ID   NORA_STAAU              Reviewed;         388 AA.
AC   P0A0J7; P21191;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Quinolone resistance protein NorA;
GN   Name=norA;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2174864; DOI=10.1128/jb.172.12.6942-6949.1990;
RA   Yoshida H., Bogaki M., Nakamura S., Ubukata K., Konno M.;
RT   "Nucleotide sequence and characterization of the Staphylococcus aureus norA
RT   gene, which confers resistance to quinolones.";
RL   J. Bacteriol. 172:6942-6949(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 230-388.
RC   STRAIN=ATCC 21027 / 209-P;
RX   PubMed=2173911; DOI=10.1016/0006-291x(90)91549-8;
RA   Ohshita Y., Hiramatsu K., Yokota T.;
RT   "A point mutation in norA gene is responsible for quinolone resistance in
RT   Staphylococcus aureus.";
RL   Biochem. Biophys. Res. Commun. 172:1028-1034(1990).
CC   -!- FUNCTION: Involved in quinolone resistance. May constitute a membrane-
CC       associated active efflux pump of hydrophilic quinolones.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; D90119; BAA14147.1; -; Genomic_DNA.
DR   EMBL; M62960; AAB59089.1; -; Genomic_DNA.
DR   PIR; A36242; A36242.
DR   PIR; A37838; A37838.
DR   RefSeq; WP_001041274.1; NZ_WYDB01000004.1.
DR   AlphaFoldDB; P0A0J7; -.
DR   SMR; P0A0J7; -.
DR   BindingDB; P0A0J7; -.
DR   ChEMBL; CHEMBL5114; -.
DR   DrugCentral; P0A0J7; -.
DR   TCDB; 2.A.1.2.10; the major facilitator superfamily (mfs).
DR   OMA; LYADSFH; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR004734; Multidrug-R.
DR   InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR   Pfam; PF07690; MFS_1; 1.
DR   PRINTS; PR01035; TCRTETA.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00880; 2_A_01_02; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..388
FT                   /note="Quinolone resistance protein NorA"
FT                   /id="PRO_0000173374"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        157..177
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        269..289
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        293..313
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        355..375
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   VARIANT         352
FT                   /note="D -> A (no quinolone resistance)"
FT   CONFLICT        263
FT                   /note="M -> V (in Ref. 2; AAB59089)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        362
FT                   /note="A -> D (in Ref. 2; AAB59089)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   388 AA;  42265 MW;  C36177EAEAADAC57 CRC64;
     MNKQIFVLYF NIFLIFLGIG LVIPVLPVYL KDLGLTGSDL GLLVAAFALS QMIISPFGGT
     LADKLGKKLI ICIGLILFSV SEFMFAVGHN FSVLMLSRVI GGMSAGMVMP GVTGLIADIS
     PSHQKAKNFG YMSAIINSGF ILGPGIGGFM AEVSHRMPFY FAGALGILAF IMSIVLIHDP
     KKSTTSGFQK LEPQLLTKIN WKVFITPVIL TLVLSFGLSA FETLYSLYTA DKVNYSPKDI
     SIAITGGGIF GALFQIYFFD KFMKYFSELT FIAWSLLYSV VVLILLVFAN GYWSIMLISF
     VVFIGFDMIR PAITNYFSNI AGERQGFAGG LNSTFTSMGN FIGPLIAGAL FDVHIEAPIY
     MAIGVSLAGV VIVLIEKQHR AKLKEQNM