NOP1_CAEEL
ID NOP1_CAEEL Reviewed; 759 AA.
AC Q09314; J7H8Z6; W6RY41;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Pseudocleavage protein nop-1;
GN Name=nop-1; ORFNames=F25B5.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22887819; DOI=10.1534/genetics.112.143727;
RA Morton D.G., Hoose W.A., Kemphues K.J.;
RT "A genome-wide RNAi screen for enhancers of par mutants reveals new
RT contributors to early embryonic polarity in Caenorhabditis elegans.";
RL Genetics 192:929-942(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=7729583; DOI=10.1006/dbio.1995.1096;
RA Rose L.S., Lamb M.L., Hird S.N., Kemphues K.J.;
RT "Pseudocleavage is dispensable for polarity and development in C. elegans
RT embryos.";
RL Dev. Biol. 168:479-489(1995).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=22918944; DOI=10.1091/mbc.e12-04-0268;
RA Tse Y.C., Werner M., Longhini K.M., Labbe J.C., Goldstein B., Glotzer M.;
RT "RhoA activation during polarization and cytokinesis of the early
RT Caenorhabditis elegans embryo is differentially dependent on NOP-1 and CYK-
RT 4.";
RL Mol. Biol. Cell 23:4020-4031(2012).
CC -!- FUNCTION: Required for formation of the pseudocleavage furrow during
CC the first cleavage of the embryo and also mediates aster-induced
CC furrowing during cytokinesis. Promotes cortical recruitment of ani-1
CC and nmy-2 during pseudocleavage and cytokinesis and promotes the
CC accumulation of actin at furrowing regions. Regulates establishment of
CC embryonic cell polarity. {ECO:0000269|PubMed:22887819,
CC ECO:0000269|PubMed:22918944, ECO:0000269|PubMed:7729583}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22918944}. Cytoplasm
CC {ECO:0000269|PubMed:22918944}. Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:22918944}. Cleavage furrow
CC {ECO:0000269|PubMed:22918944}. Note=Highly concentrated in interphase
CC nuclei. As well as a significant cytoplasmic pool, a distinct cortical
CC localization is also detected, particularly at ingressing
CC pseudocleavage and cleavage furrows.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b;
CC IsoId=Q09314-1; Sequence=Displayed;
CC Name=a;
CC IsoId=Q09314-2; Sequence=VSP_053849;
CC -!- DISRUPTION PHENOTYPE: Mutant embryos do not display the anterior
CC cortical contractions associated with pseudocleavage and do not form a
CC pseudocleavage furrow. Cortical actin foci remain uniformly distributed
CC rather than the asymmetric distribution seen in wild-type embryos.
CC Approximately 20% of mutant embryos fail to hatch. Those that do hatch
CC are viable and fertile and display normal P granule localization.
CC Increased embryonic lethality in par-1, par-2 and par-3 mutants.
CC {ECO:0000269|PubMed:22887819, ECO:0000269|PubMed:22918944,
CC ECO:0000269|PubMed:7729583}.
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DR EMBL; JX293831; AFP94023.1; -; mRNA.
DR EMBL; FO081045; CCD68773.1; -; Genomic_DNA.
DR EMBL; FO081045; CDM63515.1; -; Genomic_DNA.
DR PIR; T16149; T16149.
DR RefSeq; NP_001293631.1; NM_001306702.1. [Q09314-1]
DR RefSeq; NP_498298.1; NM_065897.5. [Q09314-2]
DR AlphaFoldDB; Q09314; -.
DR BioGRID; 41066; 2.
DR IntAct; Q09314; 1.
DR STRING; 6239.F25B5.2.1; -.
DR EPD; Q09314; -.
DR PaxDb; Q09314; -.
DR PeptideAtlas; Q09314; -.
DR EnsemblMetazoa; F25B5.2a.1; F25B5.2a.1; WBGene00017774. [Q09314-2]
DR EnsemblMetazoa; F25B5.2a.2; F25B5.2a.2; WBGene00017774. [Q09314-2]
DR EnsemblMetazoa; F25B5.2b.1; F25B5.2b.1; WBGene00017774. [Q09314-1]
DR EnsemblMetazoa; F25B5.2b.2; F25B5.2b.2; WBGene00017774. [Q09314-1]
DR GeneID; 175845; -.
DR KEGG; cel:CELE_F25B5.2; -.
DR UCSC; F25B5.2.1; c. elegans. [Q09314-1]
DR CTD; 175845; -.
DR WormBase; F25B5.2a; CE01919; WBGene00017774; nop-1. [Q09314-2]
DR WormBase; F25B5.2b; CE49498; WBGene00017774; nop-1. [Q09314-1]
DR eggNOG; ENOG502TGSX; Eukaryota.
DR InParanoid; Q09314; -.
DR OMA; STMLIYP; -.
DR OrthoDB; 848512at2759; -.
DR PRO; PR:Q09314; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00017774; Expressed in adult organism and 4 other tissues.
DR GO; GO:0005938; C:cell cortex; IDA:WormBase.
DR GO; GO:0032154; C:cleavage furrow; IDA:WormBase.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0008595; P:anterior/posterior axis specification, embryo; IMP:WormBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IGI:WormBase.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell cycle; Cell division; Cytoplasm;
KW Developmental protein; Nucleus; Reference proteome.
FT CHAIN 1..759
FT /note="Pseudocleavage protein nop-1"
FT /id="PRO_0000065314"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 334..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 379..413
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 440..495
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 732..759
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..40
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 337..361
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 440..481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 324..325
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_053849"
SQ SEQUENCE 759 AA; 83663 MW; C3EF20A1450A6F76 CRC64;
MSAPKKRKSD IHSDDRDHAD HQTKKEKHWF EEKSEQNGEN RRSSIFKGFL KKVKSSTTLK
STNKSASSSK MDTIANETHL SITSESSITS VESCHSAQFP NVAKNAMLRT LVNRDSFTPV
PPPDGESVTF ILPEIGSSKL EFTSKMDDAI SIESRMSIGS CHTIDEDSAS IGSTSVFCTP
SRNPLMRNGL RMSTRSVPEN QDDMPPALFR SLCNSAVRRV PTSKSEGGGT PMRRSMRMSI
QKRNLSSKNM EDVPEEEALT VSNSSIRVDA PIAEEDEEVF GAAKEYFDNN VTSHNDSADE
GTCSMSRLST TSERASGVGG SSIFQGRRSS IFRKIFPGKS ESKDRRMSDN NQASEQYMDK
KSRRASVVSF NEFTSVSGLS VNDSASKNPK DLTESPQQPK LRKKTASSSN LSQRISSVFR
MGSNSTVKLN EYVPSPIVSQ SSRRNTLTGN SSISSGVGSI ASGTSDQGYG TIGSRSGQSI
SRCGSRRDDE GKKERTRRLL DRIIISDVPA GELLKLKIEQ VKKQKQLEMD ELFETQLTES
SSSDTSQSEN LQFDVVAVDI KADGSPFSTT AATFMDSELS KRVKEEIVSN YRPDRCGSIT
EYEPELVFVM PEQPQASLKS LNDSVSSNAD INLDHLRNVR SGLCKSIEEW KRITKMRESS
TMLIYPMFCQ SEEEWDSKGT IDAIFLMLDG ILMNTKRWMP NGKCILAGVT PNNVILLRNK
FDELKLSVAI SESDGPASSN DDFDTQSTAS TSTVFGAKI
