NORA_ASPFN
ID NORA_ASPFN Reviewed; 388 AA.
AC Q00049; B8NHZ5;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 11-MAY-2016, sequence version 2.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Norsolorinic acid reductase;
DE EC=1.1.1.-;
GN Name=norA; Synonyms=aad, adh-2, AflE; ORFNames=AFLA_139310;
OS Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS / JCM 12722 / SRRC 167).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=332952;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC 167;
RX PubMed=8593042; DOI=10.1128/aem.62.2.360-366.1996;
RA Cary J.W., Wright M., Bhatnagar D., Lee R., Chu F.;
RT "Molecular characterization of an Aspergillus parasiticus dehydrogenase
RT gene, norA, located on the aflatoxin biosynthesis gene cluster.";
RL Appl. Environ. Microbiol. 62:360-366(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC 167;
RX PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT aflatoxin contamination of food and feed.";
RL Genome Announc. 3:E0016815-E0016815(2015).
CC -!- PATHWAY: Mycotoxin biosynthesis; aflatoxin biosynthesis.
CC -!- SIMILARITY: Belongs to the aldo/keto reductase family. Aldo/keto
CC reductase 2 subfamily. {ECO:0000305}.
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DR EMBL; U32377; AAC49167.1; -; Genomic_DNA.
DR EMBL; EQ963478; EED51166.1; -; Genomic_DNA.
DR RefSeq; XP_002379942.1; XM_002379901.1.
DR AlphaFoldDB; Q00049; -.
DR SMR; Q00049; -.
DR EnsemblFungi; EED51166; EED51166; AFLA_139310.
DR VEuPathDB; FungiDB:AFLA_139310; -.
DR eggNOG; KOG1575; Eukaryota.
DR OMA; SCAFRDF; -.
DR UniPathway; UPA00287; -.
DR PHI-base; PHI:7768; -.
DR Proteomes; UP000001875; Unassembled WGS sequence.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0045122; P:aflatoxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.100; -; 1.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR SUPFAM; SSF51430; SSF51430; 1.
PE 3: Inferred from homology;
KW NADP; Oxidoreductase.
FT CHAIN 1..388
FT /note="Norsolorinic acid reductase"
FT /id="PRO_0000070383"
FT ACT_SITE 74
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 233..243
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT CONFLICT 79
FT /note="S -> T (in Ref. 1; AAC49167)"
FT CONFLICT 123
FT /note="T -> A (in Ref. 1; AAC49167)"
FT CONFLICT 245
FT /note="D -> E (in Ref. 1; AAC49167)"
FT CONFLICT 339
FT /note="L -> S (in Ref. 1; AAC49167)"
SQ SEQUENCE 388 AA; 43779 MW; BF21C9CFBAB37A60 CRC64;
MVLPTAPEPP TLLGYHRILS SSAGVRVSPL CLGTMSFGNG WKGVMGECDQ ATSFNMLDTF
YESGGNFIDV ANFYQGGDSE RWVGEWMAQR QNRDEIVLST KYTMGYTMFG PQKIKSNYQG
NHTKSLRLSV KASLQKLQTD YIDLLYVHMW DFTTSVEEVM RSLNHLVANG KVLYLGVSDT
PAWLVVKCNA FARANGLTPF SVYQGHWSCA FRDFERDILP MCESEGMGLA PWGVLGRGQF
RSAEDFSREG RKMGPQDEKH RRLGEKLDQM AQQKNTKATS IAQAYVMHKA PYVFPVIGGR
KVEHLKENIE ALGLVLSEEE IREIDDAEPF DVGFPMNFLF ETPTQSYRTN MTSKDIWQLS
CNTRLETVPK QQPIEPFQGA KYFGSASK
