ID   NORB_STAAC              Reviewed;         463 AA.
AC   Q5HFY7;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Quinolone resistance protein NorB;
GN   Name=norB; OrderedLocusNames=SACOL1475;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- FUNCTION: Multidrug efflux pump that acts independently of NorA and is
CC       one of the factors that confers resistance against diverse quinolones
CC       and chemical compounds. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; CP000046; AAW36671.1; -; Genomic_DNA.
DR   RefSeq; WP_000414684.1; NC_002951.2.
DR   AlphaFoldDB; Q5HFY7; -.
DR   SMR; Q5HFY7; -.
DR   EnsemblBacteria; AAW36671; AAW36671; SACOL1475.
DR   KEGG; sac:SACOL1475; -.
DR   HOGENOM; CLU_000960_28_3_9; -.
DR   OMA; RYPRFVG; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..463
FT                   /note="Quinolone resistance protein NorB"
FT                   /id="PRO_0000361955"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        230..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        273..293
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        299..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        403..423
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        435..455
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   463 AA;  49307 MW;  D48070208705167C CRC64;
     MEKPSREAFE GNNKLLIGIV LSVITFWLFA QSLVNVVPIL EDSFNTDIGT VNIAVSITAL
     FSGMFVVGAG GLADKYGRIK LTNIGIILNI LGSLLIIISN IPLLLIIGRL IQGLSAACIM
     PATLSIIKSY YIGKDRQRAL SYWSIGSWGG SGVCSFFGGA VATLLGWRWI FILSIIISLI
     ALFLIKGTPE TKSKSISLNK FDIKGLVLLV IMLLSLNILI TKGSELGVTS LLFITLLAIA
     IGSFSLFIVL EKRATNPLID FKLFKNKAYT GATASNFLLN GVAGTLIVAN TFVQRGLGYS
     SLQAGSLSIT YLVMVLIMIR VGEKLLQTLG CKKPMLIGTG VLIVGECLIS LTFLPEIFYV
     ICCIIGYLFF GLGLGIYATP STDTAIANAP LEKVGVAAGI YKMASALGGA FGVALSGAVY
     AIVSNMTNIY TGAMIALWLN AAMGILSFVI ILLLVPKQND TQL