKNNE PATAAAGQNQ ANQNANKGQN QRQGQNQNQN QVHGQGNQGG
PGNQGGAGNQ GGQGNQGGAG NQGNGQGFRG RNAGNNQGGG FSGGPQNQQR DNRNRSGPRP
GGGAGGAMNS TNMGGGGGGG GGGGPRGGED FFITQRLRSI SGPTFELEPV EVPTETKFSG
RNRLYVGNLT NDITDDELRE MFKPYGEISE IFSNLDKNFT FLKVDYHPNA EKAKRALDGS
MRKGRQLRVR FAPNATILRV SNLTPFVSNE LLYKSFEIFG PIERASITVD DRGKHMGEGI
VEFAKKSSAS ACLRMCNEKC FFLTASLRPC LVDPMEVNDD TDGLPEKAFN KKMPDFNQER
SIGPRFADPN SFEHEYGSRW KQLHNLFKTK QDALKRELKM EEDKLEAQME YARYEQETEL
LRQELRKREV DNERKKLEWE MREKQAEEMR KREEETMRRH QTEMQSHMNR QEEDMLRRQQ
ETLFMKAQQL NSLLDQQEGF GGGGGGNNST FDNFAGNSNS PFEVFRGNNN NNSTMIGNNA
APNTQDSFAF EFGVNNMNQG GNQRGNNGGG NNVPWGRRRF
