NOP8_YEAST
ID NOP8_YEAST Reviewed; 484 AA.
AC Q08287; D6W1S5;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=60S ribosome subunit biogenesis protein NOP8;
DE AltName: Full=Nucleolar protein 8;
GN Name=NOP8; OrderedLocusNames=YOL144W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, INTERACTION WITH NIP7 AND RRP43, AND SUBCELLULAR LOCATION.
RX PubMed=9891085; DOI=10.1128/mcb.19.2.1518;
RA Zanchin N.I.T., Goldfarb D.S.;
RT "Nip7p interacts with Nop8p, an essential nucleolar protein required for
RT 60S ribosome biogenesis, and the exosome subunit Rrp43p.";
RL Mol. Cell. Biol. 19:1518-1525(1999).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268 AND SER-370, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [6]
RP IDENTIFICATION IN A COMPLEX WITH DBP6; RSA3; URB1 AND URB2.
RX PubMed=17145778; DOI=10.1128/mcb.01523-06;
RA Rosado I.V., Dez C., Lebaron S., Caizergues-Ferrer M., Henry Y.,
RA de la Cruz J.;
RT "Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a Low-
RT molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p
RT involved in early steps of 60S ribosomal subunit biogenesis.";
RL Mol. Cell. Biol. 27:1207-1221(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-370, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268 AND SER-370, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234; SER-239; SER-268 AND
RP SER-370, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Required for 60S ribosomal subunit synthesis. May be involved
CC in assembly reactions occurring within late pre-ribosomal particles.
CC {ECO:0000269|PubMed:9891085}.
CC -!- SUBUNIT: Interacts with NIP7 and RRP43. Together with DBP6, URB1, URB2
CC and RSA3, forms an RNA-independent complex, which is required during
CC early maturation of nascent 60S ribosomal subunits.
CC {ECO:0000269|PubMed:17145778, ECO:0000269|PubMed:9891085}.
CC -!- INTERACTION:
CC Q08287; Q08962: NIP7; NbExp=4; IntAct=EBI-12135, EBI-12067;
CC Q08287; P34241: URB1; NbExp=3; IntAct=EBI-12135, EBI-26595;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:9891085}.
CC -!- MISCELLANEOUS: Present with 2170 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z74886; CAA99165.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10641.1; -; Genomic_DNA.
DR PIR; S61870; S61870.
DR RefSeq; NP_014497.1; NM_001183398.1.
DR AlphaFoldDB; Q08287; -.
DR SMR; Q08287; -.
DR BioGRID; 34273; 170.
DR ComplexPortal; CPX-1421; NOP8 60s ribosome pre-assembly complex.
DR DIP; DIP-4206N; -.
DR IntAct; Q08287; 7.
DR MINT; Q08287; -.
DR STRING; 4932.YOL144W; -.
DR iPTMnet; Q08287; -.
DR MaxQB; Q08287; -.
DR PaxDb; Q08287; -.
DR PRIDE; Q08287; -.
DR EnsemblFungi; YOL144W_mRNA; YOL144W; YOL144W.
DR GeneID; 854021; -.
DR KEGG; sce:YOL144W; -.
DR SGD; S000005504; NOP8.
DR VEuPathDB; FungiDB:YOL144W; -.
DR eggNOG; ENOG502QQ4K; Eukaryota.
DR HOGENOM; CLU_020873_1_0_1; -.
DR InParanoid; Q08287; -.
DR OMA; TKLWGYE; -.
DR BioCyc; YEAST:G3O-33534-MON; -.
DR PRO; PR:Q08287; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q08287; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IMP:SGD.
DR CDD; cd12226; RRM_NOL8; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR034138; NOP8_RRM.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis;
KW RNA-binding.
FT CHAIN 1..484
FT /note="60S ribosome subunit biogenesis protein NOP8"
FT /id="PRO_0000081678"
FT DOMAIN 7..83
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 260..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 260..275
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 276..292
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 234
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 239
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 268
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
FT MOD_RES 370
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17287358,
FT ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
SQ SEQUENCE 484 AA; 56966 MW; 30F291042433B208 CRC64;
MDSVIQKRIF VGNIFHNADD CYSELLDRFG KFGDCQDFQF EKHNHFAFID IRFNDEADFN
KLRKSFNNVK FKGNILKVDE AKPNWESTWA VQHAKDLKED IILNAKMKKK NWQHYKKMEN
VAKSWKDHKE VIAGRMREAP RKRSQLRNIT FRINVNGSLK VYKCYKTKLW GYERNKELND
LVYKFTNNFW KNGYNHIVDR LDYSRAVKTV RFKNGLKQLT VSKDENVCSG EMDSDENMSE
EEKEKNNVIL NDLLKDFDFD KPMTLNDSDE ELLTEQRKGE EEEEEEEEKE VNAPEYENVN
KTKDQSTLPQ EKPEERKEQD EGDGQEDNEF IPTFTKEIGQ GTISNTETLR NLFNPNEAEP
VSQFKLIEDS DNDIDHAKDV DVNQLEEEVS KSSDTLGLTS APVPHVSRDK DNKNFLFFPH
LQSPFLVGQT QLSKVRAPGR ETMLSNWDEE FWANRGNWTR DMRRKMKDAL KHRKRKQSKS
GLLL
