NORB_STAA8
ID NORB_STAA8 Reviewed; 463 AA.
AC Q2FYJ5;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Quinolone resistance protein NorB;
GN Name=norB; OrderedLocusNames=SAOUHSC_01448;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [2]
RP FUNCTION AS A MULTIDRUG RESISTANCE PUMP, AND INDUCTION BY MGRA.
RX PubMed=15774883; DOI=10.1128/jb.187.7.2395-2405.2005;
RA Truong-Bolduc Q.C., Dunman P.M., Strahilevitz J., Projan S.J., Hooper D.C.;
RT "MgrA is a multiple regulator of two new efflux pumps in Staphylococcus
RT aureus.";
RL J. Bacteriol. 187:2395-2405(2005).
RN [3]
RP INDUCTION BY NORG.
RX PubMed=17277059; DOI=10.1128/jb.01819-06;
RA Truong-Bolduc Q.C., Hooper D.C.;
RT "The transcriptional regulators norG and mgrA modulate resistance to both
RT quinolones and beta-lactams in Staphylococcus aureus.";
RL J. Bacteriol. 189:2996-3005(2007).
CC -!- FUNCTION: Multidrug efflux pump that acts independently of NorA and is
CC one of the factors that confers resistance against diverse quinolones
CC and chemical compounds. Extrudes norfloxacin, ciprofloxacin, cetrimide,
CC tetraphenylphosphonium ion (TPP), sparfloxacin, moxifloxacin and
CC ethidium bromide. Contributes also to the efflux of tetracycline.
CC {ECO:0000269|PubMed:15774883}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Down-regulated by MgrA. Up-regulated by NorG.
CC {ECO:0000269|PubMed:15774883, ECO:0000269|PubMed:17277059}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC family. {ECO:0000305}.
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DR EMBL; CP000253; ABD30537.1; -; Genomic_DNA.
DR RefSeq; WP_000414685.1; NZ_LS483365.1.
DR RefSeq; YP_499970.1; NC_007795.1.
DR AlphaFoldDB; Q2FYJ5; -.
DR SMR; Q2FYJ5; -.
DR STRING; 1280.SAXN108_1457; -.
DR EnsemblBacteria; ABD30537; ABD30537; SAOUHSC_01448.
DR GeneID; 3920226; -.
DR KEGG; sao:SAOUHSC_01448; -.
DR PATRIC; fig|93061.5.peg.1321; -.
DR eggNOG; COG0477; Bacteria.
DR HOGENOM; CLU_000960_28_3_9; -.
DR OMA; RYPRFVG; -.
DR PRO; PR:Q2FYJ5; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..463
FT /note="Quinolone resistance protein NorB"
FT /id="PRO_0000361964"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 463 AA; 49293 MW; D48070208701CD5C CRC64;
MEKPSREAFE GNNKLLIGIV LSVITFWLFA QSLVNVVPIL EDSFNTDIGT VNIAVSITAL
FSGMFVVGAG GLADKYGRIK LTNIGIILNI LGSLLIIISN IPLLLIIGRL IQGLSAACIM
PATLSIIKSY YIGKDRQRAL SYWSIGSWGG SGVCSFFGGA VATLLGWRWI FILSIIISLI
ALFLIKGTPE TKSKSISLNK FDIKGLVLLV IMLLSLNILI TKGSELGVTS LLFITLLAIA
IGSFSLFIVL EKRATNPLID FKLFKNKAYT GATASNFLLN GVAGTLIVAN TFVQRGLGYS
SLQAGSLSIT YLVMVLIMIR VGEKLLQTLG CKKPMLIGTG VLIVGECLIS LTFLPEIFYV
ICCIIGYLFF GLGLGIYATP STDTAIANAP LEKVGVAAGI YKMASALGGA FGVALSGAVY
AIVSNMTNIY TGAMIALWLN AGMGILSFVI ILLLVPKQND TQL
