ID   NOPL_SINFN              Reviewed;         338 AA.
AC   P55704;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Nodulation outer protein L;
GN   Name=nopL; OrderedLocusNames=NGR_a00770; ORFNames=y4xL;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-8, AND SUBCELLULAR LOCATION.
RX   PubMed=9680225; DOI=10.1046/j.1365-2958.1998.00920.x;
RA   Viprey V., Del Greco A., Golinowski W., Broughton W.J., Perret X.;
RT   "Symbiotic implications of type III protein secretion machinery in
RT   Rhizobium.";
RL   Mol. Microbiol. 28:1381-1389(1998).
RN   [4]
RP   FUNCTION.
RX   PubMed=14623078; DOI=10.1016/s0014-5793(03)01145-1;
RA   Bartsev A.V., Boukli N.M., Deakin W.J., Staehelin C., Broughton W.J.;
RT   "Purification and phosphorylation of the effector protein NopL from
RT   Rhizobium sp. NGR234.";
RL   FEBS Lett. 554:271-274(2003).
RN   [5]
RP   FUNCTION.
RX   PubMed=14966249; DOI=10.1104/pp.103.031740;
RA   Bartsev A.V., Deakin W.J., Boukli N.M., McAlvin C.B., Stacey G., Malnoe P.,
RA   Broughton W.J., Staehelin C.;
RT   "NopL, an effector protein of Rhizobium sp. NGR234, thwarts activation of
RT   plant defense reactions.";
RL   Plant Physiol. 134:871-879(2004).
CC   -!- FUNCTION: Putative symbiotic effector that modulates nodulation in
CC       legumes. When delivered into the plant cell, modulates the activity of
CC       signal transduction pathways that culminate in activation of PR
CC       proteins. {ECO:0000269|PubMed:14623078, ECO:0000269|PubMed:14966249}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9680225}.
CC       Note=Secreted by the type II secretion system and delivered into the
CC       host plant cytoplasm.
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DR   EMBL; U00090; AAB91935.1; -; Genomic_DNA.
DR   RefSeq; NP_444148.1; NC_000914.2.
DR   RefSeq; WP_010875118.1; NC_000914.2.
DR   AlphaFoldDB; P55704; -.
DR   STRING; 394.NGR_a00770; -.
DR   EnsemblBacteria; AAB91935; AAB91935; NGR_a00770.
DR   KEGG; rhi:NGR_a00770; -.
DR   eggNOG; ENOG502ZQ5I; Bacteria.
DR   HOGENOM; CLU_821038_0_0_5; -.
DR   OMA; HMASEDQ; -.
DR   OrthoDB; 1983353at2; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Nodulation; Plasmid; Reference proteome;
KW   Secreted.
FT   CHAIN           1..338
FT                   /note="Nodulation outer protein L"
FT                   /id="PRO_0000200967"
FT   REGION          1..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          85..158
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          187..209
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          230..259
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        85..116
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        127..142
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        238..253
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   338 AA;  37145 MW;  8AD9AE9CFDF5E5AE CRC64;
     MDINSTSPLN ASPQPDSPPP ANASAFAHQL SGFQYSPPHA ADSLLPQVEA DSPYLDTRHP
     YSQYLDSAYP YPSPCEWQHD LYTRTRERSP HPSEQRPHAR VLQGAPEHDQ DQHLEAAGPR
     EGSWQVGPSR SGPSQAGLSP SATPLNPSPP PHATDLETKH PYSQYLDWAN PSLLDWQQDL
     HTRATASPAP LTAERGRSPQ PSEQQPHARA LQVPEYDQDL IWQRVDAAGP QAGPWQVGPS
     HSGPSQARPS HAWPSSSAGA EPAELSDFVM DSGVRAWDHW FLAPHMASED QMSMLRATGL
     MPTAEVPTTT FLMMGMRHVA EFRGEGVIRI RPSVDFDI