NOP9_HUMAN
ID NOP9_HUMAN Reviewed; 636 AA.
AC Q86U38; A8MY76; Q8IVF0; Q8TBS6;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Nucleolar protein 9;
GN Name=NOP9; Synonyms=C14orf21, KIAA2021;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Nagase T., Kikuno R., Ohara O.;
RT "The nucleotide sequence of a long cDNA clone isolated from human.";
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RA Li W.B., Gruber C., Jessee J., Polayes D.;
RT "Full-length cDNA libraries and normalization.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12508121; DOI=10.1038/nature01348;
RA Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA Waterston R., Hood L., Weissenbach J.;
RT "The DNA sequence and analysis of human chromosome 14.";
RL Nature 421:601-607(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ASN-308.
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [7]
RP VARIANTS [LARGE SCALE ANALYSIS] TYR-497 AND GLN-626.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q86U38-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q86U38-2; Sequence=VSP_055693, VSP_055694;
CC -!- SIMILARITY: Belongs to the NOP9 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC23117.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB095941; BAC23117.1; ALT_INIT; mRNA.
DR EMBL; BX248018; CAD62343.1; -; mRNA.
DR EMBL; AL096870; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC025332; AAH25332.1; -; mRNA.
DR CCDS; CCDS66616.1; -. [Q86U38-2]
DR CCDS; CCDS9624.1; -. [Q86U38-1]
DR RefSeq; NP_001273296.1; NM_001286367.1. [Q86U38-2]
DR RefSeq; NP_777573.1; NM_174913.2. [Q86U38-1]
DR AlphaFoldDB; Q86U38; -.
DR SMR; Q86U38; -.
DR BioGRID; 127790; 167.
DR IntAct; Q86U38; 49.
DR MINT; Q86U38; -.
DR STRING; 9606.ENSP00000267425; -.
DR iPTMnet; Q86U38; -.
DR PhosphoSitePlus; Q86U38; -.
DR SwissPalm; Q86U38; -.
DR BioMuta; NOP9; -.
DR DMDM; 34921618; -.
DR EPD; Q86U38; -.
DR jPOST; Q86U38; -.
DR MassIVE; Q86U38; -.
DR MaxQB; Q86U38; -.
DR PaxDb; Q86U38; -.
DR PeptideAtlas; Q86U38; -.
DR PRIDE; Q86U38; -.
DR ProteomicsDB; 2380; -.
DR ProteomicsDB; 69765; -. [Q86U38-1]
DR Antibodypedia; 74; 34 antibodies from 12 providers.
DR DNASU; 161424; -.
DR Ensembl; ENST00000267425.8; ENSP00000267425.3; ENSG00000196943.14. [Q86U38-1]
DR Ensembl; ENST00000396802.7; ENSP00000380020.3; ENSG00000196943.14. [Q86U38-2]
DR Ensembl; ENST00000642262.1; ENSP00000493692.1; ENSG00000285326.3. [Q86U38-2]
DR Ensembl; ENST00000644840.2; ENSP00000495668.1; ENSG00000285326.3. [Q86U38-1]
DR GeneID; 161424; -.
DR KEGG; hsa:161424; -.
DR MANE-Select; ENST00000267425.8; ENSP00000267425.3; NM_174913.3; NP_777573.1.
DR UCSC; uc001wol.3; human. [Q86U38-1]
DR CTD; 161424; -.
DR DisGeNET; 161424; -.
DR GeneCards; NOP9; -.
DR HGNC; HGNC:19826; NOP9.
DR HPA; ENSG00000196943; Low tissue specificity.
DR MIM; 618308; gene.
DR neXtProt; NX_Q86U38; -.
DR OpenTargets; ENSG00000196943; -.
DR PharmGKB; PA134867241; -.
DR VEuPathDB; HostDB:ENSG00000196943; -.
DR eggNOG; KOG2188; Eukaryota.
DR GeneTree; ENSGT00390000004964; -.
DR HOGENOM; CLU_029199_1_0_1; -.
DR InParanoid; Q86U38; -.
DR OMA; TPNGSHI; -.
DR OrthoDB; 238378at2759; -.
DR PhylomeDB; Q86U38; -.
DR TreeFam; TF327254; -.
DR PathwayCommons; Q86U38; -.
DR SignaLink; Q86U38; -.
DR BioGRID-ORCS; 161424; 565 hits in 1081 CRISPR screens.
DR ChiTaRS; NOP9; human.
DR GenomeRNAi; 161424; -.
DR Pharos; Q86U38; Tdark.
DR PRO; PR:Q86U38; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q86U38; protein.
DR Bgee; ENSG00000196943; Expressed in skeletal muscle tissue and 104 other tissues.
DR ExpressionAtlas; Q86U38; baseline and differential.
DR Genevisible; Q86U38; HS.
DR GO; GO:0030686; C:90S preribosome; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR040000; NOP9.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR PANTHER; PTHR13102; PTHR13102; 1.
DR SMART; SM00025; Pumilio; 6.
DR SUPFAM; SSF48371; SSF48371; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..636
FT /note="Nucleolar protein 9"
FT /id="PRO_0000075927"
FT REPEAT 92..123
FT /note="Pumilio 1"
FT REPEAT 189..223
FT /note="Pumilio 2"
FT REPEAT 313..348
FT /note="Pumilio 3"
FT REPEAT 351..386
FT /note="Pumilio 4"
FT REPEAT 509..544
FT /note="Pumilio 5"
FT REPEAT 547..581
FT /note="Pumilio 6"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 222..241
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 224..238
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 7
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT VAR_SEQ 520..535
FT /note="SPAGSHVLDAILTSPS -> RPGRKLLLSLGSRTRS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_055693"
FT VAR_SEQ 536..636
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_055694"
FT VARIANT 51
FT /note="P -> S (in dbSNP:rs11848295)"
FT /id="VAR_051612"
FT VARIANT 308
FT /note="S -> N (in dbSNP:rs4280164)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_024600"
FT VARIANT 497
FT /note="S -> Y (in a breast cancer sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036456"
FT VARIANT 626
FT /note="R -> Q (in a breast cancer sample; somatic mutation;
FT dbSNP:rs374412139)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036457"
FT CONFLICT 161
FT /note="A -> AE (in Ref. 4; AAH25332)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 636 AA; 69438 MW; 7978D14132A621B8 CRC64;
MGQGPRSPHK VGRRFPAGGK RGRGAKGSGR PLPGRKRQPW PPPDGRSEPA PDSHPHLSPE
ALGYFRRALS ALKEAPETGE ERDLMVHNIM KEVETQALAL STNRTGSEML QELLGFSPLK
PLCRVWAALR SNLRTVACHR CGVHVLQSAL LQLPRLLGSA AEEEEEEEED GKDGPTETLE
ELVLGLAAEV CDDFLVYCGD THGSFVVRTL LQVLGGTILE SERARPRGSQ SSEAQKTPAQ
ECKPADFEVP ETFLNRLQDL SSSFLKDIAV FITDKISSFC LQVALQVLHR KLPQFCAHLC
NAVIGYLSTR GSSVDGSPLL LFLRDQTSSR LLEQVLLVLE PPRLQSLFEE HLQGQLQTLA
AHPIANFPLQ RLLDAVTTPE LLSPVFEELS PVLEAVLAQG HPGVVIALVG ACRRVGAYQA
KVLQLLLEAF HCAEPSSRQV ACVPLFATLM AYEVYYGLTE EEGAVPAEHQ VAMAAARALG
DVTVLGSLLL QHLLHFSTPG LVLRSLGALT GPQLLSLAQS PAGSHVLDAI LTSPSVTRKL
RRRVLQNLKG QYVALACSRH GSRVLDAIWS GAALRARKEI AAELGEQNQE LIRDPFGHHV
ARNVALTTFL KRREAWEQQQ GAVAKRRRAL NSILED
