NOP9_YEAST
ID NOP9_YEAST Reviewed; 666 AA.
AC P47077; D6VWG7;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Nucleolar protein 9;
DE AltName: Full=Pumilio domain-containing protein NOP9;
GN Name=NOP9; OrderedLocusNames=YJL010C; ORFNames=J1357;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION.
RX PubMed=16544271; DOI=10.1002/yea.1353;
RA Wade C.H., Umbarger M.A., McAlear M.A.;
RT "The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains
RT over 200 genes.";
RL Yeast 23:293-306(2006).
RN [7]
RP IDENTIFICATION IN THE 90S PRE-RIBOSOME, IDENTIFICATION IN THE PRE-40S
RP RIBOSOME, FUNCTION, RNA-BINDING, AND SUBCELLULAR LOCATION.
RX PubMed=17956976; DOI=10.1261/rna.747607;
RA Thomson E., Rappsilber J., Tollervey D.;
RT "Nop9 is an RNA binding protein present in pre-40S ribosomes and required
RT for 18S rRNA synthesis in yeast.";
RL RNA 13:2165-2174(2007).
CC -!- FUNCTION: RNA-binding nucleolar protein required for pre-rRNA
CC processing. Component of the 90S pre-ribosome involved in production of
CC 18S rRNA and assembly of small ribosomal subunit. Component of the pre-
CC 40S ribosome required for release from the nucleolus.
CC {ECO:0000269|PubMed:16544271, ECO:0000269|PubMed:17956976}.
CC -!- SUBUNIT: Component of the 90S pre-ribosome. Component of the pre-40S
CC ribosome. {ECO:0000269|PubMed:17956976}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:17956976}.
CC -!- MISCELLANEOUS: Present with 2930 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the NOP9 family. {ECO:0000305}.
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DR EMBL; Z49285; CAA89301.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08783.1; -; Genomic_DNA.
DR PIR; S56781; S56781.
DR RefSeq; NP_012524.3; NM_001181444.3.
DR PDB; 5SVD; X-ray; 2.10 A; A/B=46-645.
DR PDB; 5WTX; X-ray; 3.07 A; A=1-666.
DR PDB; 5WTY; X-ray; 2.79 A; A/B=53-634.
DR PDB; 6WPI; X-ray; 3.02 A; A=46-163, A=176-645.
DR PDBsum; 5SVD; -.
DR PDBsum; 5WTX; -.
DR PDBsum; 5WTY; -.
DR PDBsum; 6WPI; -.
DR AlphaFoldDB; P47077; -.
DR SMR; P47077; -.
DR BioGRID; 33747; 263.
DR ComplexPortal; CPX-1604; Small ribosomal subunit processome, variant 1.
DR ComplexPortal; CPX-1607; Small ribosomal subunit processome, variant 2.
DR ComplexPortal; CPX-1608; Small ribosomal subunit processome, variant 3.
DR DIP; DIP-5668N; -.
DR IntAct; P47077; 12.
DR MINT; P47077; -.
DR STRING; 4932.YJL010C; -.
DR iPTMnet; P47077; -.
DR MaxQB; P47077; -.
DR PaxDb; P47077; -.
DR PRIDE; P47077; -.
DR EnsemblFungi; YJL010C_mRNA; YJL010C; YJL010C.
DR GeneID; 853445; -.
DR KEGG; sce:YJL010C; -.
DR SGD; S000003547; NOP9.
DR VEuPathDB; FungiDB:YJL010C; -.
DR eggNOG; KOG2188; Eukaryota.
DR GeneTree; ENSGT00390000004964; -.
DR HOGENOM; CLU_008720_1_1_1; -.
DR InParanoid; P47077; -.
DR OMA; TPNGSHI; -.
DR BioCyc; YEAST:G3O-31486-MON; -.
DR PRO; PR:P47077; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47077; protein.
DR GO; GO:0030686; C:90S preribosome; IDA:SGD.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IDA:SGD.
DR GO; GO:0032040; C:small-subunit processome; IPI:ComplexPortal.
DR GO; GO:0003729; F:mRNA binding; HDA:SGD.
DR GO; GO:0003723; F:RNA binding; IDA:SGD.
DR GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IC:ComplexPortal.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IMP:SGD.
DR Gene3D; 1.25.10.10; -; 3.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR040000; NOP9.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR PANTHER; PTHR13102; PTHR13102; 1.
DR SMART; SM00025; Pumilio; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Repeat; Ribosome biogenesis;
KW rRNA processing.
FT CHAIN 1..666
FT /note="Nucleolar protein 9"
FT /id="PRO_0000075939"
FT DOMAIN 23..435
FT /note="PUM-HD"
FT REPEAT 92..127
FT /note="Pumilio 1"
FT REPEAT 128..163
FT /note="Pumilio 2"
FT REPEAT 188..223
FT /note="Pumilio 3"
FT REPEAT 286..326
FT /note="Pumilio 4"
FT REPEAT 334..368
FT /note="Pumilio 5"
FT REPEAT 369..407
FT /note="Pumilio 6"
FT REPEAT 511..548
FT /note="Pumilio 7"
FT REPEAT 549..587
FT /note="Pumilio 8"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 635..666
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 641..660
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 58..73
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 79..93
FT /evidence="ECO:0007829|PDB:5SVD"
FT TURN 94..96
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 97..102
FT /evidence="ECO:0007829|PDB:5SVD"
FT TURN 104..106
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 107..116
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 119..127
FT /evidence="ECO:0007829|PDB:5SVD"
FT TURN 128..131
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 133..137
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 142..159
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 178..189
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 190..192
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 193..197
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 202..213
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 256..273
FT /evidence="ECO:0007829|PDB:5SVD"
FT STRAND 278..282
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 287..295
FT /evidence="ECO:0007829|PDB:5SVD"
FT TURN 298..300
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 301..310
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 311..313
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 319..324
FT /evidence="ECO:0007829|PDB:5SVD"
FT STRAND 328..330
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 333..342
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 346..356
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 361..371
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 373..375
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 376..380
FT /evidence="ECO:0007829|PDB:5SVD"
FT TURN 383..385
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 386..396
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 399..409
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 410..412
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 413..419
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 421..433
FT /evidence="ECO:0007829|PDB:5SVD"
FT TURN 434..438
FT /evidence="ECO:0007829|PDB:5WTY"
FT HELIX 439..450
FT /evidence="ECO:0007829|PDB:5SVD"
FT STRAND 455..457
FT /evidence="ECO:0007829|PDB:5WTY"
FT HELIX 460..465
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 467..469
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 482..497
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 499..511
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 514..521
FT /evidence="ECO:0007829|PDB:5SVD"
FT STRAND 523..525
FT /evidence="ECO:0007829|PDB:6WPI"
FT HELIX 526..533
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 536..538
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 541..551
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 552..554
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 555..559
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 564..574
FT /evidence="ECO:0007829|PDB:5SVD"
FT TURN 575..577
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 579..591
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 593..597
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 600..608
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 611..616
FT /evidence="ECO:0007829|PDB:5SVD"
FT HELIX 618..632
FT /evidence="ECO:0007829|PDB:5SVD"
SQ SEQUENCE 666 AA; 77722 MW; F6F8B3CD74DB2AB3 CRC64;
MGKTKTRGRR HQDKQRKDEF EPSSNSAKEH IQQEESTYND EAEIKETQPQ MFFGVLDREE
LEYFKQAEST LQLDAFEAPE EKFQFVTSII EEAKGKELKL VTSQITSKLM ERVILECDET
QLKDIFQSFN GVFFGLSCHK YASHVLETLF VRSAALVERE LLTPSFDNNE KEGPYVTMEN
MFLFMLNELK PHLKTMMNHQ YASHVLRLLI LILSSKTLPN STKANSTLRS KKSKIARKMI
DIKDNDDFNK VYQTPESFKS ELRDIITTLY KGFTNGAESR SDISQSTITK FREYSVDKVA
SPVIQLIIQV EGIFDRDRSF WRLVFNTADE KDPKEESFLE YLLSDPVGSH FLENVIGSAR
LKYVERLYRL YMKDRIVKLA KRDTTGAFVV RALLEHLKEK DVKQILDAVV PELSMLLNSN
MDFGTAIINA SNKQGGYLRD DVIAQLIQKY YPEKSDAKNI LESCLLLSAS TLGNTRDDWP
TAEERRRSVF LEQLIDYDDK FLNITIDSML ALPEERLIQM CYHGVFSHVV EHVLQTTRVD
IIKRKMLLNI LSKESVNLAC NVYGSHIMDK LWEFTAKLTL YKERIARALV LETEKVKNSI
YGRQVWKNWK LELYVRKMWD WKKLIKEQEF EIFPNSKPLQ PKPEKHSRER NNSKEGSAFK
KQKHYR
